Human E-Selectin/CD62E Antibody
Human E-Selectin/CD62E Antibody Summary
Applications
Human E-Selectin/CD62E Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry. Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
E-Selectin/CD62E in HUVEC Human Cells. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF-a (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin/ CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
E‑Selectin/CD62E in Human Brain. E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E
E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.
Product Datasheets
Citations for Human E-Selectin/CD62E Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Glycolipid-enriched fraction of Osmanthus fragrans inhibits LPS-induced expression of inflammatory genes, COX-2, E-selectin, and Interleukin-8
Authors: T Pirker, EM Pferschy-W, E Bampali, V Bochkov, R Bauer
Journal of ethnopharmacology, 2023-03-02;0(0):116328.
Species: Human
Sample Types: Cell culture supernate
Applications: BBA16 -
The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination
Authors: Giovanni S. Offeddu, Cynthia Hajal, Colleen R. Foley, Zhengpeng Wan, Lina Ibrahim, Mark F. Coughlin et al.
Communications Biology
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Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway
Authors: Dafeng Yang, Stefan Haemmig, Haoyang Zhou, Daniel Pérez-Cremades, Xinghui Sun, Lei Chen et al.
eLife
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Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes
Authors: S Mylvaganam, M Riedl, A Vega, RF Collins, K Jaqaman, S Grinstein, SA Freeman
Cell Rep, 2020-06-23;31(12):107798.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Maternal, Fetal, and Placental Selectins in Women With Pre-eclampsia; Association With the Renin-Angiotensin-System
Authors: Hiten D. Mistry, Melissa V. Hott Ogalde, Fiona Broughton Pipkin, Geneviève Escher, Lesia O. Kurlak
Frontiers in Medicine
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Impact of single nucleotide polymorphisms in the VEGFR2 gene on endothelial cell activation under non‑uniform shear stress
Authors: Nora M. Schacher, Dorette Raaz‑Schrauder, Francesca Pasutto, Florian M. Stumpfe, Miyuki Tauchi, Barbara Dietel et al.
International Journal of Molecular Medicine
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A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling
Authors: J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin
Front Pharmacol, 2019-04-26;10(0):289.
Species: Human
Sample Types: Whole Cells
Applications: ELISA - Cell Based -
Pinocembrin protects endothelial cells from oxidized LDL-induced injury
Authors: Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li
Cytokine, 2018-06-18;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers
Authors: S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso
Infect. Immun., 2018-02-20;0(0):.
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A modular platform for targeted RNAi therapeutics
Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer
Nat Nanotechnol, 2018-01-29;0(0):.
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Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
Sci Rep, 2018-01-11;8(1):420.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe
Oncogene, 2017-12-18;0(0):.
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Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao
Cell Death Differ., 2017-12-12;0(0):.
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Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
Mol Med Rep, 2017-10-25;0(0):.
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RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity In�Vivo
Authors: L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster
Mol. Ther., 2017-10-13;0(0):.
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Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
Authors: Tra M T Bui
Reprod. Fertil. Dev, 2017-09-01;0(0):.
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The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education
Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund
Scand. J. Immunol., 2017-09-01;0(0):.
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Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice
Authors: A Tang, C Li, Z Chen, T Li
Mol Med Rep, 2017-07-28;0(0):.
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Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
Authors: K Yan, Y Shen
Mol Med Rep, 2017-07-28;0(0):.
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Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation
Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia
Allergy, 2017-07-03;0(0):.
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Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis
Authors: JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto
Transl Psychiatry, 2017-05-30;7(5):e1138.
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Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway
Authors: Gong-Jhe Wu
J. Cell. Biochem, 2017-05-16;0(0):.
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Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture
Authors: Xuejun Cheng
Dev Neurobiol, 2017-03-23;0(0):.
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Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells
Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier
Mol. Pharmacol, 2017-03-09;0(0):.
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Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model
Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak
Inflammopharmacology, 2017-03-01;0(0):.
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Amine oxidase activity regulates the development of pulmonary fibrosis
Authors: F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi
FASEB J, 2017-03-01;0(0):.
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Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine
Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng
J. Cancer Res. Clin. Oncol, 2017-02-25;0(0):.
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AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis
Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori
Respirology, 2017-02-14;0(0):.
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Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas
Int J Mol Sci, 2017-02-14;18(2):.
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Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI)
Authors: Bernhard Wernly
Eur. J. Clin. Invest, 2017-02-01;0(0):.
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3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
Authors: Minu Karthika Ganesan
Tissue Eng Part C Methods, 2017-01-01;0(0):.
Species: Human
Sample Types: Complex Sample Type
Applications: ICC -
IL-17RB(+) granulocytes are associated with airflow obstruction in asthma
Authors: Lin Li
Ann. Allergy Asthma Immunol, 2016-12-01;117(6):674-679.
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INF-? encoding plasmid administration triggers bone loss and disrupts bone marrow microenvironment
Authors: Dimitrios Agas
J. Endocrinol, 2016-12-01;0(0):.
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Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin
Authors: Trevor R F Smith
Vaccine, 2016-11-25;35(1):61-70.
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Inhibition of Adenylyl Cyclase in the Spinal Cord Alleviates Painful Diabetic Neuropathy in Zucker Diabetic Fatty Rats
Authors: Zhonghao Liu
Can J Diabetes, 2016-11-23;0(0):.
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BMP4/Id2 signaling pathway is a novel therapeutic target for late outgrowth endothelial progenitor cell-mediated endothelial injury repair
Authors: Jun Tao
Int. J. Cardiol., 2016-11-09;228(0):796-804.
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Proliferation, differentiation and migration of SCA1(-)/CD31(-) cardiac side population cells in vitro and in vivo
Authors: Simon X Liang
Int. J. Cardiol., 2016-11-09;0(0):.
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Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model
Authors: Georgi Guruli
Prostate, 2016-11-08;0(0):.
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Neutrophil gelatinase-associated lipocalin predicts myocardial dysfunction and mortality in severe sepsis and septic shock
Authors: Xiaoye Yan
Int. J. Cardiol., 2016-11-03;0(0):.
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Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress
Am J Hypertens, 2016-11-01;0(0):.
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Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats
Authors: Bruno Rodrigues
Int. J. Cardiol., 2016-10-26;0(0):.
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Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways
Authors: Paul M. Holloway, Scarlett Gillespie, Felix Becker, Shantel A. Vital, Victoria Nguyen, J. Steven Alexander et al.
Vascular Pharmacology
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Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
J Cell Mol Med, 2016-08-25;0(0):.
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Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B
Immunology, 2016-08-24;0(0):.
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C13orf31 (FAMIN) is a central regulator of immunometabolic function
Nat Immunol, 2016-08-01;0(0):.
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Critical role of fatty acid metabolism in ILC2-mediated barrier protection during malnutrition and helminth infection.
Authors: Christoph Wilhelm, Oliver J Harrison, Vanessa Schmitt, Martin Pelletier, Sean P Spencer, Joseph F Urban, Michelle Ploch, Thirumalai R Ramalinga, Richard M Siegel, Yasmine Belkaid
The Journal of Experimental Medicine, 2016-07-18;0(0):1540-9538.
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Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer
Authors: ME Legrier, I BiŠche, J Gaston, A Beurdeley, V Yvonnet, O D‚as, A Thuleau, S Chƒteau-Jo, JL Servely, S Vacher, M Lassalle, S Depil, GC Tucker, JJ Fontaine, MF Poupon, S Roman-Roma, JG Judde, D Decaudin, S Cairo, E Marangoni
Br. J. Cancer, 2015-12-22;114(2):177-87.
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TIGIT expression levels on human NK cells correlate with functional heterogeneity among healthy individuals.
Authors: Wang F, Hou H, Wu S, Tang Q, Liu W, Huang M, Yin B, Huang J, Mao L, Lu Y, Sun Z
Eur J Immunol, 2015-09-07;45(10):2886-97.
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Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis.
Authors: Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T
Mol Ther, 2015-03-27;23(6):1044-54.
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DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells
Authors: Marion Avril, Andrew J. Brazier, Martin Melcher, Sowmya Sampath, Joseph D. Smith
PLoS Pathogens
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Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
PLoS ONE, 2013-04-01;8(4):e60823.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E
Lab. Invest., 2007-09-17;87(11):1159-70.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006-11-20;203(12):2763-77.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
Blood, 2006-08-01;108(3):928-35.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development -
A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
Dev. Biol., 2006-06-15;298(1):107-17.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
Blood, 2005-11-29;107(6):2252-61.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The role of endothelial cell reactive antibodies in peripheral arterial disease.
Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
Eur J Vasc Endovasc Surg, 2005-10-14;31(2):170-5.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Differential effects of orbital and laminar shear stress on endothelial cells.
Authors: Frattini J, Kudo FA
J. Vasc. Surg., 2005-05-01;41(5):869-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
J. Vasc. Surg., 2004-03-01;39(3):613-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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HUVECs were stimulated with LPS for activation