Human CCR5 Antibody

Catalog # Availability Size / Price Qty
MAB181-100
MAB181-SP
CCR5 in Human Dorsal Root Ganglia.
6 Images
Product Details
Citations (17)
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Human CCR5 Antibody Summary

Species Reactivity
Human
Specificity
Can be used to detect CCR5 present on stimulated human PBMCs only after cells are fixed with 2% formaldehyde. It does not detect CCR5 on unfixed human PBMCs. It does not cross-react with CCR1, CCR2, or CCR3. For additional information regarding epitope specificity for this antibody and other R&D Systems anti-human CCR5 antibodies, see Lee, B. et al., 1999, J. Biol. Chem. 274:9617.
Source
Monoclonal Mouse IgG2B Clone # 45523
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CCR5 antibody in Human Dorsal Root Ganglia by Immunohistochemistry (IHC-P). View Larger

CCR5 in Human Dorsal Root Ganglia. CCR5 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 25 µg/mL Human CCR5 Monoclonal Antibody (Catalog # MAB181) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Detection of Human CCR5 by Immunohistochemistry View Larger

Detection of Human CCR5 by Immunohistochemistry Presence of potential HIV target cells in the human prostate. Immunohistochemistry on uninfected prostate sections before culture showed the presence of periglandular foci of HLA-DR+ (A) and CD4+ cells (B, serial section with A) as well as scattered stromal cells staining positive for HLA-DR (A), CD4 (B), CD3 (C), CD68 (D), CCR5 (E) and CXCR4 (F). The arrows point out immune cells inserted within the epithelium-Scale bars = 50 μm; (G): the respective proportions of CD3, CD4, CD68, CXCR4 and CCR5+ cells per surface unit were evaluated on whole prostate sections from a minimum of 3 donors whose explants were subsequently exposed to HIV-1 strains. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/19117522), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human CCR5 by Western Blot View Larger

Detection of Human CCR5 by Western Blot TGF-beta signaling regulated the expression of CCR5(A) 3×105 MDA-MB-231 and MCF-7 cells were stimulated with 1-5ng/ml TGF-beta 1 for 24 h, and total RNA was isolated and tested for CCR5 mRNA by quantitative PCR. (B) Western blot for CCR5 protein in breast cancer cells (106) under TGF-beta 1 stimulation for 48 h. Data presented were representatives of at least three independent experiments. (C) MDA-MB-231 and MCF-7 cells (3×105) were co-transfected with pGL3-CCR5 and pRL-TK and exposed to different concentrations of TGF-beta 1 for 24 h, and luciferase activities were determined. (D) MDA-MB-231 and MCF-7 cells were pre-treated with 5μM SIS3 for 2 h, and cells were subjected to luciferase assay. (E) 106 MCF-7 cells were transfected with TGF beta RI/ALK5 siRNA, and were then co-cultured with lactate-activated THP-1 macrophages (ratio 1:1) for 24 h. The protein levels of CCR5 were assayed by western blot. (F) The expression of TGF-beta 1, CCL5 and CCR5 in clinical samples obtained from breast cancer patients. The mRNA levels were measured by quantitative PCR, and the correlation between TGF-beta 1 and CCL5-CCR5 axis was shown. (G) Representative IHC staining for TGF-beta 1, CCL5 and CCR5 in breast cancer samples. The sample used was derived from 28 breast cancer cases. Scale bars represent 50 μm. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CCR5 by Immunohistochemistry View Larger

Detection of Human CCR5 by Immunohistochemistry Macrophages promoted breast cancer metastasis through CCL5(A) MDA-MB-231 cells were co-cultured with 15 mM lactate-activated THP-1 macrophages for 7 days, in the presence of 5μg/ml anti-CCL5 neutralizing antibody or not. MDA-MB-231 cells were then collected and injected into the tail vein of nude mice. After two weeks, animals were sacrificed and metastatic nodules on lung surfaces were counted. (B) CCR5, HK2 and p-AMPK were immunostained in MDA-MB-231 metastases. Scale bars represent 50 μm. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human CCR5 by Western Blot View Larger

Detection of Human CCR5 by Western Blot Lactate-activated macrophages induced EMT in breast cancer cells through CCL5-CCR5 axis(A) 106 THP-1 macrophages were treated with 15 mM lactate for 72 h, and then cells were washed twice and fresh media were added. Macrophages were cultured for another 24 h and the conditional media (lactate CM) was collected. The effect of CM on breast cancer cell migration was measured by double chamber transwell assay. 5μg/ml anti-CCL5 neutralizing antibody significantly decreased lactate CM-induced cell migration. (B) 106 MCF-7 cells were co-cultured with 15 mM lactate-activated macrophages in the presence of 5μg/ml anti-CCL5 antibody or not, and protein levels of EMT markers were tested by western blot. (C) 106 breast cancer cells were co-cultured with 106 lactate-activated THP-1 macrophages (or 106 lactate-activated primary macrophages) for different time points, and the expression of CCR5 was monitored by western blot. (D) MDA-MB-231 and MCF-7 cells were transfected with shCCR5 plasmids, or pre-treated with 5μM Maraviroc for 2 h, then cell migration induced by lactate CM was detected by double chamber transwell assay. Lactate CM was described in (A). (E) MCF-7 cells (106) were transfected with pcDNA3.1-CCR5, and then cultured with 10ng/ml CCL5 for 24 h. The expression of E-cadherin, N-cadherin and vimentin was investigated by western blot. (F) 106 Human primary macrophages (No. 4 and No. 9) were treated with 15 mM lactate for 72 h and CM was collected as described in (A). The migration of MDA-MB-231 cells was measured in the presence of primary macrophage CM. 5μg/ml anti-CCL5 neutralizing antibody, shRNAs designed against CCR5, or 5μM Maraviroc, significantly reduced primary macrophage CM-induced cell migration. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CCR5 by Immunohistochemistry View Larger

Detection of Human CCR5 by Immunohistochemistry CCL5-CCR5 axis induced aerobic glycolysis by regulation of AMPK signaling(A) Western blot for AMPK, c-Myc, HIF-1 alpha and Akt in breast cancer cells co-cultured with 15mM lactic acid-activated THP-1 macrophages (ratio 1:1) for 72 h. Results presented were representatives of at least three independent experiments. (B) The expression of AMPK downstream signaling target ACC in breast cancer cells co-cultured as in (A). (C) MDA-MB-231 and MCF-7 cells were transfected with 50 nM AMPK alpha 1 siRNA, or pretreated with 10μM compound C for 4 h, and then incubated with 15mM lactic acid-activated THP-1 macrophages (ratio 1:1) for 48 h. The glucose uptake, lactic acid production and ATP levels were detected. (D) The inhibition of AMPK abrogated macrophage-induced EMT in MCF-7 cells. Cells were treated as described in (C). After co-culture, the expression of EMT markers, E-cadherin and vimentin, was measured by western blot. (E) Recombinant human CCL5 induced the phosphorylation of AMPK in MDA-MB-231 and MCF-7/CCR5 cells. 106 cells were treated with 50ng/ml CCL5 for defferent time points as indicated, and phosphorylated AMPK and total AMPK were investigated by western blot. (F) Inhibition of CCR5 in MDA-MB-231 cells significantly attenuated macrophage-induced AMPK phosphorylation. MDA-MB-231 cells were transfected with shRNAs designed against CCR5, or pre-treated with 5μM Maraviroc for 2 h, then co-cultured with 15 mM lactate-activated macrophages as described in (A). After co-culture, the phosphorylation of AMPK was detected by western blot. (G) Expressions of CCL5, CCR5 and p-AMPK in samples obtained from breast cancer patients (n =28). Scale bars represent 50 μm. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

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Citations for Human CCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells
    Authors: James M. Fox, Richard Kasprowicz, Oliver Hartley, Nathalie Signoret
    Journal of Leukocyte Biology
  2. CXCR4 Recognition by L- and D-Peptides Containing the Full-Length V3 Loop of HIV-1 gp120
    Authors: Zhu, R;Sang, X;Zhou, J;Meng, Q;Huang, LSM;Xu, Y;An, J;Huang, Z;
    Viruses
    Species: Chinese Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies
    Authors: M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic
    Journal of Translational Medicine, 2022-01-24;20(1):39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5
    Authors: G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai
    Cell Death Dis, 2019-03-19;10(4):264.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Primary peripheral T-cell lymphoma, not otherwise specified of the thyroid with autoimmune thyroiditis.
    Authors: Yoshida N, Nishikori M, Izumi T, Imaizumi Y, Sawayama Y, Niino D, Tashima M, Hoshi S, Ohshima K, Shimoyama M, Seto M, Tsukasaki K
    Br J Haematol, 2013-02-21;161(2):214-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
    Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
    Nature, 2012-12-12;493(7430):51-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008-07-01;8(7):1512-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006-12-01;169(6):2094-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.
    Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
    AIDS Res. Hum. Retroviruses, 2006-11-01;22(11):1152-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis.
    Authors: Capelli A, Di Stefano A, Gnemmi I, Donner CF
    Eur. Respir. J., 2005-04-01;25(4):701-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
    Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
    Cell. Signal., 2005-03-01;17(3):355-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004-12-01;34(12):3485-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Chemokines and chemokine receptors in inflammatory demyelinating neuropathies: a central role for IP-10.
    Authors: Kieseier BC, #N*´)2ZP, Tani M, y§/Ôc؝Þ, Mahad D, Oka N, Ho T, Woodroofe N, Griffin JW, Toyka KV, Ransohoff RM, Hartung HP
    2002-04-01;125(0):823-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
    Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
    J. Biol. Chem., 1999-04-02;274(14):9617-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop
    Authors: Sensen Lin, Li Sun, Xiaodan Lyu, Xiongfei Ai, Danyu Du, Nan Su et al.
    Oncotarget
  16. A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
    Authors: Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe
    Communications Biology
  17. Vitamin D Status Impacts Genital Mucosal Immunity and Markers of HIV-1 Susceptibility in Women
    Authors: Sharon M. Anderson, Andrea R. Thurman, Neelima Chandra, Suzanne S. Jackson, Susana Asin, Christiane Rollenhagen et al.
    Nutrients

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