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Human CXCL10/IP-10/CRG-2 Antibody

Catalog #: MAB266
43 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB266-SP
MAB266-100
MAB266-500
Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody.
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Product Details
Citations (43)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL10/IP-10/CRG-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL10/IP-10/CRG-2 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 33036
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL10/IP-10/CRG-2
Val22-Pro98
Accession # P02778.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin

Human CXCL10/IP-10 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog # BAF266)

Standard: Recombinant Human CXCL10/IP-10 Protein (Catalog # 266-IP)

Neutralization
Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 0.2 µg/mL Recombinant Human CXCL10/IP‑10/CRG‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody. View Larger

Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody. Recombinant Human CXCL10/ IP-10 (Catalog # 266-IP) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/ IP-10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL10/IP-10 Mono-clonal Antibody (Catalog # MAB266). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Citations for Human CXCL10/IP-10/CRG-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
    Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
    Frontiers in Immunology
  2. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer
    Authors: Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al.
    Cancer Immunology Research
  3. Targeting high circDNA2v levels in colorectal cancer induces cellular senescence and elicits an anti-tumor secretome
    Authors: Wu, S;Dai, X;Xia, Y;Zhao, Q;Zhao, H;Shi, Z;Yin, X;Liu, X;Zhang, A;Yao, Z;Zhang, H;Li, Q;Thorne, RF;Zhang, S;Sheng, W;Hu, W;Gu, H;
    Cell reports
    Species: Human
    Sample Types: Transduced Whole Cells
    Applications: Neutralization
  4. Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model
    Authors: Zhenqing Liu, Jianfei Chao, Cheng Wang, Guihua Sun, Daniel Roeth, Wei Liu et al.
    Cell Reports
  5. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses
    Authors: R Ravindran, C McReynolds, J Yang, BD Hammock, A Ikram, A Ali, A Bashir, T Zohra, WLW Chang, DJ Hartigan-O, HH Rashidi, IH Khan
    PLoS ONE, 2021-07-09;16(7):e0254367.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  6. Multiplexed, quantitative serological profiling of COVID-19 from blood by a point-of-care test
    Authors: Jacob T. Heggestad, David S. Kinnamon, Lyra B. Olson, Jason Liu, Garrett Kelly, Simone A. Wall et al.
    Science Advances
  7. Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
    Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
    Cancers, 2021-03-16;13(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  8. Induction of CXCL10-Mediated Cell Migration by Different Types of Galectins
    Authors: DB AbuSamra, N Panjwani, P Argüeso
    Cells, 2021-01-30;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Multiplexed, quantitative serological profiling of COVID-19 from a drop of blood by a point-of-care test
    Authors: JT Heggestad, D Kinnamon, L Olson, J Liu, G Kelly, S Wall, C Fontes, D Joh, A Hucknall, C Pieper, I Naqvi, L Chen, L Que, T Oguin, S Nair, B Sullenger, C Woods, G Sempowski, B Kraft, A Chilkoti
    medRxiv, 2020-11-07;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  10. Human Nasal Turbinate Tissues in Organ Culture as a Model for Human Cytomegalovirus Infection at the Mucosal Entry Site
    Authors: Or Alfi, Ido From, Arkadi Yakirevitch, Michael Drendel, Michael Wolf, Karen Meir et al.
    Journal of Virology
  11. DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer
    Authors: AA de Cubas, W Dunker, A Zaninovich, RA Hongo, A Bhatia, A Panda, KE Beckermann, G Bhanot, S Ganesan, J Karijolich, WK Rathmell
    JCI Insight, 2020-06-04;5(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4
    Authors: Mudan Zhang, Xu Deng, Xinmeng Guan, Lanlan Geng, Ming Fu, Binman Zhang et al.
    Frontiers in Immunology
  13. Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice
    Authors: S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino
    Cell Mol Gastroenterol Hepatol, 2018-09-11;7(1):115-134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection, Neutralization
  14. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016-11-28;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016-07-25;6(0):29909.
    Species: Human
    Sample Types: Serum
  16. Escherichia coli Pyruvate Dehydrogenase Complex Is an Important Component of CXCL10-Mediated Antimicrobial Activity.
    Authors: Schutte K, Fisher D, Burdick M, Mehrad B, Mathers A, Mann B, Nakamoto R, Hughes M
    Infect Immun, 2015-11-09;84(1):320-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Immunoassay Development
  17. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014-02-22;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014-02-14;133(4):1227-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  19. Human rhinovirus-induced ISG15 selectively modulates epithelial antiviral immunity.
    Authors: Zaheer R, Wiehler S, Hudy M, Traves S, Pelikan J, Leigh R, Proud D
    Mucosal Immunol, 2014-01-22;7(5):1127-38.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  20. Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients.
    Authors: Srivastava R, Lee S, Andrade Filho P, Lord C, Jie H, Davidson H, Lopez-Albaitero A, Gibson S, Gooding W, Ferrone S, Ferris R
    Clin Cancer Res, 2013-02-26;19(7):1858-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  21. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012-11-23;26(3):357-69.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012-09-17;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2010-08-19;131(1):133-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2009-10-22;51(2):643-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009-07-28;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  28. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  29. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  30. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007-10-01;179(7):4840-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  31. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007-10-01;13(19):5719-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  32. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007-09-15;179(6):4272-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunodepletion
  33. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  34. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  35. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Blister Fluid, Cell Culture Supernates
    Applications: ELISA Development, Neutralization
  36. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  37. Enhanced type I interferon signalling promotes Th1-biased inflammation in cutaneous lupus erythematosus.
    Authors: Wenzel J, Worenkamper E, Freutel S, Henze S, Haller O, Bieber T, Tuting T
    J. Pathol., 2005-03-01;205(4):435-42.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  38. Simian human immunodeficiency virus-associated pneumonia correlates with increased expression of MCP-1, CXCL10, and viral RNA in the lungs of rhesus macaques.
    Authors: Sui Y, Li S, Pinson D, Adany I, Li Z, Villinger F, Narayan O, Buch S
    Am. J. Pathol., 2005-02-01;166(2):355-65.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  39. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  40. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004-03-25;104(2):314-20.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, ICC
  41. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  42. Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer
    Authors: Jie X, Chen Y, Zhao Y et al.
    Journal for immunotherapy of cancer
  43. Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
    Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
    J. Infect. Dis., 1999-03-01;179(3):646-52.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Types: Plasma
    Applications: ELISA Development

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Reviews for Human CXCL10/IP-10/CRG-2 Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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Human CXCL10/IP-10/CRG-2 Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Primate

Worked as capture antibody

ELISA Human CXCL10/IP-10/CRG-2 Antibody MAB266
Human CXCL10/IP-10/CRG-2 Antibody
By Anonymous on 08/17/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human
ELISA Human CXCL10/IP-10/CRG-2 Antibody MAB266
Human CXCL10/IP-10/CRG-2 Antibody
By Anonymous on 07/13/2018
Application: ELISA Sample Tested: Plasma Species: Human
ELISA Human CXCL10/IP-10/CRG-2 Antibody MAB266
Human CXCL10/IP-10/CRG-2 Antibody
By Anonymous on 11/17/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Sandwich ELISA using MAB266 on solid phase and BAF266 as detection.

ELISA Human CXCL10/IP-10/CRG-2 Antibody MAB266