Human CD45RB Antibody

Catalog # Availability Size / Price Qty
MAB10477-100
MAB10477-SP
Detection of CD45RB in Human Lymph Node.
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Human CD45RB Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD45RB in direct ELISA.
Source
Monoclonal Mouse IgG1 Clone # 1067419
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human CD45RB
Gln26-Lys463
Accession # P08575
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of Human Lymph Node

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of CD45RB in Human Lymph Node. CD45RB was detected in immersion fixed paraffin-embedded sections of Human Lymph Node using Mouse Anti-Human CD45RB Monoclonal Antibody (Catalog # MAB10477) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD45RB

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes and the shortest form, CD45R0 is expressed on memory cells (5). Isoform CD45RB contains exon 5 (B exon) in the extracellular domain, which shares 41% and 40% homology with the ECD of mouse and rat CD45RB. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Interaction of galaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates of CD45 (4, 9). Within the immunological synapse, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
CD45R, B isoform
Entrez Gene IDs
5788 (Human); 19264 (Mouse)
Alternate Names
B220; CD45 antigen; CD45R; CD45RB; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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