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Human E-Selectin/P-Selectin (CD62E/P) Antibody

Catalog #: BBA1
18 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA1
E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells.
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Citations (18)
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Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Selectin/P-Selectin (CD62E/P) Antibody Summary

Species Reactivity
Human
Specificity
Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunoprecipitation
Pigott, R. et al. (1991) J. Immunol. 147:130.
 
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry E-Selectin (CD62E)/P-Selectin (CD62P) antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells. E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin and P-Selectin are related (44% amino acid sequence identity) adhesion molecules containing multiple short complement-like repeats and an N-terminal C‑type lectin domain in their extracellular regions. E-Selectin is expressed on activated vascular endothelial cells and P-Selectin occurs on activated platelets and endothelial cells.

Alternate Names
CD62E; ELAM; ELAM1; ESEL; Eselectin; E-Selectin/P-Selectin; LECAM2; PSelectin

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Citations for Human E-Selectin/P-Selectin (CD62E/P) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Directing and Potentiating Stem Cell-Mediated Angiogenesis and Tissue Repair by Cell Surface E-Selectin Coating
    Authors: Zhao-Jun Liu, Pirouz Daftarian, Letícia Kovalski, Bo Wang, Runxia Tian, Diego M. Castilla et al.
    PLOS ONE
  2. Identification of new multi-substituted 1H-pyrazolo[3,4-c]pyridin-7(6H)-ones as soluble guanylyl cyclase (sGC) stimulators with vasoprotective and anti-inflammatory activities
    Authors: Kintos, DP;Salagiannis, K;Sgouros, A;Nikolaropoulos, SS;Topouzis, S;Fousteris, MA;
    Bioorganic chemistry
    Species: Rat
    Sample Types: Whole Cells
    Applications: ELISA Capture
  3. Sulfonated Amphiphilic Poly(alpha)glutamate Amine-A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors
    Authors: A Krivitsky, S Pozzi, E Yeini, S Israeli Da, T Zur, S Golan, V Krivitsky, N Albeck, E Pisarevsky, P Ofek, A Madi, R Satchi-Fai
    Pharmaceutics, 2021-12-20;13(12):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  4. Microengineered perfusable 3D-bioprinted glioblastoma model for in vivo mimicry of tumor microenvironment
    Authors: L Neufeld, E Yeini, N Reisman, Y Shtilerman, D Ben-Shusha, S Pozzi, A Madi, G Tiram, A Eldar-Booc, S Ferber, R Grossman, Z Ram, R Satchi-Fai
    Science Advances, 2021-08-18;7(34):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  5. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
    Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
    Nature Communications, 2021-03-26;12(1):1912.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier
    Authors: M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas
    MBio, 2020-08-04;11(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Obesity-Altered Adipose Stem Cells Promote Radiation Resistance of Estrogen Receptor Positive Breast Cancer through Paracrine Signaling
    Authors: RA Sabol, VA Villela, A Denys, BT Freeman, AB Hartono, RM Wise, MAA Harrison, MB Sandler, F Hossain, L Miele, BA Bunnell
    Int J Mol Sci, 2020-04-15;21(8):.
  8. Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction
    Authors: JD Belcher, C Chen, J Nguyen, F Abdulla, P Zhang, H Nguyen, P Nguyen, T Killeen, SM Miescher, N Brinkman, KA Nath, CJ Steer, GM Vercellott
    PLoS ONE, 2018-04-25;13(4):e0196455.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Self-medication by orang-utans (Pongo pygmaeus) using bioactive properties of Dracaena cantleyi
    Authors: HC Morrogh-Be, I Foitová, Z Yeen, P Wilkin, R de Martin, L Rárová, K Doležal, W Nurcahyo, M Olšanský
    Sci Rep, 2017-11-30;7(1):16653.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Prenylated Polyphenols from Clusiaceae and Calophyllaceae with Immunomodulatory Activity on Endothelial Cells
    PLoS ONE, 2016-12-01;11(12):e0167361.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia
    Sci Rep, 2016-10-07;6(0):34416.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
    Authors: Roe, Kelsey, Orillo, Beverly, Verma, Saguna
    PLoS ONE, 2014-07-18;9(7):e102598.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2012-11-07;6(3):626-38.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  14. Activation of primary porcine endothelial cells induces release of porcine endogenous retroviruses.
    Authors: Cunningham DA, Dos Santos Cruz GJ, Fernandez-Suarez XM, Whittam AJ, Herring C, Copeman L, Richards A, Langford GA
    Transplantation, 2004-04-15;77(7):1071-9.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity
    Authors: Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al.
    Emerging Microbes & Infections
  16. Microfluidic kit-on-a-lid: a versatile platform for neutrophil chemotaxis assays
    Authors: Eric K. Sackmann, Erwin Berthier, Edmond W.K. Young, Miriam A. Shelef, Sarah A. Wernimont, Anna Huttenlocher et al.
    Blood
  17. Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin
    Authors: Joanna L. MacKay, Daniel A. Hammer
    Integrative Biology
  18. Potentials and capabilities of the Extracellular Vesicle (EV) Array.
    Authors: Jorgensen MM, Baek R, Varming K.
    J Extracell Vesicles

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Reviews for Human E-Selectin/P-Selectin (CD62E/P) Antibody

Average Rating: 3.3 (Based on 4 Reviews)

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Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Anonymous on 10/31/2021
Application: IHC Sample Tested: Сolon adenocarcinoma tissue Species: Human
Immunohistochemistry Human E-Selectin/P-Selectin (CD62E/P) Antibody BBA1
Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Anonymous on 11/10/2018
Application: WB Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Western blot using standard conditions on LPS or tumor secretion-stimulated HUVEC cells. No specific bands present.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Human E-Selectin/P-Selectin (CD62E/P) Antibody BBA1
Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Ruchi Gupta on 07/09/2018
Application: WB Sample Tested: Human cells Species: Human

***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Human E-Selectin/P-Selectin (CD62E/P) Antibody BBA1
Human E-Selectin/P-Selectin (CD62E/P) Antibody
By Ruchi Gupta on 08/18/2017
Application: IHC Sample Tested: Human cells Species: Human
Immunohistochemistry Human E-Selectin/P-Selectin (CD62E/P) Antibody BBA1