Human CD155/PVR PE-conjugated Antibody Summary
Gly27-Asn343
Accession # AAH15542
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD155/PVR in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human CD155/PVR PE-conjugated Monoclonal Antibody (Catalog # FAB25301P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD155/PVR in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human CD155/PVR PE-conjugated Monoclonal Antibody (Catalog # FAB25301P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD155/PVR
CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6‑8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.
- Takai, Y. et al. (2003) Cancer Sci. 94:655.
- Mendelsohn, C.L. et al. (1989) Cell 56:855.
- Koike, H. et al. (1990) EMBO J. 9:3217.
- Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
- Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
- Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
- Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
- Lange, R. et al. (2001) Virology 285:218.
Product Datasheets
Citations for Human CD155/PVR PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
Oncology letters
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Combinatorial immunotherapy with gemcitabine and ex vivo-expanded NK cells induces anti-tumor effects in pancreatic cancer
Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Kim, J;Bae, JH;Park, YS;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects
Authors: NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest
Nature, 2023-01-11;613(7945):743-750.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Structural and functional characterization of a monoclonal antibody blocking TIGIT
Authors: Bo-Seong Jeong, Hyemi Nam, Jeewon Lee, Hye-Young Park, Ki Joon Cho, Joong Hyuk Sheen et al.
mAbs
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Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
Oncotarget, 2017-01-24;8(4):5954-5964.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Loss of DNAM-1 ligand expression by acute myeloid leukemia cells renders them resistant to NK cell killing.
Authors: Kearney C, Ramsbottom K, Voskoboinik I, Darcy P, Oliaro J
Oncoimmunology, 2016-06-27;5(8):e1196308.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.
Authors: Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F
Leukemia, 2015-03-27;29(9):1799-810.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The cell surface proteome of human mesenchymal stromal cells.
Authors: Niehage C, Steenblock C, Pursche T, Bornhauser M, Corbeil D, Hoflack B
PLoS ONE, 2011-05-26;6(5):e20399.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cytotoxicity of activated natural killer cells against pediatric solid tumors.
Authors: Cho D, Shook DR, Shimasaki N
Clin. Cancer Res., 2010-06-11;16(15):3901-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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