Home / Endoglin/CD105 / Human Endoglin/CD105 Fluorescein-conjugated Antibody

Human Endoglin/CD105 Fluorescein-conjugated Antibody

Catalog #: FAB10971F
18 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB10971F-100
FAB10971F-025
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry.
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Citations (18)
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Human Endoglin/CD105 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endoglin/CD105.
Source
Monoclonal Mouse IgG1 Clone # 166707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
Glu26-Gly586
Accession # Q5T9B9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Endoglin/CD105 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB10971F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Human Endoglin/CD105 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Functionally enhanced placenta-derived mesenchymal stem cells inhibit adipogenesis in orbital fibroblasts with Graves' ophthalmopathy
    Authors: JY Kim, S Park, HJ Lee, H Lew, GJ Kim
    Stem Cell Res Ther, 2020-11-05;11(1):469.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury
    Authors: JM Sierra-Par, A Merino, M Eijken, H Leuvenink, R Ploeg, BK Møller, B Jespersen, CC Baan, MJ Hoogduijn
    Stem Cell Res Ther, 2020-08-12;11(1):352.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Immunophenotyping
  3. Enhanced Chondrogenic Capacity of Mesenchymal Stem Cells After TNF alpha Pre-treatment
    Authors: Chantal Voskamp, Wendy J. L. M. Koevoet, Rodrigo A. Somoza, Arnold I. Caplan, Véronique Lefebvre, Gerjo J. V. M. van Osch et al.
    Frontiers in Bioengineering and Biotechnology
  4. Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture
    Authors: MR Mahmoudian, F Forouzanfa, S Asgharzade, N Ghorbani
    J Ophthalmol, 2019-12-11;2019(0):2454362.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Autologous Matrix-Induced Chondrogenesis (AMIC) and AMIC Enhanced by Autologous Concentrated Bone Marrow Aspirate (BMAC) Allow for Stable Clinical and Functional Improvements at up to 9 Years Follow-Up: Results from a Randomized Controlled Study
    Authors: L de Girolam, H Schönhuber, M Viganò, C Bait, A Quaglia, G Thiebat, P Volpi
    J Clin Med, 2019-03-21;8(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs
    Authors: S Kandoi, PK L, B Patra, P Vidyasekar, D Sivanesan, V S, R K, RS Verma
    Sci Rep, 2018-08-20;8(1):12439.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
    Authors: SFH de Witte, AM Merino, M Franquesa, T Strini, JAA van Zoggel, SS Korevaar, F Luk, M Gargesha, L O'Flynn, D Roy, SJ Elliman, PN Newsome, CC Baan, MJ Hoogduijn
    Stem Cell Res Ther, 2017-06-08;8(1):140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Aging of bone marrow- and umbilical cord-derived mesenchymal stromal cells during expansion.
    Authors: de Witte S, Lambert E, Merino A, Strini T, Douben H, O'Flynn L, Elliman S, de Klein A, Newsome P, Baan C, Hoogduijn M
    Cytotherapy, 2017-04-24;19(7):798-807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation
    Authors: R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari
    BMC Cell Biol., 2017-04-21;18(1):21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Knockdown of PTRF ameliorates adipocyte differentiation and functionality of human mesenchymal stem cells
    Authors: Sergio Perez-Diaz, Beatriz Garcia-Rodriguez, Yolanda Gonzalez-Irazabal, Monica Valero, Javier Lagos-Lizan, Jose M. Arbones-Mainar
    American Journal of Physiology-Cell Physiology
  11. Proinflammatory Mediators Enhance the Osteogenesis of Human Mesenchymal Stem Cells after Lineage Commitment.
    Authors: Croes M, Oner F, Kruyt M, Blokhuis T, Bastian O, Dhert W, Alblas J
    PLoS ONE, 2015-07-15;10(7):e0132781.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Long-term expansion, enhanced chondrogenic potential, and suppression of endochondral ossification of adult human MSCs via WNT signaling modulation.
    Authors: Narcisi R, Cleary M, Brama P, Hoogduijn M, Tuysuz N, ten Berge D, van Osch G
    Stem Cell Reports, 2015-02-26;4(3):459-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Adenosine triphosphate prevents serum deprivation-induced apoptosis in human mesenchymal stem cells via activation of the MAPK signaling pathways.
    Authors: Berlier J, Rigutto S, Dalla Valle A, Lechanteur J, Soyfoo M, Gangji V, Rasschaert J
    Stem Cells, 2015-01-01;33(1):211-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2013-12-04;23(5):541-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012-10-09;7(10):e47187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2011-11-03;132(3):563-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Donor-derived mesenchymal stem cells suppress alloreactivity of kidney transplant patients.
    Authors: Crop MJ, Baan CC, Korevaar SS, Ijzermans JN, Alwayn IP, Weimar W, Hoogduijn MJ
    Transplantation, 2009-03-27;87(6):896-906.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro.
    Authors: Zhang Y, Fisher N, Newey SE
    Stem Cells Dev., 2009-03-01;18(2):359-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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