Human EGFR Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF231
Detection of EGFR in A431 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
FAQs
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Reviews (3)

Human EGFR Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EGFR (Catalog # 1095-ER)
Flow Cytometry
0.25 µg/106 cells
See below

Human EGFR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human EGFR Antibody (Catalog # AF231)

Standard: Recombinant Human EGFR Protein (Catalog # 1095-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EGFR antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EGFR in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF231, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha  in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1-3).

References
  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
 
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citations for Human EGFR Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. HEK293 producing the extracellular domain HER1: Full datasets of continuous fermentation process and metabolites analysis
    Authors: Calzadilla, L;Hernández, E;Dustet, J;Fernandez-de-Cossio-Diaz, J;León, K;Pietzke, M;Vazquez, A;Mulet, R;Boggiano, T;
    Data in brief
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  2. Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
    Authors: H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho
    Theranostics, 2018-01-01;8(2):505-517.
    Applications: Functional Assay
  3. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
    Authors: Sakanyan V, Hulin P, Alves de Sousa R, Silva V, Hambardzumyan A, Nedellec S, Tomasoni C, Loge C, Pineau C, Roussakis C, Fleury F, Artaud I
    Sci Rep, 2016-02-17;6(0):21088.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR.
    Authors: Thompson C, Bloom L, Ogiue-Ikeda M, Machida K
    BMC Biotechnol, 2015-06-26;15(0):60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Functional Assay
  5. Protein shedding in urothelial bladder cancer: prognostic implications of soluble urinary EGFR and EpCAM.
    Authors: Bryan R, Regan H, Pirrie S, Devall A, Cheng K, Zeegers M, James N, Knowles M, Ward D
    Br J Cancer, 2015-03-17;112(6):1052-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  7. Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition
    Authors: Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger
    Cellular and Molecular Bioengineering
  8. Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells
    Authors: Luca Gerosa, Christopher Chidley, Fabian Fröhlich, Gabriela Sanchez, Sang Kyun Lim, Jeremy Muhlich et al.
    Cell Systems

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Reviews for Human EGFR Biotinylated Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human EGFR Biotinylated Antibody
By Anonymous on 03/17/2021
Application: ELISA Sample Tested: Recombinant protein Species: Human

Human EGFR Biotinylated Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human EGF R/ErbB1 Biotinylated Antibody
By Anonymous on 02/22/2016
Application: ELISA Sample Tested: unknown Species: Human