Human CX3CL1/Fractalkine Chemokine Domain Antibody

Catalog #: MAB365
12 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB365-SP
MAB365-500
MAB365-100

Product Details

Citations (12)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CX3CL1/Fractalkine Chemokine Domain Antibody Summary

Species Reactivity

Human

Specificity

Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 51637

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
Gln25-Arg339 (Ser199Asn) (predicted)
Accession # P78423

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

References

Pan, Y. et al. (1997) Nature 387:611.
Bazan, J.F. et al. (1997) Nature 385:640.
Mackay, C.R. (1997) Current Biology 7:R384.

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Alternate Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CX3CL1/Fractalkine Chemokine Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Neural Crest Cells Isolated from the Bone Marrow of Transgenic Mice Express JCV T-Antigen
Authors: Jennifer Gordon, Ilker K. Sariyer, Marisol De La Fuente-Granada, Brian J. Augelli, Jessica Otte, S. Ausim Azizi et al.
PLoS ONE
CX3CL1(+) Microparticles-Induced MFG-E8 Enhances Apoptotic Cell Clearance by Alveolar Macrophages
Authors: WH Tsai, SC Chang, YC Lin, HC Hsu
Cells, 2021-09-28;10(10):.
Species: Human
Sample Types: Microparticles
Applications: Flow Cytometry
Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
Mediators Inflamm., 2017-03-26;2017(0):1515389.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
Authors: Suzuki F, Kubota T
Arthritis Res. Ther., 2012-03-06;14(2):R48.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
Blood, 2008-09-17;112(13):5026-36.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
J. Immunol., 2007-01-15;178(2):1172-9.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA
The uremic solute p-cresol decreases leukocyte transendothelial migration in vitro.
Authors: Faure V, Cerini C, Paul P, Berland Y, Dignat-George F, Brunet P
Int. Immunol., 2006-09-05;18(10):1453-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
Eur. J. Neurosci., 2004-12-01;20(12):3222-32.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr
Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth.
Authors: Chazaud B, Sonnet C, Lafuste P, Bassez G, Rimaniol AC, Poron F, Authier FJ, Dreyfus PA, Gherardi RK
J. Cell Biol., 2003-12-08;163(5):1133-43.
Species: Human
Sample Types: Whole Cells
Applications: ICC
The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.
Authors: Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR
Am. J. Pathol., 2001-03-01;158(3):855-66.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot

Integrins alpha v beta 3 and alpha 4 beta 1 Act as Coreceptors for Fractalkine, and the Integrin-Binding Defective Mutant of Fractalkine Is an Antagonist of CX3CR1
Authors: Masaaki Fujita, Yoko K. Takada, Yoshikazu Takada
The Journal of Immunology

CX3CL1(+) Microparticles Mediate the Chemoattraction of Alveolar Macrophages toward Apoptotic Acute Promyelocytic Leukemic Cells
Authors: Wen-Hui Tsai, Chung-Hung Shih, Shan-Yu Feng, I-Ting Li, Shao-Chi Chang, Yu-Chieh Lin et al.
Cellular Physiology and Biochemistry