Human DLL4 Antibody Summary
Ser27-Pro524
Accession # Q9NR61
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DLL4 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).
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DLL4 in HUVEC Human Cells. DLL4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DLL4
Delta-like ligand 4 (DLL4) is a transmembrane protein that contains one DSL domain and eight tandem EGF-like repeats. DLL4 is expressed on arterial endothelial cells where it signals through Notch 1 and Notch 4. DLL4 expression is induced by VEGF and regulates the proliferation of endothelial tip cells during vascular sprouting. DLL4 blockade as well as overexpression can suppress tumor growth by promoting dysregulated angiogenesis. Within the extracellular domain, human DLL4 shares 85% amino acid sequence identity with mouse and rat DLL4.
Product Datasheets
Citations for Human DLL4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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NOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells
Authors: A Kumar, JH Lee, K Suknuntha, SS D'Souza, AS Thakur, II Slukvin
J. Immunol., 2018-12-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Concurrent Treatment with Anti-DLL4 Enhances Antitumor and Proapoptotic Efficacy of a ?-Secretase Inhibitor in Gastric Cancer
Authors: M Kang, Y Zhang, X Jin, G Chen, Y Huang, D Wu, G Li, J Shan, P Huang, J Chen
Transl Oncol, 2018-03-16;11(3):599-608.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Staining was done on E10.5 mouse sections (pharyngeal mesoderm).
Note the arteries being positive and adjacent veins staining negative for Dll4.
