Human CX3CR1 Fluorescein-conjugated Antibody

Name: Human CX3CR1 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB5204F

Catalog #: FAB5204F
1 Citation Datasheet / COA / SDS

Newer Version Available: FAB5204P

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Discontinued Product

FAB5204F has been discontinued and is replaced by FAB5204P. FAB5204C.

Detection of CX3CR1 in Human Blood Monocytes by Flow Cytometry.

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All CX3CR1 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CX3CR1 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CX3CR1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 528728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CX3CR1
Met1-Thr31, Leu91-Lys103, Thr168-Thr195, Lys257-Leu273
Accession # NP_001328

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CX3CR1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CX3CR1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5204F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 is a 40 kDa seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia, and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion co-receptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.

Long Name

CX3C Chemokine Receptor 1

Entrez Gene IDs

1524 (Human); 13051 (Mouse); 171056 (Rat)

Alternate Names

Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CX3CR1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD.
Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
PLoS ONE, 2014-12-15;9(12):e112473.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry