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Human/Mouse E-Cadherin Antibody

Catalog #: AF648
78 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF648
AF648-SP
Detection of Human and Mouse E‑Cadherin by Western Blot.
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Product Details
Citations (78)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse E-Cadherin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human E-Cadherin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
Asp155-Ile707
Accession # P12830
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
0.3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
0.3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse E-Cadherin antibody by Western Blot. View Larger

Detection of Human and Mouse E‑Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E-Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of E-Cadherin antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of E‑Cadherin in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry E‑Cadherin antibody in Human Epidermoid Carcinoma Cells by Immunocytochemistry (ICC). View Larger

E‑Cadherin in Human Epidermoid Carcinoma Cells. E-Cadherin was detected in immersion fixed human epidermoid carcinoma cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry E‑Cadherin and SOX2 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

E‑Cadherin and SOX2 in BG01V Human Stem Cells. E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry E-Cadherin antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

E‑Cadherin in Human Stomach. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human and Mouse E-Cadherin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse E‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E-Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC Compatible E-Cadherin antibody in Human Stomach Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

E-Cadherin in Human Stomach Using Dual RNAscope®ISH and IHC. E-Cadherin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human stomach probed with ACD RNAScope®Probe (Catalog # 311091) and stained using ACD RNAscope®2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Immunocytochemistry/ Immunofluorescence Detection of Human E-Cadherin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human E-Cadherin by Immunocytochemistry/Immunofluorescence Impact of H. pylori infection on ES cell-derived gastric organoids. (a) HE staining of ES-cell derived gastric organoids infected with H. pylori for 12 hours. Scale bar: 200 µm. (b) Immunostaining of gastric organoids infected with H. pylori for 12 hours. “No infection” indicates that the organoid was injected with bacteria-free Brucella Broth. HP, H. pylori; E-cad, E-cadherin. Scale bar: 10 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30374120), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human E-Cadherin by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human E-Cadherin by Immunocytochemistry/Immunofluorescence Development and Characterization of an iPSC-Derived Fallopian Tube Organoid. (a) Schematic of factors involved in the differentiation of fallopian tube organoids. (b) Bright field image and H&E staining of FTE organoid at day 14. (c–e) Immunocytochemistry for FTE markers TUBB4A, FOXJ1 and PAX8 and epithelial marker CDH1 (E-Cadherin) at organoid culture day 14. (f) Immunocytochemistry for FTE markers PAX8, TUBB4A, OVGP1 and epithelial marker CDH1 at FTE organoid culture day 45, along with human fallopian tube tissue. (g) Immunocytochemistry for FTE markers TUBB4A and PAX8 at FTE organoid culture day 45. (h) Gene expression of fallopian tube markers OVGP1 (for 2 different primers), FOXJ1, TNFaIP2 and PAX8, as well as kidney markers SALL1 and FOXD1 at organoids culture day 45, human fallopian tube and kidney. The color matrix of the heat map represents the log2(Ratio) of each individual gene relative to its expression at the iPSC stage. Relative gene expression to iPSC stage (day 0) was calculated using delta delta Ct method and normalized to endogenous GAPDH level for 87iCTR-n3 iPSC line (i) H&E staining of FTE organoid at culture day 45 and day 180, and human fallopian tube tissue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28878359), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse E-Cadherin by Immunohistochemistry View Larger

Detection of Mouse E-Cadherin by Immunohistochemistry Immunohistological visualization of engineered EcN strains in tissue sections.These sections are taken from proximal colons of mice receiving different bacteria. Sectioning protocol was designed to preserve mucus and luminal content. Sections were stained with fluorescently labeled antibodies: anti-E-cadherin (green), anti-Muc2 (red), and anti-LPS (blue). The first column shows bright-field images of the sections. The last column shows an overlay of all stains. The white dotted lines represent the boundary of the epithelium and mucus layers. The leftmost parts represent the epithelium, the center parts represent the mucus layers and the rightmost parts represent the lumen (scale bar = 100 μm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31811125), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E‑Cadherin may also play a role in tumor development, as loss of E‑Cadherin has been associated with tumor invasiveness. E‑Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium‑binding domains of approximately 110 amino acids each.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Human/Mouse E-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

78 Citations: Showing 1 - 10
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  1. An Organoid-Based Preclinical Model of Human Gastric Cancer
    Authors: NG Steele, J Chakrabart, J Wang, J Biesiada, L Holokai, J Chang, LM Nowacki, J Hawkins, M Mahe, N Sundaram, N Shroyer, M Medvedovic, M Helmrath, S Ahmad, Y Zavros
    Cell Mol Gastroenterol Hepatol, 2018-09-20;7(1):161-184.
  2. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model
    Authors: Sally Dabelsteen, Emil M.H. Pallesen, Irina N. Marinova, Mathias I. Nielsen, Maria Adamopoulou, Troels B. Rømer et al.
    Developmental Cell
  3. Directed differentiation of human pluripotent stem cells into epidermal stem and progenitor cells.
    Authors: Sonya Ruiz-Torres, Paul F. Lambert, Kathryn A. Wikenheiser-Brokamp, Susanne I. Wells
    Molecular Biology Reports
  4. Keeping Candida commensal - How lactobacilli antagonize pathogenicity of Candida albicans in an in vitro gut model
    Authors: Graf K, Last A, Gratz R et al.
    Dis Model Mech
  5. Retinoic acid receptor gamma activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium
    Authors: Yasufumi Koterazawa, Michiyo Koyanagi-Aoi, Keiichiro Uehara, Yoshihiro Kakeji, Takashi Aoi
    Journal of Gastroenterology
  6. Inhibition of TGF-beta 1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-beta 1-Induced EMT in Renal Fibrosis
    Authors: Aurore Devocelle, Lola Lecru, Hélène François, Christophe Desterke, Cindy Gallerne, Pierre Eid et al.
    International Journal of Cell Biology
  7. Transplanted organoids empower human preclinical assessment of drug candidate for the clinic
    Authors: AD Westerling, EM Fast, TW Soare, S Venkatacha, M DeRan, AB Fanelli, S Kyrychenko, H Hoang, GM Corriea, W Zhang, M Yu, M Daniels, G Malojcic, XR Pan-Zhou, MW Ledeboer, JC Harmange, M Emani, TT Tibbitts, JF Reilly, P Mundel
    Science Advances, 2022-07-06;8(27):eabj5633.
  8. Single cell analysis of endometriosis reveals a coordinated transcriptional program driving immunotolerance and angiogenesis across eutopic and ectopic tissues
    Authors: Tan Y, Flynn W, Sivajothi S Et al.
    Nat Cell Biol
  9. Human mastadenovirus-B (HAdV-B)-specific E3-CR1 beta and E3-CR1 gamma glycoproteins interact with each other and localize at the plasma membrane of non-polarized airway epithelial cells
    Authors: Poornima Kotha Lakshmi Narayan, Adriana E. Kajon
    Virology
  10. A Tgfbr1/Snai1-dependent developmental module at the core of vertebrate axial elongation
    Authors: André Dias, Anastasiia Lozovska, Filip J Wymeersch, Ana Nóvoa, Anahi Binagui-Casas, Daniel Sobral et al.
    eLife
  11. Generation of multi-cellular human liver organoids from pluripotent stem cells
    Authors: Wendy L. Thompson, Takanori Takebe
    Methods in Cell Biology
  12. Generation of human colonic organoids from human pluripotent stem cells
    Authors: Abdelkader Daoud, Jorge O. Múnera
    Methods in Cell Biology
  13. Collaborating genomic, transcriptomic and microbiomic alterations lead to canine extreme intestinal polyposis
    Authors: Jin Wang, Tianfang Wang, Micah A. Bishop, John F. Edwards, Hang Yin, Stephen Dalton et al.
    Oncotarget
  14. BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial Homeostasis
    Authors: Hussein Hamade, Jasmine T. Stamps, Dalton T. Stamps, Shyam K. More, Lisa S. Thomas, Anna Y. Blackwood et al.
    Frontiers in Immunology
  15. A combined human gastruloid model of cardiogenesis and neurogenesis
    Authors: Olmsted ZT, Paluh JL.
    iScience
  16. Development of a Personalized Intestinal Fibrosis Model Using Human Intestinal Organoids Derived From Induced Pluripotent Stem Cells
    Authors: Hannah Q Estrada, Shachi Patel, Shervin Rabizadeh, David Casero, Stephan R Targan, Robert J Barrett
    Inflammatory Bowel Diseases
  17. HPV Strain Predicts Severity of Juvenile-Onset Recurrent Respiratory Papillomatosis with Implications for Disease Screening
    Authors: Mary C. Bedard, Alessandro de Alarcon, Yann-Fuu Kou, David Lee, Alexandra Sestito, Angela L. Duggins et al.
    Cancers (Basel)
  18. CDX2-induced intestinal metaplasia in human gastric organoids derived from induced pluripotent stem cells
    Authors: Takahiro Koide, Michiyo Koyanagi-Aoi, Keiichiro Uehara, Yoshihiro Kakeji, Takashi Aoi
    iScience
  19. BMAL1 Knockdown Leans Epithelial–Mesenchymal Balance toward Epithelial Properties and Decreases the Chemoresistance of Colon Carcinoma Cells
    Authors: Yuan Zhang, Aurore Devocelle, Christophe Desterke, Lucas Eduardo Botelho de Souza, Éva Hadadi, Hervé Acloque et al.
    International Journal of Molecular Sciences
  20. Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis
    Authors: Wei Rao, Shan Wang, Marcin Duleba, Suchan Niroula, Kristina Goller, Jingzhong Xie et al.
    Cell
  21. MMP20 Cleaves E-Cadherin and Influences Ameloblast Development
    Authors: John D. Bartlett, Yasuo Yamakoshi, James P. Simmer, Antonio Nanci, Charles E. Smith
    Cells Tissues Organs
  22. DR3 regulates intestinal epithelial homeostasis and regeneration after intestinal barrier injury
    Authors: Y Shimodaira, SK More, H Hamade, AY Blackwood, JP Abraham, LS Thomas, JH Miller, DT Stamps, SL Castanon, N Jacob, CW Y Ha, S Devkota, DQ Shih, SR Targan, KS Michelsen
    Cellular and Molecular Gastroenterology and Hepatology, 2023-04-01;0(0):.
  23. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019-03-05;26(10):2566-2579.e10.
  24. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica
    Authors: Y Hong, S Shan, Y Gu, H Huang, Q Zhang, Y Han, Y Dong, Z Liu, M Huang, T Ren
    Nature Communications, 2022-03-14;13(1):1309.
  25. Cytochrome P450 expression, induction and activity in human induced pluripotent stem cell-derived intestinal organoids and comparison with primary human intestinal epithelial cells and Caco-2 cells
    Authors: Aafke W. F. Janssen, Loes P. M. Duivenvoorde, Deborah Rijkers, Rosalie Nijssen, Ad A. C. M. Peijnenburg, Meike van der Zande et al.
    Archives of Toxicology
  26. Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud
    Authors: Shi, M;Crouse, B;Sundaram, N;Shakked, NP;Ester, L;Zhang, W;Janakiram, V;Kopan, R;Helmrath, MA;Bonventre, JV;McCracken, KW;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Organoid
    Applications: Immunocytochemistry
  27. Chemically-defined and scalable culture system for intestinal stem cells derived from human intestinal organoids
    Authors: Kwon, O;Lee, H;Jung, J;Son, YS;Jeon, S;Yoo, WD;Son, N;Jung, KB;Choi, E;Lee, IC;Kwon, HJ;Kim, C;Lee, MO;Cho, HS;Kim, DS;Son, MY;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry/Immunofluorescence
  28. Gasdermin D promotes hyperinflammation and immunopathology during severe influenza A virus infection
    Authors: Rosli, S;Harpur, CM;Lam, M;West, AC;Hodges, C;Mansell, A;Lawlor, KE;Tate, MD;
    Cell death & disease
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. The minichromosome maintenance complex drives esophageal basal zone hyperplasia
    Authors: Rochman, ME;Rochman, Y;Caldwell, JM;Mack, L;Besse, JA;Manes, NP;Yoon, SH;Shoda, T;Nita-Lazar, A;Rothenberg, M;
    JCI insight
    Species: Human, Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  30. DR3 regulates intestinal epithelial homeostasis and regeneration after intestinal barrier injury
    Authors: Y Shimodaira, SK More, H Hamade, AY Blackwood, JP Abraham, LS Thomas, JH Miller, DT Stamps, SL Castanon, N Jacob, CW Y Ha, S Devkota, DQ Shih, SR Targan, KS Michelsen
    Cellular and Molecular Gastroenterology and Hepatology, 2023-04-01;0(0):.
  31. Pre-hyperglycemia immune cell trafficking underlies subclinical diabetic cataractogenesis
    Authors: E Ranaei Pir, Y Zhang, A Barakat, M Naseri, C Russmann, A Hafezi-Mog
    Journal of Biomedical Science, 2023-01-24;30(1):6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. A combined human gastruloid model of cardiogenesis and neurogenesis
    Authors: Olmsted ZT, Paluh JL.
    iScience
  33. Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids
    Authors: K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi
    Stem Cell Reports, 2022-03-03;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  34. Human iPSC-derived fallopian tube organoids with BRCA1 mutation recapitulate early-stage carcinogenesis
    Authors: N Yucer, R Ahdoot, MJ Workman, AH Laperle, MS Recouvreux, K Kurowski, DJ Naboulsi, V Liang, Y Qu, JT Plummer, SA Gayther, S Orsulic, BY Karlan, CN Svendsen
    Cell Reports, 2021-12-28;37(13):110146.
    Species: Human
    Sample Types: Organoid, Whole Cells
    Applications: ICC, IHC
  35. Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells
    Authors: AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells
    Cell Stem Cell, 2021-12-01;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. A Wnt-mediated phenotype switch along the epithelial-mesenchymal axis defines resistance and invasion downstream of ionising radiation in oral squamous cell carcinoma
    Authors: F Zolghadr, N Tse, D Loka, G Joun, S Meppat, V Wan, H Zoellner, M Xaymardan, CS Farah, JG Lyons, E Hau, E Patrick, N Seyedasli
    British Journal of Cancer, 2021-03-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Organoid, Whole Cells
    Applications: Flow Cytometry, IHC, Western Blot
  37. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
    Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
    Nature Communications, 2021-01-11;12(1):262.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  38. Patient-Derived Organotypic Epithelial Rafts Model Phenotypes in Juvenile-Onset Recurrent Respiratory Papillomatosis
    Authors: MC Bedard, MG Brusadelli, A Carlile, S Ruiz-Torre, H Lodin, D Lee, M Kofron, PF Lambert, A Lane, N Ameziane, EM Bahassi, KA Wikenheise, A de Alarcon, DF Smith, SI Wells
    Viruses, 2021-01-06;13(1):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  39. Modeling Intestinal Epithelial Response to Interferon-&gamma in Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids
    Authors: MJ Workman, E Troisi, SR Targan, CN Svendsen, RJ Barrett
    International Journal of Molecular Sciences, 2020-12-30;22(1):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  40. Claudin-18 Loss Alters Transcellular Chloride Flux but not Tight Junction Ion Selectivity in Gastric Epithelial Cells
    Authors: TJ Caron, KE Scott, N Sinha, S Muthupalan, M Baqai, LH Ang, Y Li, JR Turner, JG Fox, SJ Hagen
    Cell Mol Gastroenterol Hepatol, 2020-10-16;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. Repair of acute liver damage with immune evasive hESC derived hepato-blasts
    Authors: J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li
    Stem Cell Res, 2020-09-26;49(0):102010.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  42. Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover
    Authors: KL Alexander, CA Serrano, A Chakrabort, M Nearing, LN Council, A Riquelme, M Garrido, SL Bellis, LE Smythies, PD Smith
    J. Biol. Chem., 2020-08-06;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: ICC, IHC
  43. Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut
    Authors: P Praveschot, AM Duraj-That, I Gelfat, F Bahl, DB Chou, NS Joshi
    Nat Commun, 2019-12-06;10(1):5580.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  44. miR-221-5p regulates proliferation and migration in human prostate cancer cells and reduces tumor growth in vivo
    Authors: M Kiener, L Chen, M Krebs, J Grosjean, I Klima, C Kalogirou, H Riedmiller, B Kneitz, GN Thalmann, E Snaar-Jaga, M Spahn, M Kruithof-d, E Zoni
    BMC Cancer, 2019-06-25;19(1):627.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  45. Human Pluripotent Stem Cell-Derived Kidney Model for Nephrotoxicity Studies.
    Authors: Bajaj P, Rodrigues A, Steppan C, Engle S, Mathialagan S, Schroeter T
    Drug Metab Dispos, 2018-08-31;46(11):1703-1711.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  46. Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation
    Authors: T Topal, X Hong, X Xue, Z Fan, N Kanetkar, JT Nguyen, J Fu, CX Deng, PH Krebsbach
    Sci Rep, 2018-08-28;8(1):12977.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. IDENTIFICATION OF PIPERAZINYLBENZENESULFONAMIDES AS NEW INHIBITORS OF CLAUDIN-1 TRAFFICKING AND HEPATITIS C VIRUS ENTRY
    Authors: L Riva, OR Song, J Prentoe, F Helle, L L'homme, CH Gattolliat, A Vandeputte, L Fénéant, S Belouzard, TF Baumert, T Asselah, J Bukh, P Brodin, L Cocquerel, Y Rouillé, J Dubuisson
    J. Virol., 2018-04-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  48. A novel transforming growth factor beta-induced long noncoding RNA promotes an inflammatory microenvironment in human intrahepatic cholangiocarcinoma
    Authors: A Merdrignac, G Angenard, C Allain, K Petitjean, D Bergeat, P Bellaud, A Fautrel, B Turlin, B Clément, S Dooley, L Sulpice, K Boudjema, C Coulouarn
    Hepatol Commun, 2018-01-30;2(3):254-269.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  49. Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips.
    Authors: Michael J Workman, John P Gleeson, Elissa J Troisi, Hannah Q Estrada, S Jordan Kerns, Christopher D Hinojosa, Geraldine A Hamilton, Stephan R Targan, Clive N Svendsen, Robert J Barrett
    Cellular and Molecular Gastroenterology and Hepatology, 2017-12-29;0(0):2352-345X.
    Species: Human
    Sample Types:
    Applications: ICC
  50. Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
    Authors: L Yang, TQ Zha, X He, L Chen, Q Zhu, WB Wu, FQ Nie, Q Wang, CS Zang, ML Zhang, J He, W Li, W Jiang, KH Lu
    Oncol. Rep., 2017-11-09;39(1):53-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  51. Directed Differentiation of Human Induced Pluripotent Stem Cells into Fallopian Tube Epithelium
    Authors: N Yucer, M Holzapfel, T Jenkins Vo, L Lenaeus, L Ornelas, A Laury, D Sareen, R Barrett, BY Karlan, CN Svendsen
    Sci Rep, 2017-09-06;7(1):10741.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. miR-151a induces partial EMT by regulating E-cadherin in NSCLC cells
    Authors: I Daugaard, KJ Sanders, A Idica, K Vittayaruk, M Hamdorf, JD Krog, R Chow, D Jury, LL Hansen, H Hager, P Lamy, CL Choi, D Agalliu, DG Zisoulis, IM Pedersen
    Oncogenesis, 2017-07-31;6(7):e366.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
    Authors: G Abagnale, A Sechi, M Steger, Q Zhou, CC Kuo, G Aydin, C Schalla, G Müller-New, M Zenke, IG Costa, P van Rijn, A Gillner, W Wagner
    Stem Cell Reports, 2017-07-27;9(2):654-666.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017-03-01;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  55. A process engineering approach to increase organoid yield
    Authors: N Arora, J Imran Also, JW Guggenheim, M Mak, J Munera, JM Wells, RD Kamm, HH Asada, SY Shvartsman, LG Griffith
    Development, 2017-02-07;144(6):1128-1136.
    Species: Human
    Sample Types: Spheroid
    Applications: IHC
  56. Wnt/?-catenin promotes gastric fundus specification in mice and humans
    Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
    Nature, 2017-01-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  57. Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level.
    Authors: Liu D, Xiong H, Ellis A, Northrup N, Dobbin K, Shin D, Zhao S
    PLoS Genet, 2015-06-01;11(6):e1005277.
    Species: Canine
    Sample Types: Whole Cells
    Applications: IHC-P
  58. Molecular homology and difference between spontaneous canine mammary cancer and human breast cancer.
    Authors: Liu D, Xiong H, Ellis A, Northrup N, Rodriguez C, O'Regan R, Dalton S, Zhao S
    Cancer Res, 2014-07-31;74(18):5045-56.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-P
  59. Novel human renal proximal tubular cell line for the production of complex proteins.
    Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
    J Biotechnol, 2014-02-16;176(0):29-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  60. Penton-dodecahedral particles trigger opening of intercellular junctions and facilitate viral spread during adenovirus serotype 3 infection of epithelial cells.
    Authors: Lu Z, Wang H, Zhang Y, Cao H, Li Z, Fender P, Lieber A
    PLoS Pathog, 2013-10-31;9(10):e1003718.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  61. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
    Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
    PLoS ONE, 2012-02-21;7(2):e31624.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells.
    Authors: Li H, Wang H, Wang F, Gu Q, Xu X
    PLoS ONE, 2011-08-10;6(8):e23322.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  63. Tissue engineering of oral dysplasia.
    Authors: Gaballah K, Costea DE, Hills A, Gollin SM, Harrison P, Partridge M
    J. Pathol., 2008-07-01;215(3):280-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  64. Downregulation of Rap1GAP contributes to Ras transformation.
    Authors: Tsygankova OM, Prendergast GV, Puttaswamy K, Wang Y, Feldman MD, Wang H, Brose MS, Meinkoth JL
    Mol. Cell. Biol., 2007-07-23;27(19):6647-58.
    Species: Human, Ovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  65. Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis.
    Authors: Tamai R, Asai Y, Ogawa T
    Infect. Immun., 2005-10-01;73(10):6290-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  66. Methyl Donors Reduce Cell Proliferation by Diminishing Erk-Signaling and NFkB Levels, While Increasing E-Cadherin Expression in Panc-1 Cell Line
    Authors: Eva Kiss, Gertrud Forika, Magdolna Dank, Tibor Krenacs, Zsuzsanna Nemeth
    International Journal of Molecular Sciences
  67. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis
    Authors: Cui W, Sathyanarayan A, Lopresti M et al.
    Autophagy
  68. Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells
    Authors: Hiroyuki Koike, Kentaro Iwasawa, Rie Ouchi, Mari Maezawa, Masaki Kimura, Asuka Kodaka et al.
    Nature Protocols
  69. Neonatal Injury Increases Gut Permeability by Epigenetically Suppressing E-Cadherin in Adulthood
    Authors: KT Kline, H Lian, XS Zhong, X Luo, JH Winston, Y Cong, TC Savidge, RH Dashwood, DW Powell, Q Li
    J. Immunol., 2019-12-30;0(0):.
  70. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids
    Authors: Kyle W. McCracken, Emily M. Catá, Calyn M. Crawford, Katie L. Sinagoga, Michael Schumacher, Briana E. Rockich et al.
    Nature
  71. Generation of human elongating multi-lineage organized cardiac gastruloids
    Authors: Zachary T. Olmsted, Maria Belen Paredes-Espinosa, Janet L. Paluh
    STAR Protocols
  72. Role of BCL9L in transforming growth factor-beta (TGF-beta )-induced epithelial-to-mesenchymal-transition (EMT) and metastasis of pancreatic cancer
    Authors: Giuseppina Sannino, Nicole Armbruster, Mona Bodenhöfer, Ursula Haerle, Diana Behrens, Malte Buchholz et al.
    Oncotarget
  73. Evaluating the origin and virulence of a Helicobacter pylori cagA-positive strain isolated from a non-human primate
    Authors: Kana Hashi, Chihiro Imai, Koji Yahara, Kamrunnesa Tahmina, Takeru Hayashi, Takeshi Azuma et al.
    Scientific Reports
  74. Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells
    Authors: Luoman Chen, Stephen Dalton
    STAR Protocols
  75. Induced hepatic stem cells are suitable for human hepatocyte production
    Authors: Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al.
    iScience
  76. Terbium-based time-gated Forster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes
    Authors: Linden S, Singh MK, Wegner KD et al.
    Dalton Trans.
  77. A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins
    Authors: Katrin Luckert, Taranjit S. Gujral, Marina Chan, Mark Sevecka, Thomas O. Joos, Peter K. Sorger et al.
    Science Signaling
  78. Activatable Zymography Probes Enable In Situ Localization of Protease Dysregulation in Cancer
    Authors: Ava P. Soleimany, Jesse D. Kirkpatrick, Susan Su, Jaideep S. Dudani, Qian Zhong, Ahmet Bekdemir et al.
    Cancer Research

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Human/Mouse E-Cadherin Antibody
By Anonymous on 10/24/2023
Application: IHC Sample Tested: human colorectal cancer tissue Species: Human

IHC staining for CDH1 was performed on human colon cancer tissue

Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Human/Mouse E-Cadherin Antibody
By Anonymous on 01/22/2019
Application: IHC Sample Tested: embryonic brain/ choroid plexus Species: Mouse

The antibody did not give any specific staining on mouse paraffin sections of choroid plexus, Epitope retrieval: Sodium citrate
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Human/Mouse E-Cadherin Antibody
By Anonymous on 12/10/2017
Application: IHC Sample Tested: Kidney cancer tissue Species: Human
Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Human/Mouse E-Cadherin Antibody
By Anonymous on 08/29/2016
Application: IHC Sample Tested: skin tumor Species: Human

Antigen retrieval: 10mM Citrate pH6
Antibody dilution: 1:400
Secondary antibody: Alexa 647 donkey anti-goat secondary antibody

Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Human/Mouse E-Cadherin Antibody
By Anonymous on 08/22/2016
Application: IHC Sample Tested: Stomach tissue Species: Mouse

Antigen retrieval: 10mM Citrate buffer pH6.
Antibody dilution: 1:400
Secondary antibody: CY3 conjugated donkey anti-goat secondary antibody @ 1:300

Immunohistochemistry Human/Mouse E-Cadherin Antibody AF648
Human/Mouse E-Cadherin Antibody
By Anonymous on 07/19/2016
Application: IHC Sample Tested: Hindgut Species: Human