Human DC-LAMP Lumenal Domain Antibody

Catalog #: AF4087
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4087
AF4087-SP

Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry.

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All DC-LAMP Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DC-LAMP Lumenal Domain Antibody Summary

Species Reactivity

Human

Specificity

Detects human DC‑LAMP Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DC‑LAMP Lumenal Domain
Asp21-Thr381
Accession # EAW78337

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human DC-LAMP Lumenal Domain

Flow Cytometry

0.25 µg/10⁶ cells

Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺ cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DC‑LAMP in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺cells treated with 20 ng/mL Recombinant Human IL-4 (204-IL) and 50 ng/mL Recombinant Human GM-CSF (215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (F0108).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-LAMP

DC-LAMP (dendritic cell lysosome-associated membrane protein: also known as CD208 and LAMP3) is a 50‑70 kDa member of the LAMP family of proteins. Mature human DC-LAMP is a 389 amino acid (aa) type I transmembrane protein. It has a 254 aa lumenal N‑terminus and a short 14 aa cytoplasmic tail. The molecule is found in type II pneumocytes, interdigitating DC, and various tumors. DC-LAMP serves as a marker of human mature DC, and it may play a role in normal lysosome and endosome function. Over the region used as immunogen, human DC-LAMP shares 72% and 55% aa sequence identity with canine and mouse DC-LAMP, respectively.

Long Name

Dendritic Cell-lysosome-associated Membrane Glycoprotein

Entrez Gene IDs

27074 (Human); 239739 (Mouse); 303801 (Rat)

Alternate Names

CD208 antigen; CD208; DCLAMP; DC-LAMP; DCLAMPDC-lysosome-associated membrane glycoprotein; LAMP; LAMP3; LAMP-3; lysosomal-associated membrane protein 3DC LAMP; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403; TSC403CD208

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human DC-LAMP Lumenal Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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