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Human CD44v6 Antibody

Catalog #: BBA13
31 Citations 1 Review Datasheet / COA / SDS
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BBA13
Detection of CD44 in Human Blood Monocytes by Flow Cytometry.
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Citations (31)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD44v6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD44v6 in Western blots. By flow cytometry analysis using a panel of CD44-transfected COS cells (9), monoclonal antibody clone 2F10 was deduced to be specific for human CD44 protein isoforms containing variant exon 6 (CD44v6).
Source
Monoclonal Mouse IgG1 Clone # 2F10
Purification
Protein A or G purified from ascites
Immunogen
Recombinant human CD44 v3-10
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant HumanCD44 v3-10
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunoprecipitation
Fox, S.B. et al. (1994) Cancer Res. 54:4539.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD44 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CD44 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD44 v6 Monoclonal Antibody (Catalog # BBA13, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).

Immunocytochemistry View Larger

CD44 in A431 Human Cell Line. CD44 was detected in immersion fixed A431 human epithelial carcinoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human CD44 v6 Monoclonal Antibody (Catalog # BBA13) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD44 antibody in Human Colon Adenocarcinoma Tissue by Immunohistochemistry (IHC-P). View Larger

CD44 in Human Colon Adenocarcinoma Tissue. CD44 was detected in immersion fixed paraffin-embedded sections of human colon adenocarcinoma tissue using Mouse Anti-Human CD44 v6 Monoclonal Antibody (Catalog # BBA13) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Detection of Human CD44 by Immunohistochemistry View Larger

Detection of Human CD44 by Immunohistochemistry Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, and TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CD44 by Immunohistochemistry View Larger

Detection of Human CD44 by Immunohistochemistry Immunochemical staining of selected biomarkers in early gastric cancer tissues.(A) E-cadherin (B) a1 catenin (C) p53 (D) EZH (E) CD44v6. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0159424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human CD44 by Western Blot View Larger

Detection of Human CD44 by Western Blot Knockdown of CD44v6 with siRNA transfection in YCC-2 cell line and overexpression of CD44v6 with plasmid transfection in MKN-28 cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0159424), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1‑3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan‑binding disulfide‑stabilized link region and a 325‑530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1‑10, exons 6‑15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). CD44v6 contains exon 10 and is associated with tumor progression and metastasis in many types of cancer including breast, colon, lung, renal, skin, and ovarian tumors. With variable N‑ and O‑glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21‑220), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co‑receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane‑type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase‑dependent intramembrane cleavage to form an A beta ‑like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

References
  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  7. Murakami, D. et al. (2003) Oncogene 22:1511.
  8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
  9. Fox, S.B. et al. (1994) Cancer Res. 54:4539.
Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

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Citations for Human CD44v6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Characterisation of mesenchymal colon tumour-derived cells in tumourspheres as a model for colorectal cancer progression
    Authors: Mimmo Turano, Valeria Costabile, Andrea Cerasuolo, Francesca Duraturo, Raffaella Liccardo, Paolo Delrio et al.
    International Journal of Oncology
  2. NANOG modulates stemness in human colorectal cancer
    Authors: Jingyu Zhang, Luis A. Espinoza, Robert J. Kinders, Scott M. Lawrence, Thomas D. Pfister, Ming Zhou et al.
    Oncogene
  3. Interplay Between Chemotherapy-Activated Cancer Associated Fibroblasts and Cancer Initiating Cells Expressing CD44v6 Promotes Colon Cancer Resistance
    Authors: Shibnath Ghatak, Vincent C. Hascall, Nikos Karamanos, Roger R. Markwald, Suniti Misra
    Frontiers in Oncology
  4. An Orthotopic Patient-Derived Xenograft (PDX) Model Allows the Analysis of Metastasis-Associated Features in Colorectal Cancer
    Authors: De Angelis ML, Francescangeli F, Nicolazzo C et al.
    Frontiers in Oncology
  5. Negative regulation of CD44st by miR-138-5p affects the invasive ability of breast cancer cells and patient prognosis after breast cancer surgery
    Authors: FX Jian, PX Bao, WF Li, YH Cui, HG Hong
    BMC Cancer, 2023-03-24;23(1):269.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression
    Authors: ZS Lin, CC Chung, YC Liu, CH Chang, HC Liu, YY Liang, TL Huang, TM Chen, CH Lee, CH Tang, MC Hung, YH Chen
    Elife, 2023-01-09;12(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  7. Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery
    Authors: S Di Franco, P Bianca, DS Sardina, A Turdo, M Gaggianesi, V Veschi, A Nicotra, LR Mangiapane, M Lo Iacono, I Pillitteri, S van Hooff, F Martorana, G Motta, E Gulotta, VL Lentini, E Martorana, ME Fiori, S Vieni, MR Bongiorno, G Giannone, D Giuffrida, L Memeo, L Colarossi, M Mare, P Vigneri, M Todaro, R De Maria, JP Medema, G Stassi
    Nature Communications, 2021-08-18;12(1):5006.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  8. Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51
    Authors: G Manic, M Musella, F Corradi, A Sistigu, S Vitale, S Soliman Ab, L Mattiello, E Malacaria, C Galassi, M Signore, M Pallocca, S Scalera, F Goeman, F De Nicola, A Guarracino, R Pennisi, F Antonangel, F Sperati, M Baiocchi, M Biffoni, M Fanciulli, M Maugeri-Sa, A Franchitto, P Pichierri, R De Maria, I Vitale
    Cell Death and Differentiation, 2021-02-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CD44v6 Targeted by miR-193b-5p in the Coding Region Modulates the Migration and Invasion of Breast Cancer Cells
    Authors: S Hu, M Cao, Y He, G Zhang, Y Liu, Y Du, C Yang, F Gao
    J Cancer, 2020-01-01;11(1):260-271.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant
    Authors: V Veschi, LR Mangiapane, A Nicotra, S Di Franco, E Scavo, T Apuzzo, DS Sardina, M Fiori, A Benfante, ML Colorito, G Cocorullo, F Giuliante, C Cipolla, G Pistone, MR Bongiorno, A Rizzo, CM Tate, X Wu, S Rowlinson, LF Stancato, M Todaro, R De Maria, G Stassi
    Oncogene, 2019-10-07;0(0):.
    Species: Human, Xenograft
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  11. PPARdelta mediates the effect of dietary fat in promoting colorectal cancer metastasis
    Authors: D Wang, L Fu, J Wei, Y Xiong, RN DuBois
    Cancer Res., 2019-06-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019-05-02;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  13. CD44 targeted delivery of siRNA by using HA-decorated nanotechnologies for KRAS silencing in cancer treatment
    Authors: A Tirella, K Kloc-Munia, L Good, J Ridden, M Ashford, S Puri, N Tirelli
    Int J Pharm, 2019-02-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  14. Novel antibody reagents for characterization of drug- and tumor microenvironment-induced changes in epithelial-mesenchymal transition and cancer stem cells
    Authors: T Navas, TD Pfister, S Colantonio, A Aziz, L Dieckman, RG Saul, J Kaczmarczy, S Borgel, SY Alcoser, MG Hollingshe, YH Lee, DP Bottaro, T Hiltke, G Whiteley, N Takebe, RJ Kinders, RE Parchment, JE Tomaszewsk, JH Doroshow
    PLoS ONE, 2018-06-21;13(6):e0199361.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC
  15. Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells
    Authors: GY Chiou, TW Yang, CC Huang, CY Tang, JY Yen, MC Tsai, HY Chen, N Fadhilah, CC Lin, YJ Jong
    Sci Rep, 2017-05-19;7(1):2172.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance
    Authors: KA Whelan, PM Chandramou, K Tanaka, M Natsuizaka, M Guha, S Srinivasan, DS Darling, Y Kita, S Natsugoe, JD Winkler, AJ Klein-Szan, RK Amaravadi, NG Avadhani, AK Rustgi, H Nakagawa
    Oncogene, 2017-04-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  17. CD44 mediates the Catch-Bond Activated Rolling of HEPG2 Epithelial Cancer Cells on Hyaluronan
    Authors: Axel Rosenhahn
    Cell Adh Migr, 2017-02-01;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-21;113(49):E7917-E7926.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents
    PLoS ONE, 2016-08-09;11(8):e0160952.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Nomogram Incorporating CD44v6 and Clinicopathological Factors to Predict Lymph Node Metastasis for Early Gastric Cancer
    PLoS ONE, 2016-08-02;11(8):e0159424.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Dynamic regulation of the cancer stem cell compartment by Cripto-1 in colorectal cancer.
    Authors: Francescangeli F, Contavalli P, De Angelis M, Baiocchi M, Gambara G, Pagliuca A, Fiorenzano A, Prezioso C, Boe A, Todaro M, Stassi G, Castro N, Watanabe K, Salomon D, De Maria R, Minchiotti G, Zeuner A
    Cell Death Differ, 2015-03-20;22(10):1700-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Overexpression of progelatinase B/proMMP-9 affects migration regulatory pathways and impairs chronic lymphocytic leukemia cell homing to bone marrow and spleen.
    Authors: Bailon E, Ugarte-Berzal E, Amigo-Jimenez I, Van den Steen P, Opdenakker G, Garcia-Marco J, Garcia-Pardo A
    J Leukoc Biol, 2014-08-01;96(2):185-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Establishment of highly tumorigenic human colorectal cancer cell line (CR4) with properties of putative cancer stem cells.
    Authors: Rowehl, Rebecca, Burke, Stephani, Bialkowska, Agnieszk, Pettet, Donald W, Rowehl, Leahana, Li, Ellen, Antoniou, Eric, Zhang, Yuanhao, Bergamaschi, Roberto, Shroyer, Kenneth, Ojima, Iwao, Botchkina, Galina I
    PLoS ONE, 2014-06-12;9(6):e99091.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Evaluation of protein biomarkers of prostate cancer aggressiveness.
    Authors: Rizzardi A, Rosener N, Koopmeiners J, Isaksson Vogel R, Metzger G, Forster C, Marston L, Tiffany J, McCarthy J, Turley E, Warlick C, Henriksen J, Schmechel S
    BMC Cancer, 2014-04-05;14(0):244.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
    Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
    Cell Stem Cell, 2014-03-06;14(3):342-56.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  26. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
    Authors: Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bauerle T, Wallwiener M, Holland-Letz T, Hofner T, Sprick M, Scharpff M, Marme F, Sinn H, Pantel K, Weichert W, Trumpp A
    Nat Biotechnol, 2013-04-21;31(6):539-44.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
    Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
    Cancer Res., 2003-02-15;63(4):887-92.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Effect of hyaluronan on xenotransplanted breast cancer.
    Authors: Herrera-Gayol A, Jothy S
    Exp. Mol. Pathol., 2002-06-01;72(3):179-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. CD44 is exposed to the extracellular matrix at invasive sites in basal cell carcinomas.
    Authors: Dingemans KP, Ramkema MD, Pals ST
    Lab. Invest., 2002-03-01;82(3):313-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunogold Electron Microscopy
  30. Chemotherapy induces feedback up-regulation of CD44v6 in colorectal cancer initiating cells through beta -catenin/MDR1 signaling to sustain chemoresistance
    Authors: Shibnath Ghatak, Vincent C. Hascall, Nikos Karamanos, Roger R. Markwald, Suniti Misra
    Frontiers in Oncology
  31. Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome
    Authors: Diego M. Marzese, Michelle Liu, Jamie L. Huynh, Hajime Hirose, Nicholas C. Donovan, Kelly T. Huynh et al.
    Pigment Cell & Melanoma Research

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Human CD44v6 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human