Human CLEC14A Antibody Summary
Glu22-Ala397
Accession # Q86T13
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Clec-14A in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
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CLEC14A in HUVEC Human Cells. CLEC14A was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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CLEC14A in Human Breast Cancer Tissue. CLEC14A was detected in formalin fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human CLEC14A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4968) at 1.7 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Universal (Catalog # CTS015). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC14A
CLEC14A (C-type lectin domain family 14 member A; also EGFR5) is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. It is a type I transmembrane protein, apparently expressed in brain and about which little is known. Mature human CLEC14A is 469 amino acids in length. It contains a 376 aa extracellular region (aa 22-397) and a 72 aa cytoplasmic domain. The extracellular region shows one C-type lectin like domain (aa 32-175) and an EGF-like region (aa 245-287). Over aa 22-397, human CLEC14A shares 66% and 81% aa identity with mouse and canine CLEC14A, respectively.
Product Datasheets
Citations for Human CLEC14A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth
Authors: X Zhuang, F Maione, J Robinson, M Bentley, B Kaul, K Whitworth, N Jumbu, E Jinks, J Bystrom, P Gabriele, E Garibaldi, E Delmastro, Z Nagy, D Gilham, E Giraudo, R Bicknell, SP Lee
JCI Insight, 2020-10-02;5(19):.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
An evaluation of the tumour endothelial marker CLEC14A as a therapeutic target in solid tumours
Authors: J Robinson, K Whitworth, E Jinks, Z Nagy, R Bicknell, SP Lee
J Pathol Clin Res, 2020-07-21;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of VEGF-dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC14a
Authors: TK Kim, CS Park, J Jang, MR Kim, HJ Na, K Lee, HJ Kim, K Heo, BC Yoo, YM Kim, JW Lee, SJ Kim, ES Kim, DY Kim, K Cha, TG Lee, S Lee
Mol Oncol, 2018-01-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CLEC14a-HSP70-1A interaction regulates HSP70-1A-induced angiogenesis
Authors: J Jang, MR Kim, TK Kim, WR Lee, JH Kim, K Heo, S Lee
Sci Rep, 2017-09-06;7(1):10666.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2).
Authors: Noy P, Swain R, Khan K, Lodhia P, Bicknell R
FASEB J, 2016-03-03;30(6):2311-23.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth.
Authors: Noy P, Lodhia P, Khan K, Zhuang X, Ward D, Verissimo A, Bacon A, Bicknell R
Oncogene, 2015-03-09;34(47):5821-31.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro.
Authors: Ki, M K, Jeoung, M H, Choi, J R, Rho, S-S, Kwon, Y-G, Shim, H, Chung, J, Hong, H J, Song, B D, Lee, S
Oncogene, 2013-05-06;32(48):5449-57.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification and angiogenic role of the novel tumor endothelial marker CLEC14A.
Authors: Mura M, Swain RK, Zhuang X
Oncogene, 2011-06-27;31(3):293-305.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P, Neutralization
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