Home / CX3CL1/Fractalkine / Human CX3CL1/Fractalkine Chemokine Domain Antibody

Human CX3CL1/Fractalkine Chemokine Domain Antibody

Catalog #: MAB365
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB365-SP
MAB365-500
MAB365-100
Product Details
Citations (12)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CX3CL1/Fractalkine Chemokine Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 51637
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
Gln25-Arg339 (Ser199Asn) (predicted)
Accession # P78423
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

References
  1. Pan, Y. et al. (1997) Nature 387:611.
  2. Bazan, J.F. et al. (1997) Nature 385:640.
  3. Mackay, C.R. (1997) Current Biology 7:R384.
Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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Citations for Human CX3CL1/Fractalkine Chemokine Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Neural Crest Cells Isolated from the Bone Marrow of Transgenic Mice Express JCV T-Antigen
    Authors: Jennifer Gordon, Ilker K. Sariyer, Marisol De La Fuente-Granada, Brian J. Augelli, Jessica Otte, S. Ausim Azizi et al.
    PLoS ONE
  2. CX3CL1(+) Microparticles-Induced MFG-E8 Enhances Apoptotic Cell Clearance by Alveolar Macrophages
    Authors: WH Tsai, SC Chang, YC Lin, HC Hsu
    Cells, 2021-09-28;10(10):.
    Species: Human
    Sample Types: Microparticles
    Applications: Flow Cytometry
  3. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
    Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
    Mediators Inflamm., 2017-03-26;2017(0):1515389.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
    Authors: Suzuki F, Kubota T
    Arthritis Res. Ther., 2012-03-06;14(2):R48.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
    Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
    Blood, 2008-09-17;112(13):5026-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
    Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
    J. Immunol., 2007-01-15;178(2):1172-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell-based ELISA
  7. The uremic solute p-cresol decreases leukocyte transendothelial migration in vitro.
    Authors: Faure V, Cerini C, Paul P, Berland Y, Dignat-George F, Brunet P
    Int. Immunol., 2006-09-05;18(10):1453-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
    Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
    Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  9. Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth.
    Authors: Chazaud B, Sonnet C, Lafuste P, Bassez G, Rimaniol AC, Poron F, Authier FJ, Dreyfus PA, Gherardi RK
    J. Cell Biol., 2003-12-08;163(5):1133-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.
    Authors: Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR
    Am. J. Pathol., 2001-03-01;158(3):855-66.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  11. Integrins alpha v beta 3 and alpha 4 beta 1 Act as Coreceptors for Fractalkine, and the Integrin-Binding Defective Mutant of Fractalkine Is an Antagonist of CX3CR1
    Authors: Masaaki Fujita, Yoko K. Takada, Yoshikazu Takada
    The Journal of Immunology
  12. CX3CL1(+) Microparticles Mediate the Chemoattraction of Alveolar Macrophages toward Apoptotic Acute Promyelocytic Leukemic Cells
    Authors: Wen-Hui Tsai, Chung-Hung Shih, Shan-Yu Feng, I-Ting Li, Shao-Chi Chang, Yu-Chieh Lin et al.
    Cellular Physiology and Biochemistry

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