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Human CXCR3 Fluorescein-conjugated Antibody

Catalog #: FAB160F
24 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB160F-025
FAB160F-100
Detection of CXCR3 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (24)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR3.
Source
Monoclonal Mouse IgG1 Clone # 49801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR3
Met1-Leu368
Accession # P49682
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR3 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CXCR3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB160F) and Mouse Anti-Human CD3e Alexa Fluor® 700-conjugated Monoclonal Antibody (FAB100N). Quadrant markers were set based on control antibody staining (IC002F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CXCR3 in RPMI8226 Human Cell Line by Flow Cytometry. RPMI8226 human myeloma cell line was stained with Mouse Anti-Human CXCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB160F, filled histogram) or Mouse IgG1 isotype control antibody (IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR3

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Entrez Gene IDs
2833 (Human); 12766 (Mouse); 84475 (Rat)
Alternate Names
CD183 antigen; CD183; chemokine (C-X-C motif) receptor 3; chemokine (C-X-C) receptor 3; CKR-L2IP-10 receptor; CMKAR3; C-X-C chemokine receptor type 3; CXCR3; CXC-R3; CXCR-3; G protein-coupled receptor 9CD182; GPR9; GPR9Mig-R; Interferon-inducible protein 10 receptor; IP10 receptor; IP10-R; Mig receptor; MigR

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Citations for Human CXCR3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
    Authors: L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez
    International Journal of Molecular Sciences, 2021-04-09;22(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Significance of IL-17A-producing CD8+CD103+ skin resident memory T cells in psoriasis lesion and their possible relationship to clinical course
    Authors: K Kurihara, T Fujiyama, P Phadungsak, T Ito, Y Tokura
    J. Dermatol. Sci., 2019-06-20;95(1):21-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016-10-19;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A distinct plasmablast and na�ve B-cell phenotype in primary immune thrombocytopenia
    Haematologica, 2016-03-11;101(6):698-706.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014-05-16;192(12):5776-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Phase I clinical trial of multiple-peptide vaccination for patients with advanced biliary tract cancer.
    Authors: Aruga, Atsushi, Takeshita, Nobuhiro, Kotera, Yoshihit, Okuyama, Ryuji, Matsushita, Norimasa, Ohta, Takehiro, Takeda, Kazuyosh, Yamamoto, Masakazu
    J Transl Med, 2014-03-07;12(0):61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Plasmablasts as migratory IgG-producing cells in the pathogenesis of neuromyelitis optica.
    Authors: Chihara, Norio, Aranami, Toshimas, Oki, Shinji, Matsuoka, Takako, Nakamura, Masakazu, Kishida, Hitaru, Yokoyama, Kazumasa, Kuroiwa, Yoshiyuk, Hattori, Nobutaka, Okamoto, Tomoko, Murata, Miho, Toda, Tatsushi, Miyake, Sachiko, Yamamura, Takashi
    PLoS ONE, 2013-12-10;8(12):e83036.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013-02-27;190(7):3696-705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
    Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
    J Transl Med, 2012-07-12;10(0):146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Human TCR gamma delta+ T cells represent a novel target for IL-27 activity.
    Authors: Morandi F, Prigione I, Airoldi I
    Eur. J. Immunol., 2012-06-01;42(6):1547-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010-10-27;185(11):6646-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. CXCR3-B can mediate growth-inhibitory signals in human renal cancer cells by down-regulating the expression of heme oxygenase-1.
    Authors: Datta D, Banerjee P, Gasser M
    J. Biol. Chem., 2010-09-20;285(47):36842-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Phenotypic and functional markers for 1alpha,25-dihydroxyvitamin D(3)-modified regulatory dendritic cells.
    Authors: Pedersen AW, Holmstrom K, Jensen SS, Fuchs D, Rasmussen S, Kvistborg P, Claesson MH, Zocca MB
    Clin. Exp. Immunol., 2009-07-01;157(1):48-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. The chemokine receptor CXCR3 is degraded following internalization and is replenished at the cell surface by de novo synthesis of receptor.
    Authors: Meiser A, Mueller A, Wise EL, McDonagh EM, Petit SJ, Saran N, Clark PC, Williams TJ, Pease JE
    J. Immunol., 2008-05-15;180(10):6713-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007-07-05;82(4):946-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007-02-01;62(2):170-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006-10-11;35(3):207-16.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  21. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
    Authors: D'Ambrosio D
    Eur. J. Immunol., 2002-10-01;32(10):2934-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
    Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
    J. Exp. Med., 2001-11-05;194(9):1375-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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