Human Desmin Antibody

Detection of Desmin in Human Skeletal Muscle via seqIF™ staining on COMET™​ Desmin was detected in immersion fixed paraffin-embedded sections of human Skeletal Muscle using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder.

Detection of Human, Mouse and Rat Desmin by Western Blot. Western Blot shows lysates of RD human rhabdomyosarcoma cell line, C2C12 mouse myoblast cell line and rat heart tissue. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Desmin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Desmin in human heart Desmin was detected in immersion fixed paraffin-embedded sections of heart using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Desmin in human skeletal muscle Desmin was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Desmin in RD cells and A172 cells. Desmin was detected in immersion fixed RD cells (Positive) and A172 human glioblastoma cell line (Positive) using Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # mab11607) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Desmin by Simple Western^TM. Simple Western shows lysates of RD human rhabdomyosarcoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Desmin at approximately 59 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11607). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.