Human CD44s DuoSet ELISA

Catalog # Availability Size / Price Qty
DY7045-05
Ancillary Products Available
Human CD44 s ELISA Standard Curve
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Human CD44s DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD44 s. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human CD44 s ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD44

CD44 is a ubiquitously expressed receptor for hyaluronan and exerts control over cell growth, migration, and tumor progression. With variable N and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa. CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. It can function as a co-receptor that modifies activity of receptors including Met and the ErbB family tyrosine kinases. The intracellular domain of CD44 links the plasma membrane to the actin cytoskeleton via the ERM proteins, Ezrin, Radixin and Moesin.

Entrez Gene IDs:
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names:
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

Citations for Human CD44s DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Influence of diabetes mellitus on the pathological profile of aortic stenosis: a sex-based approach
    Authors: Martín-Núñez, E;Goñi-Olóriz, M;Matilla, L;Garaikoetxea, M;Mourino-Alvarez, L;Navarro, A;Fernández-Celis, A;Tamayo, I;Gainza, A;Álvarez, V;Sádaba, R;Barderas, MG;Jover, E;López-Andrés, N;
    Cardiovascular diabetology
    Species: Human
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  2. Comparative proteome analysis identified CD44 as a possible serum marker for docetaxel resistance in castration-resistant prostate cancer
    Authors: D Keresztes, A Csizmarik, N Nagy, O Módos, T Fazekas, T Bracht, B Sitek, K Witzke, M Puhr, S Sevcenco, G Kramer, S Shariat, Z Küronya, L Takács, I Tornyi, J Lázár, B Hadaschik, A Lászik, M Sz?cs, P Nyirády, T Szarvas
    Journal of Cellular and Molecular Medicine, 2021-12-30;0(0):.
    Species: Human
    Sample Types: Serum
  3. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
    Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
    Nature Communications, 2021-06-10;12(1):3543.
    Species: Human
    Sample Types: Exosomes
  4. Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection
    Authors: S Novelli, C Lécuroux, C Goujard, J Reynes, A Villemant, L Blum, A Essat, V Avettand-F, O Launay, JM Molina, C Bourgeois, L Meyer
    EBioMedicine, 2020-11-26;62(0):103129.
    Species: Human
    Sample Types: Plasma

FAQs

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Reviews for Human CD44s DuoSet ELISA

Average Rating: 4.5 (Based on 2 Reviews)

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Human CD44s DuoSet ELISA
By Anonymous on 11/08/2018
Sample Tested: EDTA Plasma

Human CD44s DuoSet ELISA
By Anonymous on 11/01/2016
Sample Tested: uman serum

The kit provided very nice standard curve, low background, and detect CD44 in human serum. We have not compared to the other CD44 ELISA.