Human CD69 APC-conjugated Antibody

Catalog #: FAB23591A Datasheet / COA / SDS

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FAB23591A

Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD69 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 298614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD69
Gly64-Lys199
Accession # Q07108

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD69 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either untreated (light orange filled histogram) or treated with PHA (dark orange filled histogram) were stained with Mouse Anti-Human CD69 APC-conjugated Monoclonal Antibody (Catalog # FAB23591A) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Entrez Gene IDs

969 (Human); 12515 (Mouse)

Alternate Names

activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60