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Human/Cynomolgus Monkey B7-H6 Antibody

Catalog #: MAB10162 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10162-100
MAB10162-SP
Detection of Cynomolgus Monkey B7‑H6 by Western Blot.
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Human/Cynomolgus Monkey B7-H6 Antibody Summary

Species Reactivity
Human, Cynomolgus Monkey
Specificity
Detects human and cynomolgus monkey B7‑H6 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1002910
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived Cynomolgus Monkey B7‑H6
Asp25-Ser262
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Cynomolgus Monkey B7-H6 antibody by Western Blot. View Larger

Detection of Cynomolgus Monkey B7‑H6 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or transfected with cynomologus monkey B7-H6. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7-H6 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of B7-H6 antibody in HEK293 Human Cell Line Transfected with Human B7-H6 and eGFP antibody by Flow Cytometry. View Larger

Detection of B7-H6 in HEK293 Human Cell Line Transfected with Human B7-H6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Human B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of B7-H6 antibody in HEK293 Human Cell Line Transfected with Cynomologus Monkey B7-H6 and eGFP antibody by Flow Cytometry. View Larger

Detection of B7-H6 in HEK293 Human Cell Line Transfected with Cynomologus Monkey B7-H6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Cynomolgus Monkey B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H6

B7-H6 is a 55 kDa glycosylated member of the B7 family of immune costimulatory proteins (1, 2). Mature B7-H6 consists of an extracellular domain (ECD) that with one Ig-like V domain and one Ig-like C1 domain, a transmembrane segment, and a cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, cynomolgous B7-H6 shares 81%, 81%, 77% and 80% amino acid sequence identity with chimpanzee, orangutan, gibbon, and human B7-H6, respectively. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). B7-H6 is up-regulated on inflammatory monocytes and neutrophils, and a 30 kDa soluble fragment can be released by ADAM-10 or ADAM-17 mediated shedding (8, 9). The expression of B7-H6 on tumor cells correlates with cancer progression and patient's survival in human ovarian cancer (10).

References
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
  4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
  5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. USA 108:6223.
  6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
  7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.
  8. Matta, J. et al. (2013) Blood 122:394.
  9. Schlecker, E. et al. (2014) Cancer Res. 74:3429.
  10. Zhou, Y. et al. (2015) Int. J. Clin. Exp. Pathol. 8:9428.
Long Name
B7 Homolog 6
Entrez Gene IDs
374383 (Human); 102121659 (Cynomolgus Monkey)
Alternate Names
B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1

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