Human CD59 Antibody Summary
Leu26-Asn102
Accession # P13987
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human CD59 by Western Blot. Western blot shows lysates of human breast tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD59 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
CD59 in Human Breast. CD59 was detected in immersion fixed paraffin-embedded sections of human breast using 1.7 µg/mL Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
View Larger
Detection of Human CD59 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for CD59 at approximately 26 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD59
CD59 is a GPI-anchored membrane glycoprotein that functions to inhibit formation of the complement membrane attack complex and thereby prevent cell injury. CD59 is expressed on erythrocytes and on all peripheral blood leukocytes.
Product Datasheets
Citations for Human CD59 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Using chemiluminescence imaging of cells (CLIC) for relative protein quantification
Authors: J Fisher, OE Sørensen, AHA Abu-Humaid
Sci Rep, 2020-10-26;10(1):18280.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Apolipoprotein J/Clusterin in Human Erythrocytes Is Involved in the Molecular Process of Defected Material Disposal during Vesiculation.
Authors: Antonelou MH, Kriebardis AG, Stamoulis KE
PLoS ONE, 2011-10-07;6(10):e26033.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Angiotensin II controls occludin function and is required for blood brain barrier maintenance: relevance to multiple sclerosis.
Authors: Wosik K, Cayrol R, Dodelet-Devillers A, Berthelet F, Bernard M, Moumdjian R, Bouthillier A, Reudelhuber TL, Prat A
J. Neurosci., 2007-08-22;27(34):9032-42.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Differences in Extracellular Vesicle Protein Cargo Are Dependent on Head and Neck Squamous Cell Carcinoma Cell of Origin and Human Papillomavirus Status.
Authors: Goudsmit, C, da Veiga Leprevost, F, et al.
Cancers (Basel)
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human CD59 Antibody
There are currently no reviews for this product. Be the first to review Human CD59 Antibody and earn rewards!
Have you used Human CD59 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
