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Human E-Cadherin ELISA Kit - Quantikine

Catalog #: DCADE0B
9 Citations Datasheet / COA / SDS
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DCADE0B
Control Products Available
QC295: Quantikine Immunoassay Control 1102 for Human E-Cadherin
Human E-Cadherin ELISA Standard Curve
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Citations (9)
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Summary Sample Values Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human E-Cadherin Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Sensitivity
0.126 ng/mL
Assay Range
0.6 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human E-Cadherin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Sample TypeMean (ng/mL)Range (ng/mL)
Serum (n=30)250127-492
EDTA Plasma (n=30)242131-479
Heparin Plasma (n=30)236116-479
Urine (n=10)60.63.03-121
Saliva (n=10)14521.4-278

Product Summary

The Quantikine™ Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, saliva, and urine. It contains HEK293-expressed recombinant human E-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human E-Cadherin.

Recovery

The recovery of human E-Cadherin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=30) 242 131-479
Heparin Plasma (n=30) 236 116-479
Saliva (n=10) 145 21.4-278
Serum (n=30) 250 127-492
Urine (n=10) 60 3.03-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human E-Cadherin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human E-Cadherin ELISA Linearity

Scientific Data

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Human E-Cadherin ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2+-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Entrez Gene IDs:
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names:
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Citations for Human E-Cadherin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical &beta-catenin-dependent mechanism
    Authors: N Ali, CB Nguyen, P Chandrakes, RF Wolf, D Qu, R May, T Goretsky, J Fazili, TA Barrett, M Li, MM Huycke, MS Bronze, CW Houchen
    Sci Rep, 2020-06-29;10(1):10578.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer
    Authors: MJ Kim, BH Chi, JJ Yoo, YM Ju, YM Whang, IH Chang
    PLoS ONE, 2019-10-22;14(10):e0223689.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Targeting the temporal dynamics of hypoxia-induced tumor-secreted factors halts tumor migration
    Authors: M Singh, XJ Tian, VS Donnenberg, AM Watson, J Zhang, LP Stabile, SC Watkins, J Xing, S Sant
    Cancer Res., 2019-04-05;0(0):.
    Species: Human, Xenograft
    Sample Types: Cell Culture Supernates
  4. Meloxicam executes its antitumor effects against hepatocellular carcinoma in COX-2- dependent and -independent pathways.
    Authors: Dong, Xiaofeng, Li, Rui, Xiu, Peng, Dong, Xuesong, Xu, Zongzhen, Zhai, Bo, Liu, Feng, Jiang, Hongchi, Sun, Xueying, Li, Jie, Qiao, Haiquan
    PLoS ONE, 2014-03-27;9(3):e92864.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Polymorphonuclear neutrophils promote dyshesion of tumor cells and elastase-mediated degradation of E-cadherin in pancreatic tumors.
    Authors: Gaida M, Steffen T, Gunther F, Tschaharganeh D, Felix K, Bergmann F, Schirmacher P, Hansch G
    Eur J Immunol, 2012-11-02;42(12):3369-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
    Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
    Oncol Rep, 2011-06-21;26(4):825-31.
    Species: Human
    Sample Types: Serum
  7. Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions.
    Authors: Streeck H, Kwon DS, Pyo A, Flanders M, Chevalier MF, Law K, Julg B, Trocha K, Jolin JS, Anahtar MN, Lian J, Toth I, Brumme Z, Chang JJ, Caron T, Rodig SJ, Milner DA, Piechoka-Trocha A, Kaufmann DE, Walker BD, Altfeld M
    Blood, 2011-03-14;117(19):5112-22.
    Species: Human
    Sample Types: Plasma
  8. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
    Authors: Hung YJ, Lee CH, Chu NF
    Diabetes Care, 2010-05-26;33(8):1840-4.
    Species: Human
    Sample Types: Plasma
  9. Elevated serum levels of soluble E-cadherin in patients with primary Sjogren's syndrome.
    Authors: Jonsson MV, Salomonsson S, Oijordsbakken G, Skarstein K
    Scand. J. Immunol., 2005-12-01;62(6):552-9.
    Species: Human
    Sample Types: Serum

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