Home / EDNRB/Endothelin R Type B / Human EDNRB/Endothelin R Type B Antibody

Human EDNRB/Endothelin R Type B Antibody

Catalog #: MAB4496
1 Citation Datasheet / COA / SDS
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MAB4496
MAB4496-SP
Detection of Human EDNRB/Endothelin R Type B by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EDNRB/Endothelin R Type B Antibody Summary

Species Reactivity
Human
Specificity
Detects human EDNRB/Endothelin R Type B in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 671917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human EDNRB/Endothelin R Type B
Accession # P24530
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
1-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EDNRB/Endothelin R Type B antibody by Western Blot. View Larger

Detection of Human EDNRB/Endothelin R Type B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human EDNRB/Endothelin R Type B. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human EDNRB/ Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EDNRB/Endothelin R Type B at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of EDNRB antibody in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP antibody by Flow Cytometry. View Larger

Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human EDNRB or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EDNRB Monoclonal Antibody (Catalog # MAB4496) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry EDNRB/Endothelin R Type B antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

EDNRB/Endothelin R Type B in Human Placenta. EDNRB/Endothelin R Type B was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human EDNRB/Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDNRB/Endothelin R Type B

EDNRB (Endothelin Receptor Type B) is a 50-55 kDa member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2 and 3, and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64‑Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa identity with mouse and canine EDNRB, respectively.     

Long Name
Endothelin Receptor Type B
Entrez Gene IDs
1910 (Human); 13618 (Mouse); 50672 (Rat)
Alternate Names
ABCDS; EDNRB; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor type B; ETB; ET-B; ETBR; ET-BR; ETRB; HSCR; HSCR2; WS4A

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Citation for Human EDNRB/Endothelin R Type B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Pulmonary Vascular Model From Endothelialized Whole Organ Scaffolds
    Authors: Yuan Y, Leiby KL, Greaney AM et al.
    Frontiers in Bioengineering and Biotechnology

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