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Human CHRM3 Antibody

Catalog #: MAB6378 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6378
MAB6378-SP
Detection of CHRM3 in HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP by Flow Cytometry.
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Human CHRM3 Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA
Source
Monoclonal Mouse IgG2B Clone # 580011
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CHRM3
Accession # P20309
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cortex) tissue
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CHRM3 antibody in HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP antibody by Flow Cytometry. View Larger

Detection of CHRM3 in HEK293 Human Cell Line Transfected with Human CHRM3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CHRM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry CHRM3 antibody in Human Brain (Cortex) Tissue by Immunohistochemistry (IHC-P). View Larger

CHRM3 in Human Brain (Cortex) Tissue. CHRM3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) tissue using Mouse Anti-Human CHRM3 Monoclonal Antibody (Catalog # MAB6378) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CHRM3

Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.

Long Name
Cholinergic Receptor Muscarinic 3
Entrez Gene IDs
1131 (Human); 12671 (Mouse); 24260 (Rat)
Alternate Names
cholinergic receptor, muscarinic 3; CHRM3; HM3; m3 muscarinic receptor; mAChR M3; muscarinic acetylcholine receptor M3

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