Human CD20 (Research Grade Rituximab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9575-100
MAB9575-SP
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Detection of CD20 in HEK293 Human cell line Transfected with Human CD20 and eGFP by Flow Cytometry
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Product Details
Citations (1)
FAQs
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Reviews (1)

Human CD20 (Research Grade Rituximab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human CD20 based on Rituximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Rituximab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu2
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CD20
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD20 antibody in HEK293 Human cell line Transfected with Human CD20 and eGFP antibody by Flow Cytometry View Larger

Detection of CD20 in HEK293 Human cell line Transfected with Human CD20 and eGFP by Flow Cytometry HEK293 Human cell line transfected with (A) Human CD20 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CD20 (Rituximab Biosimilar) Monoclonal Antibody (Catalog # MAB9575) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

Rituximab is a biosimilar directed against the extracellular domain of CD20. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB9630. The biotinylated antibody (catalog number FAB9575B) can be used in flow cytometry or various assay format to measure CD20 expression and can be detected by conjugated streptavidin.

Long Name
Cluster of Differentiation 20
Entrez Gene IDs
931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)
Alternate Names
B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

Product Datasheets

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Citation for Human CD20 (Research Grade Rituximab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. KIR+CD8+ and NKG2A+CD8+ T�cells are distinct innate-like populations in humans
    Authors: SJ Choi, JY Koh, MS Rha, IH Seo, H Lee, S Jeong, SH Park, EC Shin
    Cell Reports, 2023-03-09;42(3):112236.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization

FAQs

  1. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  2. Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    • This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

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Human CD20 (Research Grade Rituximab Biosimilar) Antibody
By Anonymous on 06/16/2020
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human