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Human Collagen XXIII  alpha 1 Antibody

Catalog #: MAB4165
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4165
MAB4165-SP
Detection of Human Collagen XXIII alpha 1 by Western Blot.
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Citations (3)
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Human Collagen XXIII  alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Collagen XXIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50% cross-reactivity with recombinant human Collagen XXV alpha 1 is observed.
Source
Monoclonal Mouse IgG2b Clone # 468642
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Collagen XXIII a1 antibody by Western Blot. View Larger

Detection of Human Collagen XXIII alpha 1 by Western Blot. Western blot shows lysates of NCI-H460 human large cell lung carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Collagen XXIII a1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Collagen XXIII a1 antibody in Human Adenocarcinoma by Immunohistochemistry (IHC-P). View Larger

Collagen XXIII alpha 1 in Human Adenocarcinoma. Collagen XXIII a1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Human Collagen XXIII a1 Monoclonal Antibody (Catalog # MAB4165) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Collagen XXIII alpha 1

Collagen XXIII alpha 1 (COL23A1) is an epithelial cell type 2 transmembrane glycoprotein with multiple collagen like domains in its extracellular region. Intracellular furin mediated cleavage of the 68 kDa molecule generates a 58 kDa shed fragment that forms disulfide linked trimers. An alternately spliced isoform consists of nearly 300 amino acids of the membrane proximal extracellular domain (ECD), flanked by substituted sequences. COL23A1 is upregulated in many transformed cell lines and serves as an indicator for tumor aggressiveness in prostate cancer. Within the ECD, human COL23A1 shares 89% aa sequence identity with mouse and rat COL23A1.

Entrez Gene IDs
91522 (Human); 237759 (Mouse); 353303 (Rat)
Alternate Names
COL23A1; collagen alpha-1(XXIII) chain; Collagen XXIII alpha 1; collagen, type XXIII, alpha 1; DKFZp434K0621; procollagen, type XXIII, alpha 1

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Citations for Human Collagen XXIII  alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The Oncogenic Role of COL23A1 in Clear Cell Renal Cell Carcinoma
    Authors: Fujiang Xu, Kun Chang, Jian Ma, Yuanyuan Qu, Huyang Xie, Bo Dai et al.
    Scientific Reports
  2. Elevated ectodomain of type 23 collagen is a novel biomarker of the intestinal epithelium to monitor disease activity in ulcerative colitis and Crohn's disease
    Authors: T. Manon‐Jensen, S. Sun, M. Lindholm, V. Domislović, P. Giuffrida, M. Brinar et al.
    United European Gastroenterology Journal
  3. A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis.
    Authors: Spivey KA, Chung I, Banyard J
    Oncogene, 2011-10-03;31(18):2362-72.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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