Home / DFF40/CAD / Human DFF40/CAD Antibody

Human DFF40/CAD Antibody

Catalog #: MAB6289
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6289
MAB6289-SP
Detection of Human DFF40/CAD by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DFF40/CAD Antibody Summary

Species Reactivity
Human
Specificity
Detects human DFF40/CAD in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 609809
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DFF40/CAD
Met1-Gln338
Accession # O76075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DFF40/CAD antibody by Western Blot. View Larger

Detection of Human DFF40/CAD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DFF40/CAD Monoclonal Antibody (Catalog # MAB6289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DFF40/CAD at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry DFF40/CAD antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

DFF40/CAD in HeLa Human Cell Line. DFF40/CAD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human DFF40/CAD Monoclonal Antibody (Catalog # MAB6289) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DFF40/CAD

DNA fragmentatation factor 40 kDa subunit (DFF40), also known as CAD, is a member of the CIDE domain-containing proteins. It is an apoptotic nuclease that is maintained in an inactive state by association with DFF45. Apoptotic signaling induces DFF45 cleavage and the release of DFF40. Active DFF40 exists as an oligomer and generates double stranded breaks in dsDNA. Alternate splicing of human DFF40 generates isoforms with substitutions and truncations either directly following the CIDE domain (which is aa 4‑80) or at the C-terminus. Full length human DFF40 (aa 1‑338) shares 76% and 74% aa sequence identity with mouse and rat DFF40, respectively.

Long Name
DNA Fragmentation Factor, Subunit beta
Entrez Gene IDs
1677 (Human); 13368 (Mouse); 84359 (Rat)
Alternate Names
CAD; CADCaspase-activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; CPAN; CPANDNA fragmentation factor 40 kDa subunit; DFF2; DFF40; DFF-40DFF40DFF2DNA fragmentation factor subunit beta; DFFB; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); EC 3.-

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Citation for Human DFF40/CAD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Histone H1 quantity determines the efficiencies of apoptotic DNA fragmentation and chromatin condensation
    Authors: M Kijima, R Mizuta
    Biomed. Res., 2019-01-01;40(1):51-56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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