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Human CCR7 PE-conjugated Antibody

Catalog #: FAB197P
71 Citations 3 Reviews Datasheet / COA / SDS
Clone 150503 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB197P-025
FAB197P-100
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (71)
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Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR7 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR7.
Source
Monoclonal Mouse IgG2A Clone # 150503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR7 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 PE-conjugated Monoclonal Antibody (Catalog # FAB197P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR7 antibody in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Entrez Gene IDs
1236 (Human); 12775 (Mouse); 287673 (Rat)
Alternate Names
BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Citations for Human CCR7 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

71 Citations: Showing 1 - 10
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  1. IFN-alpha with dasatinib broadens the immune repertoire in patients with chronic-phase chronic myeloid leukemia
    Authors: Jani Huuhtanen, Mette Ilander, Bhagwan Yadav, Olli M.J. Dufva, Hanna Lähteenmäki, Tiina Kasanen et al.
    Journal of Clinical Investigation
  2. Rapamycin generates anti-apoptotic human Th1/Tc1 cells via autophagy for induction of xenogeneic GVHD
    Authors: Shoba Amarnath, Francis A. Flomerfelt, Carliann M. Costanzo, Jason E. Foley, Jacopo Mariotti, Daniel M. Konecki et al.
    Autophagy
  3. Reprogramming CD19-Specific T Cells with IL-21 Signaling Can Improve Adoptive Immunotherapy of B-Lineage Malignancies
    Authors: Harjeet Singh, Matthew J. Figliola, Margaret J. Dawson, Helen Huls, Simon Olivares, Kirsten Switzer et al.
    Cancer Research
  4. Metabolic Phenotypes of Response to Vaccination in Humans
    Authors: S Li, NL Sullivan, N Rouphael, T Yu, S Banton, MS Maddur, M McCausland, C Chiu, J Canniff, S Dubey, K Liu, V Tran, T Hagan, S Duraisingh, A Wieland, AK Mehta, JA Whitaker, S Subramania, DP Jones, A Sette, K Vora, A Weinberg, MJ Mulligan, HI Nakaya, M Levin, R Ahmed, B Pulendran
    Cell, 2017-05-11;169(5):862-877.e17.
  5. piggyBac Transposon/Transposase System to Generate CD19-Specific T Cells for the Treatment of B-Lineage Malignancies
    Authors: Pallavi V. Raja Manuri, Matthew H. Wilson, Sourindra N. Maiti, Tiejuan Mi, Harjeet Singh, Simon Olivares et al.
    Human Gene Therapy
  6. Single-cell characterization of anti–LAG-3 and anti–PD-1 combination treatment in patients with melanoma
    Authors: Jani Huuhtanen, Henna He Kasanen, Katriina Peltola, Tapio Lönnberg, Virpi Glumoff, Oscar Brück et al.
    Journal of Clinical Investigation
  7. GSK-J4 Inhibition of KDM6B Histone Demethylase Blocks Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells by Modulating NF-?B Signaling
    Authors: Sadeghi, L;Wright, APH;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. The stimulatory effect of fusobacteria on dendritic cells under aerobic or anaerobic conditions
    Authors: S Koido, S Horiuchi, S Kan, T Bito, Z Ito, K Uchiyama, M Saruta, N Sato, T Ohkusa
    Oncogene, 2022-06-23;12(1):10698.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Ibrutinib Does Not Impact CCR7-Mediated Homeostatic Migration in T-Cells from Chronic Lymphocytic Leukemia Patients
    Authors: T Mateu-Albe, A Marcos-Jim, S Wissmann, J Loscertale, F Terrón, JV Stein, C Muñoz-Call, C Cuesta-Mat
    Cancers, 2022-05-31;14(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Proteogenomic identification of an immunogenic HLA class I neoantigen in mismatch-repair-deficient colorectal cancer tissue
    Authors: T Hirama, S Tokita, M Nakatsugaw, K Murata, Y Nannya, K Matsuo, H Inoko, Y Hirohashi, S Hashimoto, S Ogawa, I Takemasa, N Sato, F Hata, T Kanaseki, T Torigoe
    JCI Insight, 2021-07-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Contribution of Heparan Sulphate Binding in CCL21-Mediated Migration of Breast Cancer Cells
    Authors: I Del Molino, A Meeson, K Cooke, MI Malki, B Barron-Mil, JA Kirby, S Ali
    Cancers, 2021-07-10;13(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease
    Authors: D Kim, G Park, J Huuhtanen, S Lundgren, RK Khajuria, AM Hurtado, C Muñoz-Call, L Cardeñoso, V Gómez-Garc, TH Chen-Liang, S Eldfors, P Ellonen, S Hannula, M Kankainen, O Bruck, A Kreutzman, U Salmenniem, T Lönnberg, A Jerez, M Itälä-Reme, M Myllymäki, MAI Keränen, S Mustjoki
    Nat Commun, 2020-05-07;11(1):2246.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
    Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
    Int J Mol Sci, 2020-04-21;21(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Significance of IL-17A-producing CD8+CD103+ skin resident memory T cells in psoriasis lesion and their possible relationship to clinical course
    Authors: K Kurihara, T Fujiyama, P Phadungsak, T Ito, Y Tokura
    J. Dermatol. Sci., 2019-06-20;95(1):21-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients
    Authors: E Jimenez Ve, YV Chew, L Nicholson, H Burns, P Anderson, HT Chen, L Williams, K Keung, NT Zanjani, S Dervish, E Patrick, XM Wang, S Yi, W Hawthorne, S Alexander, PJ O'Connell, M Hu
    PLoS ONE, 2019-05-22;14(5):e0217163.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  16. Immune cell constitution in bone marrow microenvironment predicts outcome in adult ALL
    Authors: H Hohtari, O Brück, S Blom, R Turkki, M Sinisalo, PE Kovanen, O Kallioniem, T Pellinen, K Porkka, S Mustjoki
    Leukemia, 2019-01-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Increased frequency of CCR7+CD4+ T cells from patients with primary Sj�gren's syndrome: An indicator of disease activity rather than of damage severity
    Authors: C Wu, P Yang, H Liu, W Xiao, L Zhao
    Cytokine, 2018-04-21;110(0):9-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Humoral and cell-mediated immune responses after a booster dose of HBV vaccine in HIV-infected children, adolescents and young adults
    Authors: V Giacomet, M Masetti, P Nannini, F Forlanini, M Clerici, GV Zuccotti, D Trabattoni
    PLoS ONE, 2018-02-14;13(2):e0192638.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Phase I clinical trial of a novel autologous modified-DC vaccine in patients with resected NSCLC
    Authors: C Ge, R Li, H Song, T Geng, J Yang, Q Tan, L Song, Y Wang, Y Xue, Z Li, S Dong, Z Zhang, N Zhang, J Guo, L Hua, S Chen, X Song
    BMC Cancer, 2017-12-21;17(1):884.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. The Immunogenicity of Branded and Biosimilar Infliximab in Rheumatoid Arthritis According to Th9-Related Responses
    Authors: R Talotta, A Berzi, A Doria, A Batticciot, MC Ditto, F Atzeni, P Sarzi-Putt, D Trabattoni
    Int J Mol Sci, 2017-10-12;18(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Impact of Vaccination on Distribution of T Cell Subsets in Antiretroviral-Treated HIV-Infected Children
    Authors: P Thitilertd, L Khowawiset, P Ammaranond, P Poungpairo, V Tantithavo, N Onlamoon
    Dis. Markers, 2017-06-12;2017(0):5729639.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. E. coli promotes human V?9V?2?T cell transition from cytokine-producing bactericidal effectors to professional phagocytic killers in a TCR-dependent manner
    Authors: M Barisa, AM Kramer, Y Majani, D Moulding, L Saraiva, M Bajaj-Elli, J Anderson, K Gustafsson
    Sci Rep, 2017-06-05;7(1):2805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Autosomal dominant gain of function STAT1 mutation and severe bronchiectasis
    Authors: O Breuer, H Daum, M Cohen-Cymb, S Unger, D Shoseyov, P Stepensky, B Keller, K Warnatz, E Kerem
    Respir Med, 2017-03-22;126(0):39-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Role of Occult and Post-acute Phase Replication in Protective Immunity Induced with a Novel Live Attenuated SIV Vaccine
    PLoS Pathog, 2016-12-21;12(12):e1006083.
    Species: Primate
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Humoral and cellular response after varicella vaccination in VZV IgG seronegative kidney transplant candidates
    Authors: Marcia M L Kho
    Vaccine, 2016-11-26;35(1):71-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Cognate HLA absence in trans diminishes human NK cell education
    J Clin Invest, 2016-08-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
    Authors: Kellie N Smith
    MBio, 2016-05-31;7(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Different maturation cocktails provide dendritic cells with different chemoattractive properties.
    Authors: Massa C, Thomas C, Wang E, Marincola F, Seliger B
    J Transl Med, 2015-06-03;13(1):175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Characterization of CD8+ T cell differentiation following SIVDeltanef vaccination by transcription factor expression profiling.
    Authors: Billingsley J, Rajakumar P, Connole M, Salisch N, Adnan S, Kuzmichev Y, Hong H, Reeves R, Kang H, Li W, Li Q, Haase A, Johnson R
    PLoS Pathog, 2015-03-13;11(3):e1004740.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.
    Authors: Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer B, Kuppers R
    Proc Natl Acad Sci U S A, 2015-01-26;112(6):E546-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
    Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
    Proc Natl Acad Sci U S A, 2015-01-23;112(6):E556-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Human regulatory T cells lack the cyclophosphamide-extruding transporter ABCB1 and are more susceptible to cyclophosphamide-induced apoptosis.
    Authors: Dimeloe S, Frick C, Fischer M, Gubser P, Razik L, Bantug G, Ravon M, Langenkamp A, Hess C
    Eur J Immunol, 2014-10-27;44(12):3614-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Treatment with chemotherapy and dendritic cells pulsed with multiple Wilms' tumor 1 (WT1)-specific MHC class I/II-restricted epitopes for pancreatic cancer.
    Authors: Koido S, Homma S, Okamoto M, Takakura K, Mori M, Yoshizaki S, Tsukinaga S, Odahara S, Koyama S, Imazu H, Uchiyama K, Kajihara M, Arakawa H, Misawa T, Toyama Y, Yanagisawa S, Ikegami M, Kan S, Hayashi K, Komita H, Kamata Y, Ito M, Ishidao T, Yusa S, Shimodaira S, Gong J, Sugiyama H, Ohkusa T, Tajiri H
    Clin Cancer Res, 2014-07-23;20(16):4228-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. A thymic stromal lymphopoietin-responsive dendritic cell subset mediates allergic responses in the upper airway mucosa.
    Authors: Melum G, Farkas L, Scheel C, Van Dieren B, Gran E, Liu Y, Johansen F, Jahnsen F, Baekkevold E
    J Allergy Clin Immunol, 2014-06-21;134(3):613-621.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Dasatinib promotes Th1-type responses in granzyme B expressing T-cells.
    Authors: Kreutzman A, Ilander M, Porkka K, Vakkila J, Mustjoki S
    Oncoimmunology, 2014-05-29;3(0):e28925.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Chemokines as novel and versatile reagents for flow cytometry and cell sorting.
    Authors: Le Brocq M, Fraser A, Cotton G, Woznica K, McCulloch C, Hewit K, McKimmie C, Nibbs R, Campbell J, Graham G
    J Immunol, 2014-05-21;192(12):6120-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. IL-2 phosphorylates STAT5 to drive IFN-gamma production and activation of human dendritic cells.
    Authors: Herr F, Lemoine R, Gouilleux F, Meley D, Kazma I, Heraud A, Velge-Roussel F, Baron C, Lebranchu Y
    J Immunol, 2014-05-14;192(12):5660-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells.
    Authors: Wolfl M, Greenberg P
    Nat Protoc, 2014-03-27;9(4):950-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Manipulating the expression of chemokine receptors enhances delivery and activity of cytokine-induced killer cells.
    Authors: Zou Y, Li F, Hou W, Sampath P, Zhang Y, Thorne S
    Br J Cancer, 2014-03-18;110(8):1992-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. Enlarged memory T-cell pool and enhanced Th1-type responses in chronic myeloid leukemia patients who have successfully discontinued IFN-alpha monotherapy.
    Authors: Ilander M, Kreutzman A, Rohon P, Melo T, Faber E, Porkka K, Vakkila J, Mustjoki S
    PLoS ONE, 2014-01-31;9(1):e87794.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. The road to memory: an early rest for the long journey.
    Authors: Ho, Liam Poc, Yit, Pui San, Ng, Lee Hui, Linn, Yeh Chin, Zhao, Yi, Sun, Li, Ling, Khoon Li, Chai Koh, Mickey B, Monica Shih, Meng-Chu, Li, Shang, Wang, Xue Ying, Tien, Sim Leng, Goh, Yeow Tee
    J Immunol, 2013-11-01;191(11):5603-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  43. Conversion of peripheral blood NK cells to a decidual NK-like phenotype by a cocktail of defined factors.
    Authors: Cerdeira A, Rajakumar A, Royle C, Lo A, Husain Z, Thadhani R, Sukhatme V, Karumanchi S, Kopcow H
    J Immunol, 2013-03-13;190(8):3939-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  44. Pertussis circulation has increased T-cell immunity during childhood more than a second acellular booster vaccination in Dutch children 9 years of age.
    Authors: Schure R, de Rond L, Ozturk K, Hendrikx L, Sanders E, Berbers G, Buisman A
    PLoS ONE, 2012-07-31;7(7):e41928.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  45. Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
    Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
    J Transl Med, 2012-07-12;10(0):146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  46. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011-09-01;6(9):e23741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  47. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  48. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2009-12-11;184(2):1102-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.
    Authors: Damas JK, Landro L, Fevang B, Heggelund L, Tjonnfjord GE, Floisand Y, Halvorsen B, Froland SS, Aukrust P
    Clin. Exp. Immunol., 2009-09-01;157(3):400-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  50. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
    Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2009-07-05;10(8):857-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  51. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  52. Dissociation of its opposing immunologic effects is critical for the optimization of antitumor CD8+ T-cell responses induced by interleukin 21.
    Authors: Ansen S, Butler MO, Berezovskaya A, Murray AP, Stevenson K, Nadler LM, Hirano N
    Clin. Cancer Res., 2008-10-01;14(19):6125-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  53. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008-07-14;205(8):1797-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  54. IL-12-impaired and IL-12-secreting dendritic cells produce IL-23 upon CD154 restimulation.
    Authors: Jasny E, Eisenblatter M, Matz-Rensing K, Tenner-Racz K, Tenbusch M, Schrod A, Stahl-Hennig C, Moos V, Schneider T, Racz P, Uberla K, Kaup FJ, Ignatius R
    J. Immunol., 2008-05-15;180(10):6629-39.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  55. Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
    Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
    FASEB J., 2008-03-25;22(8):2747-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  56. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
    Cancer Res., 2008-02-01;68(3):790-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  57. Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions.
    Authors: Pokkali S, Das SD, R L
    Cytokine, 2008-01-28;41(3):307-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  58. BAFF enhances chemotaxis of primary human B cells: a particular synergy between BAFF and CXCL13 on memory B cells.
    Authors: Badr G, Borhis G, Lefevre EA, Chaoul N, Deshayes F, Dessirier V, Lapree G, Tsapis A, Richard Y
    Blood, 2008-01-02;111(5):2744-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  59. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007-08-05;8(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  60. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2006-12-29;109(9):3649-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  61. Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus.
    Authors: Kaur A, Sanford HB, Garry D, Lang S, Klumpp SA, Watanabe D, Bronson RT, Lifson JD, Rosati M, Pavlakis GN, Felber BK, Knipe DM, Desrosiers RC
    Virology, 2006-09-07;357(2):199-214.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  62. Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10.
    Authors: Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH
    J. Immunol., 2006-07-01;177(1):593-603.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  63. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006-05-01;118(9):2147-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  64. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006-04-13;108(3):947-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  65. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004-07-19;101(30):11064-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  66. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-03-18;104(2):453-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  67. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003-09-01;112(3):556-62.
    Species: Human
    Sample Types: BALF
    Applications: Flow Cytometry
  68. Human cerebrospinal fluid central memory CD4+ T cells: evidence for trafficking through choroid plexus and meninges via P-selectin.
    Authors: Kivisakk P, Mahad DJ, Callahan MK, Trebst C, Tucky B, Wei T, Wu L, Baekkevold ES, Lassmann H, Staugaitis SM, Campbell JJ, Ransohoff RM
    Proc. Natl. Acad. Sci. U.S.A., 2003-06-26;100(14):8389-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  69. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma
    Authors: Yukari Kobayashi, Daisuke Yamada, Taketo Kawai, Yusuke Sato, Taro Teshima, Yuta Yamada et al.
    International Journal of Oncology
  70. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2
    Authors: Jil Sander, Susanne V. Schmidt, Branko Cirovic, Naomi McGovern, Olympia Papantonopoulou, Anna-Lena Hardt et al.
    Immunity
  71. Sustained protection against Ebola virus infection following treatment of infected nonhuman primates with ZMAb
    Authors: Xiangguo Qiu, Jonathan Audet, Gary Wong, Lisa Fernando, Alexander Bello, Stéphane Pillet et al.
    Scientific Reports

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Human CCR7 PE-conjugated Antibody
By Anonymous on 06/04/2021
Application: Flow Sample Tested: Preipheral blood monocytes Species: Human
Flow Cytometry Human CCR7 PE-conjugated Antibody FAB197P
Human CCR7 PE-conjugated Antibody
By Anonymous on 07/14/2016
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

PBMC stained with anti -CD3 and CCR7-PE at various concentrations as mentioned in the figure. Cells were then fixed (BD fix) and ran on a BD Fortessa.

This clone works for staining PBMC but not whole blood.
When staining whole blood, the PE version gives a non-specific staining in all leukocyte populations. The FITC version stains lymphocytes preferentially but with a small shift.

Flow Cytometry Human CCR7 PE-conjugated Antibody FAB197P
Human CCR7 PE‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

Flow Cytometry Human CCR7 PE‑conjugated Antibody FAB197P