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Human DC-LAMP Lumenal Domain Antibody

Catalog #: AF4087
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4087
AF4087-SP
Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DC-LAMP Lumenal Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑LAMP Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DC‑LAMP Lumenal Domain
Asp21-Thr381
Accession # EAW78337
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human DC-LAMP Lumenal Domain
Flow Cytometry
0.25 µg/106 cells
Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DC‑LAMP antibody in Human Mature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells treated with 20 ng/mL Recombinant Human IL-4 (204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-LAMP

DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50‑70 kDa member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It has a 254 aa lumenal N‑terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively.

Long Name
Dendritic Cell-lysosome-associated Membrane Glycoprotein
Entrez Gene IDs
27074 (Human); 239739 (Mouse); 303801 (Rat)
Alternate Names
CD208 antigen; CD208; DCLAMP; DC-LAMP; DCLAMPDC-lysosome-associated membrane glycoprotein; LAMP; LAMP3; LAMP-3; lysosomal-associated membrane protein 3DC LAMP; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403; TSC403CD208

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Citations for Human DC-LAMP Lumenal Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer
    Authors: Á de Mingo P, A Gardner, S Hiebler, H Soliman, HS Rugo, MF Krummel, LM Coussens, B Ruffell
    Cancer Cell, 2018-01-08;33(1):60-74.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Loss of lysosome-associated membrane protein 3 (LAMP3) enhances cellular vulnerability against proteasomal inhibition
    Authors: Dominguez-Bautista JA, Klinkenberg M, Brehm N et al.
    Eur. J. Cell Biol.

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