Human CD79A Antibody

Catalog #: MAB69201 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB69201
MAB69201-SP

Detection of CD79A in Human Blood Monocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD79A Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD79A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD79A is observed.

Source

Monoclonal Mouse IgG₁ Clone # 706931

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD79A
Leu33-Arg143
Accession # P11912

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood monocytes treated with Recombinant Human IL-2 (Catalog # 202-IL) and PBMC with CD19 costain

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD79A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with Recombinant Human IL-2 (202-IL) were stained with Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB69201) Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). Quadrant markers were set based on control antibody staining (MAB002).

Flow Cytometry

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Detection of CD79A in PBMC with CD19 costain cells by Flow Cytometry. PBMC with CD19 costain were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB69201) or (B) Mouse IgG₁ Phycoerythrin Isotype Control (Catalog # IC002P) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD79A

CD79A, also known as Ig alpha and MB-1, is a 44 kDa transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig-like domain in its extracellular region (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform with a 39 aa deletion in the ECD. Heterodimers of CD79A and CD79B/Ig beta associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD, human CD79A shares 57% aa sequence identity with mouse and rat CD79A.

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat); 722190 (Rhesus Macaque)

Alternate Names

CD79A antigen (immunoglobulin-associated alpha); CD79a antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A; IGAB-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; MB1; MB-1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein