Home / CD45 / Human CD45 Phycoerythrin MAb (Clone 2D1)

Human CD45 Phycoerythrin MAb (Clone 2D1)

Catalog #: FAB1430P
20 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1430P-100
FAB1430P-025
Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (20)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD45 Phycoerythrin MAb (Clone 2D1) Summary

Species Reactivity
Human
Specificity
Detects human CD45. This antibody recognizes all isoforms of human CD45.
Source
Monoclonal Mouse IgG1 Clone # 2D1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human peripheral blood mononuclear cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD45 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB1430P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human CD45 by Flow Cytometry View Larger

Detection of Human CD45 by Flow Cytometry Isolation and characterization of amniotic derived mesenchymal stem cells with renal marker expression(A) Light microscopic image of mesenchymal stem cells isolated from amniotic fluid. A subpopulation of these cells (with renal markers) displays a trumpet shaped phenotype (scale bar = 200 μm). (B) hAFSCs were characterized by flow cytometry (positive for CD24, CD117, CD73, HLA-ABC, CD29, and CD105 but not for CD45, or CD34). (C) Confocal imaging of hAFSCs for renal markers SIX2 and PAX2. (D) Expression of Six2 and Pax2 (left panel) in hAFSCs compared to human embryonic kidney (hEK). Agarose gel analysis of PCR fragments (SIX2: 336 bp; PAX2: 65 bp; KSP: 152bp; GAPDH: 189 bp) (E). hAFSCs were differentiated along adipogenic, osteogenic and chondrogenic lineages (left to right). In the adipogenic differentiation the cells formed lipid vesicles stained with oil red O (left) (magnification 100X). Calcium deposits stained with Alizarin confirmed osteogenesis (center). Micromasses stained with alcian blue confirmed chondrogenesis (right) (scale bar = 200 μm). Quantification of chondrogenic and osteogenic differentiation using dye extraction and quantification with spectrophotometry before and after differentiation (left panel). qPCR analysis of chondrogenic markers (Sox9, Col II) and osteogenic markers (ALP, Runx2 and OST) before and after differentiation (right panel). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0136145), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD45

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the class 1 (Receptor‑like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the Immunological Synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citations for Human CD45 Phycoerythrin MAb (Clone 2D1)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Isolation and Characterization of Multipotent CD24+ Cells From the Renal Papilla of Swine
    Authors: David M. Burmeister, Matthew K. McIntyre, Robbie K. Montgomery, Belinda I. Gómez, Michael A. Dubick
    Frontiers in Medicine
  2. Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
    Authors: Iris A. Otto, Paulina Nuñez Bernal, Margot Rikkers, Mattie H.P. van Rijen, Anneloes Mensinga, Moshe Kon et al.
    iScience
  3. Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/ beta -catenin Signaling Pathway in Dermal Fibroblasts
    Authors: Cong Li, Yu An, Yu Sun, Fan Yang, Quanchen Xu, Zhiguo Wang
    Stem Cell Reviews and Reports
  4. Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro*
    Authors: Letícia Martins SANTOS, Patricia e Silva CARDOSO, Elisa Abreu DINIZ, Juliana Garuba RAHHAL, Carla Renata SIPERT
    Journal of Applied Oral Science
  5. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.
    Authors: Lin J. R, Izar B, et al.
    Elife
  6. Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma
    Authors: Gregory C. Wilson, Sameer H. Patel, Jiang Wang, Kui Xu, Kevin M. Turner, Katrin Anne Becker et al.
    Journal of Molecular Medicine
  7. Human Wharton's Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses
    Authors: MAA Hussein, HAM Hussein, AA Thabet, KM Selim, MA Dawood, AM El-Adly, AA Wardany, A Sobhy, S Magdeldin, A Osama, AM Anwar, M Abdel-Waha, H Askar, EK Bakhiet, S Sultan, AA Ezzat, U Abdel Raou, MM Afifi
    Cells, 2022-04-21;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CD44v6 Defines a New Population of Circulating Tumor Cells Not Expressing EpCAM
    Authors: G Belthier, Z Homayed, F Grillet, C Duperray, J Vendrell, I Krol, S Bravo, JC Boyer, O Villeronce, J Vitre-Boub, D Heaug-Wane, F Macari-Fin, J Smith, M Merlot, G Lossaint, T Mazard, F Portales, J Solassol, M Ychou, N Aceto, E Mamessier, F Bertucci, JM Pascussi, E Samalin, F Hollande, J Pannequin
    Cancers, 2021-10-02;13(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation
    Authors: J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu
    BMC Oral Health, 2021-09-24;21(1):470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma
    Authors: L Jerby-Arno, C Neftel, ME Shore, HR Weisman, ND Mathewson, MJ McBride, B Haas, B Izar, A Volorio, G Boulay, L Cironi, AR Richman, LC Broye, JM Gurski, CC Luo, R Mylvaganam, L Nguyen, S Mei, JC Melms, C Georgescu, O Cohen, JE Buendia-Bu, A Segerstolp, M Sud, MS Cuoco, D Labes, S Gritsch, DR Zollinger, N Ortogero, JM Beechem, G Petur Niel, I Chebib, T Nguyen-Ngo, M Montemurro, GM Cote, E Choy, I Letovanec, S Cherix, N Wagle, PK Sorger, AB Haynes, JT Mullen, I Stamenkovi, MN Rivera, C Kadoch, KW Wucherpfen, O Rozenblatt, ML Suvà, N Riggi, A Regev
    Nature Medicine, 2021-01-25;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Membranes derived from human umbilical cord Wharton's jelly stem cells as novel bioengineered tissue-like constructs
    Authors: BD Jaimes-Par, I Garzón, V Carriel, D Durand-Her, MA Martín-Pie, JM García, MC Sánchez-Qu, M Alaminos, A Campos
    Histol. Histopathol., 2017-04-26;0(0):11897.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016-08-25;11(8):e0160153.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats.
    Authors: Monteiro Carvalho Mori da M, Zia S, Oliveira Arcolino F, Carlon M, Beckmann D, Pippi N, Luhers Graca D, Levtchenko E, Deprest J, Toelen J
    PLoS ONE, 2015-08-21;10(8):e0136145.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Impaired T cell responsiveness to interleukin-6 in hematological patients with invasive aspergillosis.
    Authors: Camargo J, Bhimji A, Kumar D, Kaul R, Pavan R, Schuh A, Seftel M, Lipton J, Gupta V, Humar A, Husain S
    PLoS ONE, 2015-04-02;10(4):e0123171.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2013-12-04;23(5):541-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Wharton's jelly stem cells: a novel cell source for oral mucosa and skin epithelia regeneration.
    Authors: Garzon I, Miyake J, Gonzalez-Andrades M, Carmona R, Carda C, Sanchez-Quevedo M, Campos A, Alaminos M
    Stem Cells Transl Med, 2013-07-01;2(8):625-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. CCR4-expressing T cell tumors can be specifically controlled via delivery of toxins to chemokine receptors.
    Authors: Baatar D, Olkhanud P, Newton D, Sumitomo K, Biragyn A
    J. Immunol., 2007-08-01;179(3):1996-2004.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Differentiation of human umbilical cord mesenchymal stem cells into steroidogenic cells in vitro
    Authors: Xiaoyu Xing, Zhiyuan Zhang, Liang Zhong, Guanqun Ju, Xiangyu Zou, Yingjian Zhu et al.
    Experimental and Therapeutic Medicine
  20. Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging
    Authors: Ziming Du, Jia-Ren Lin, Rumana Rashid, Zoltan Maliga, Shu Wang, Jon C. Aster et al.
    Nature Protocols

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Human CD45 PE-conjugated Antibody
By Anonymous on 08/16/2016
Application: Flow Sample Tested: Human embryonic stem cells (H9 from Wicell) Species: Human