Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Chemerin Quantikine ELISA Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
7.8 pg/mL
Assay Range
78.0 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Chemerin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Chemerin Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Chemerin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Chemerin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Chemerin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 513 | 1558 | 3016 | 515 | 1592 | 3147 |
| Standard Deviation | 23.3 | 66.5 | 84.6 | 40.5 | 121 | 200 |
| CV% | 4.5 | 4.3 | 2.8 | 7.9 | 7.6 | 6.4 |
Recovery
The recovery of Chemerin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 104 | 94-111 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Chemerin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chemerin
Chemerin, also known as Tazarotene-induced Gene 2, (Tig-2), is a member of the Cystatin superfamily. Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin fold-containing domain, and a six aa C-terminal prosegment. It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades. Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.
Long Name:
Retinoic Acid Receptor Responder Protein 2
Entrez Gene IDs:
5919 (Human); 71660 (Mouse)
Alternate Names:
Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Chemerin Quantikine ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Adipocytokines levels as potential biomarkers for discriminating patients with a diagnosis of depressive disorder from healthy controls
Authors: Ma?uj?o-Balcerska, E;Pietras, T;
Journal of psychiatric research
Species: Human
Sample Types: Serum
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Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment
Authors: K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat
International Journal of Molecular Sciences, 2023-01-09;24(2):.
Species: Human
Sample Types: Plasma
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Analysis of an Indian diabetes prevention programme on association of adipokines and a hepatokine with incident diabetes
Authors: P Susairaj, C Snehalatha, A Nanditha, K Satheesh, A Raghavan, R Vinitha, A Ramachandr
Scientific Reports, 2021-10-13;11(1):20327.
Species: Human
Sample Types: Serum
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Serum chemerin levels: A potential biomarker of joint inflammation in women with rheumatoid arthritis
Authors: F Gonzalez-P, JI Gamez-Nava, EE Perez-Guer, AM Saldaña-Cr, ML Vazquez-Vi, JM Ponce-Guar, M Huerta, X Trujillo, B Contreras-, AD Rocha-Muño, MO Carrillo-E, EN Sanchez-Ro, EE Gomez-Rami, CA Nava-Valdi, EG Cardona-Mu, L Gonzalez-L, Research G
PLoS ONE, 2021-09-10;16(9):e0255854.
Species: Human
Sample Types: Serum
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Clinical Significance of Serum Adipokines according to Body Mass Index in Patients with Clinically Localized Prostate Cancer Undergoing Radical Prostatectomy
Authors: M Kang, SS Byun, SE Lee, SK Hong
World J Mens Health, 2018-01-01;36(1):57-65.
Species: Human
Sample Types: Serum
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Mesenchymal stromal cell-secreted chemerin is a novel immunomodulatory molecule driving the migration of ChemR23-expressing cells
Authors: Paola Vinci
Cytotherapy, 2016-12-06;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
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Reviews for Human Chemerin Quantikine ELISA
Average Rating: 5 (Based on 2 Reviews)
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Human Chemerin Quantikine ELISA
By Anonymous
on 10/01/2019
Sample Tested: Human serum
Led to publishable results
Human Chemerin Quantikine ELISA
By Diego Rodríguez-Penas
on 07/12/2016
Sample Tested: EDTA Plasma
