Human CD160 PE-conjugated Antibody Summary
Ile27-Ser159
Accession # O95971
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD160 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD160 PE-conjugated Monoclonal Antibody (Catalog # FAB6700P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human Human CD160 PE-conjugated Antibody by Flow Cytometry Phenotype of CD8 and CD4 T cells stimulated with either non-ubiquitinated or ubiquitinated constructs.MoDCs were transduced with either the non-ubiquitinated (0x, hatched bar) or ubiquitinated (1x, grey bar) HIV-1 CN54 gag constructs and co-cultured with autologous naïve T cells. After each subsequent week in culture, cells were removed and stained with antibodies to A) CD8 or B) CD4 and the indicated surface markers. Cells were analysed by flow cytometry, and the box plots represent the median with upper and lower quartiles, and whiskers represent the min and max values of 9 donors. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24505475), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
Background: CD160
CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules. It is expressed on select hematopoietic cell types, including CD56dim CD16+ cytotoxic NK cells, CD8+ CD28- effector T cells, δ/ gamma T cells, and restricted CD4+ T cells. It is a receptor for HLA-C molecules, and its engagement induces CD160+ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program. Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181). It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one V‑type Ig-like domain (aa 27-122). CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182. The TM form appears to have a splice variant that lacks aa 25-133. Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.
Product Datasheets
Citations for Human CD160 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
Authors: de Mare-Bredemeijer E, Shi X, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk M, de Jonge J, van der Laan L, Metselaar H, Kwekkeboom J
J Immunol, 2015-07-13;195(4):1838-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
Authors: Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
PLoS ONE, 2014-02-05;9(2):e88327.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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