Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody
Human Dopamine D1R/DRD1 Alexa Fluor® 488-conjugated Antibody Summary
Met1-Thr446
Accession # P21728
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
Background: Dopamine D1R/DRD1
Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed with PBS. Cells concentration was adjusted to 10^4 cells per uL. 10^6 of cells were aliquoted into centrifuge tubes for staining.
1uL DRD1 antibody was added to cell suspension. Cells were incubated on ice for 30 mins protected from light. Following incubation, cells were washed twice with PBS and then fixed at room temperature for 30 mins protected from light. Cells were washed twice with permeabilization buffer and resuspended in 500 uL PBS for flow cytometry acquisition.
Black graph depicts unstained PBMCs. Red graph depicts DRD1 stained PBMCs.
