Human CEACAM-8/CD66b Antibody

Catalog #: MAB4246
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB4246
MAB4246-SP

Detection of Human CEACAM‑8/CD66b by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEACAM-8/CD66b Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-8/CD66b in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 913542

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E.coli-derived recombinant human CEACAM-8/CD66b
Gln35-His141
Accession # P31997

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CEACAM‑8/CD66b by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) and human peripheral blood granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-8/CD66b

CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8), also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human
CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CEACAM-8/CD66b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1