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Human Endostatin Quantikine ELISA Kit

Catalog #: DNST0
22 Citations Datasheet / COA / SDS
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Control Products Available
QC81: Quantikine Immunoassay Control Set 860 for Human Endostatin
Human Endostatin ELISA Standard Curve
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Product Details
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Citations (22)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Endostatin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL)
Sensitivity
0.063 ng/mL
Assay Range
0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human Endostatin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Endostatin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Endostatin in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human Endostatin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Endostatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Endostatin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 0.7 2.02 4.14 0.76 2.13 4.21
Standard Deviation 0.05 0.12 0.15 0.06 0.13 0.24
CV% 6.9 6 3.6 7.9 6.1 5.7

Recovery

The recovery of Endostatin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 91-114
EDTA Plasma (n=4) 100 92-110
Heparin Plasma (n=4) 98 86-108
Serum (n=4) 100 93-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Endostatin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Endostatin ELISA Linearity

Scientific Data

N/A Human Endostatin ELISA Standard Curve View Larger

Human Endostatin ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endostatin

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

Entrez Gene IDs:
80781 (Human); 12822 (Mouse)
Alternate Names:
antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Endostatin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Circulating pro- and anti-angiogenic factors in multi-stage liver disease and hepatocellular carcinoma progression
    Authors: YY Joo, JW Jang, SW Lee, SH Yoo, JH Kwon, SW Nam, SH Bae, JY Choi, SK Yoon
    Sci Rep, 2019-06-24;9(1):9137.
    Species: Human
    Sample Types: Serum
  2. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
    Kidney Int Rep, 2019-06-21;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  3. Selectively activated PRP exerts differential effects on tendon stem/progenitor cells and tendon healing
    Authors: J Zhang, D Nie, K Williamson, JL Rocha, MV Hogan, JH Wang
    J Tissue Eng, 2019-01-16;10(0):2.04173E+15.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue
    Authors: M Holsti, A Wanhainen, C Lundin, M Björck, G Tegler, J Svensson, M Sund
    Eur J Vasc Endovasc Surg, 2018-04-12;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide.
    Authors: Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E
    PLoS ONE, 2015-06-15;10(6):e0130166.
    Species: Human
    Sample Types: Serum
  6. Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors.
    Authors: Marek-Trzonkowska N, Kwieczynska A, Reiwer-Gostomska M, Kolinski T, Molisz A, Siebert J
    PLoS ONE, 2015-05-07;10(5):e0126190.
    Species: Human
    Sample Types: Serum
  7. Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.
    Authors: Hutzen, Brian, Bid, Hemant K, Houghton, Peter J, Pierson, Christop, Powell, Kimerly, Bratasz, Anna, Raffel, Corey, Studebaker, Adam W
    BMC Cancer, 2014-03-19;14(0):206.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Exercise increases serum endostatin levels in female and male patients with diabetes and controls.
    Authors: Sponder M, Dangl D, Kampf S, Fritzer-Szekeres M, Strametz-Juranek J
    Cardiovasc Diabetol, 2014-01-06;13(0):6.
    Species: Human
    Sample Types: Serum
  9. Anticoagulation inhibits tumor cell-mediated release of platelet angiogenic proteins and diminishes platelet angiogenic response.
    Authors: Battinelli, Elisabet, Markens, Beth A, Kulenthirarajan, Rajesh A, Machlus, Kellie R, Flaumenhaft, Robert, Italiano, Joseph E
    Blood, 2013-09-24;123(1):101-12.
    Species: Human
    Sample Types: Whole Cells
  10. Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer.
    Authors: Leifler K, Svensson S, Abrahamsson A, Bendrik C, Robertson J, Gauldie J, Olsson A, Dabrosin C
    J Immunol, 2013-03-15;190(8):4420-30.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Antitumor activities of human placenta-derived mesenchymal stem cells expressing endostatin on ovarian cancer.
    Authors: Zheng, Lan, Zhang, Dongmei, Chen, Xianchen, Yang, Li, Wei, Yuquan, Zhao, Xia
    PLoS ONE, 2012-07-24;7(7):e39119.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer.
    Authors: Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S
    Anticancer Res., 2011-08-01;31(8):2651-5.
    Species: Human
    Sample Types: Serum
  13. In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
    Authors: Landt S, Heidecke H, Korlach S, Reuter C, Schwidde I, Barinoff J, Thill M, Sehouli J, Kummel S
    Anticancer Res., 2011-08-01;31(8):2609-15.
    Species: Human
    Sample Types: Serum
  14. Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds.
    Authors: Sun XD, Jeng L, Bolliet C, Olsen BR, Spector M
    Biomaterials, 2008-12-06;30(6):1222-31.
    Species: Goat
    Sample Types: Cell Culture Supernates
  15. Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
    Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
    Int. J. Cancer, 2008-06-15;122(12):2805-10.
    Species: Human
    Sample Types: Plasma
  16. Tamoxifen and aromatase inhibitors differentially affect vascular endothelial growth factor and endostatin levels in women with breast cancer.
    Authors: Holmes CE, Huang JC, Pace TR, Howard AB, Muss HB
    Clin. Cancer Res., 2008-05-15;14(10):3070-6.
    Species: Human
    Sample Types: Plasma
  17. Suppression of renal cell carcinoma growth and metastasis with sustained antiangiogenic gene therapy.
    Authors: Mellon MJ, Bae KH, Steding CE, Jimenez JA, Kao C, Gardner TA
    Hum. Gene Ther., 2008-05-01;19(5):487-95.
    Species: Human
    Sample Types: Serum
  18. Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo.
    Authors: Bendrik C, Robertson J, Gauldie J, Dabrosin C
    Cancer Res., 2008-05-01;68(9):3405-12.
    Species: Human
    Sample Types: Tissue Homogenates
  19. Pharmacokinetic and pharmacodynamic study of intratumoral injection of an adenovirus encoding endostatin in patients with advanced tumors.
    Authors: Li HL, Li S, Shao JY, Lin XB, Cao Y, Jiang WQ, Liu RY, Zhao P, Zhu XF, Zeng MS, Guan ZZ, Huang W
    Gene Ther., 2007-12-20;15(4):247-56.
    Species: Human
    Sample Types: Serum
  20. Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo.
    Authors: Perryman LA, Blair JM, Kingsley EA, Szymanska B, Ow KT, Wen VW, MacKenzie KL, Vermeulen PB, Jackson P, Russell PJ
    Biochem. Biophys. Res. Commun., 2006-05-11;345(3):1207-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
    Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
    Clin. Cancer Res., 2006-04-01;12(7):2055-60.
    Species: Human
    Sample Types: Serum
  22. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006-02-27;94(4):524-31.
    Species: Human
    Sample Types: Serum

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