Human CD69 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB23591P
Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (5)
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Human CD69 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.
Source
Monoclonal Mouse IgG2A Clone # 298614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
Human peripheral blood lymphocytes; PBMCs treated with 5 ug/mL PHA for 2 days or left untreated

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD69 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB23591P) or isotype control antibody (IC003P, data not shown). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CD69 in stimulated vs. naive PBMCs by Flow Cytometry. PBMCs either treated with 5 ug/mL PHA for 2 days (A) or (B) left untreated (naive) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human CD69 PE‑conjugated Monoclonal Antibody (Catalog # FAB23591P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

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Citations for Human CD69 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Role of natural killer cells in liver transplantation treatment of liver cancer
    Authors: Wenbin Ji, Jin Chen, Yuche Mi, Guiliang Wang, Xinjiang Xu, Weizheng Wang
    Experimental and Therapeutic Medicine
  2. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011-05-19;6(5):e19988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009-08-08;133(2):257-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function
    Authors: Qingqu Guo, Yiming Chu, Hongbo Li, Dike Shi, Lele Lin, Weifeng Lan et al.
    European Journal of Medical Research

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