Human CCR5 Antibody Summary
Met1-Leu352
Accession # P51681
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
CCR5 in Human Dorsal Root Ganglia. CCR5 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 25 µg/mL Human CCR5 Monoclonal Antibody (Catalog # MAB181) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human CCR5 by Immunohistochemistry Presence of potential HIV target cells in the human prostate. Immunohistochemistry on uninfected prostate sections before culture showed the presence of periglandular foci of HLA-DR+ (A) and CD4+ cells (B, serial section with A) as well as scattered stromal cells staining positive for HLA-DR (A), CD4 (B), CD3 (C), CD68 (D), CCR5 (E) and CXCR4 (F). The arrows point out immune cells inserted within the epithelium-Scale bars = 50 μm; (G): the respective proportions of CD3, CD4, CD68, CXCR4 and CCR5+ cells per surface unit were evaluated on whole prostate sections from a minimum of 3 donors whose explants were subsequently exposed to HIV-1 strains. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/19117522), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCR5 by Western Blot TGF-beta signaling regulated the expression of CCR5(A) 3×105 MDA-MB-231 and MCF-7 cells were stimulated with 1-5ng/ml TGF-beta 1 for 24 h, and total RNA was isolated and tested for CCR5 mRNA by quantitative PCR. (B) Western blot for CCR5 protein in breast cancer cells (106) under TGF-beta 1 stimulation for 48 h. Data presented were representatives of at least three independent experiments. (C) MDA-MB-231 and MCF-7 cells (3×105) were co-transfected with pGL3-CCR5 and pRL-TK and exposed to different concentrations of TGF-beta 1 for 24 h, and luciferase activities were determined. (D) MDA-MB-231 and MCF-7 cells were pre-treated with 5μM SIS3 for 2 h, and cells were subjected to luciferase assay. (E) 106 MCF-7 cells were transfected with TGF beta RI/ALK5 siRNA, and were then co-cultured with lactate-activated THP-1 macrophages (ratio 1:1) for 24 h. The protein levels of CCR5 were assayed by western blot. (F) The expression of TGF-beta 1, CCL5 and CCR5 in clinical samples obtained from breast cancer patients. The mRNA levels were measured by quantitative PCR, and the correlation between TGF-beta 1 and CCL5-CCR5 axis was shown. (G) Representative IHC staining for TGF-beta 1, CCL5 and CCR5 in breast cancer samples. The sample used was derived from 28 breast cancer cases. Scale bars represent 50 μm. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCR5 by Immunohistochemistry Macrophages promoted breast cancer metastasis through CCL5(A) MDA-MB-231 cells were co-cultured with 15 mM lactate-activated THP-1 macrophages for 7 days, in the presence of 5μg/ml anti-CCL5 neutralizing antibody or not. MDA-MB-231 cells were then collected and injected into the tail vein of nude mice. After two weeks, animals were sacrificed and metastatic nodules on lung surfaces were counted. (B) CCR5, HK2 and p-AMPK were immunostained in MDA-MB-231 metastases. Scale bars represent 50 μm. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCR5 by Western Blot Lactate-activated macrophages induced EMT in breast cancer cells through CCL5-CCR5 axis(A) 106 THP-1 macrophages were treated with 15 mM lactate for 72 h, and then cells were washed twice and fresh media were added. Macrophages were cultured for another 24 h and the conditional media (lactate CM) was collected. The effect of CM on breast cancer cell migration was measured by double chamber transwell assay. 5μg/ml anti-CCL5 neutralizing antibody significantly decreased lactate CM-induced cell migration. (B) 106 MCF-7 cells were co-cultured with 15 mM lactate-activated macrophages in the presence of 5μg/ml anti-CCL5 antibody or not, and protein levels of EMT markers were tested by western blot. (C) 106 breast cancer cells were co-cultured with 106 lactate-activated THP-1 macrophages (or 106 lactate-activated primary macrophages) for different time points, and the expression of CCR5 was monitored by western blot. (D) MDA-MB-231 and MCF-7 cells were transfected with shCCR5 plasmids, or pre-treated with 5μM Maraviroc for 2 h, then cell migration induced by lactate CM was detected by double chamber transwell assay. Lactate CM was described in (A). (E) MCF-7 cells (106) were transfected with pcDNA3.1-CCR5, and then cultured with 10ng/ml CCL5 for 24 h. The expression of E-cadherin, N-cadherin and vimentin was investigated by western blot. (F) 106 Human primary macrophages (No. 4 and No. 9) were treated with 15 mM lactate for 72 h and CM was collected as described in (A). The migration of MDA-MB-231 cells was measured in the presence of primary macrophage CM. 5μg/ml anti-CCL5 neutralizing antibody, shRNAs designed against CCR5, or 5μM Maraviroc, significantly reduced primary macrophage CM-induced cell migration. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCR5 by Immunohistochemistry CCL5-CCR5 axis induced aerobic glycolysis by regulation of AMPK signaling(A) Western blot for AMPK, c-Myc, HIF-1 alpha and Akt in breast cancer cells co-cultured with 15mM lactic acid-activated THP-1 macrophages (ratio 1:1) for 72 h. Results presented were representatives of at least three independent experiments. (B) The expression of AMPK downstream signaling target ACC in breast cancer cells co-cultured as in (A). (C) MDA-MB-231 and MCF-7 cells were transfected with 50 nM AMPK alpha 1 siRNA, or pretreated with 10μM compound C for 4 h, and then incubated with 15mM lactic acid-activated THP-1 macrophages (ratio 1:1) for 48 h. The glucose uptake, lactic acid production and ATP levels were detected. (D) The inhibition of AMPK abrogated macrophage-induced EMT in MCF-7 cells. Cells were treated as described in (C). After co-culture, the expression of EMT markers, E-cadherin and vimentin, was measured by western blot. (E) Recombinant human CCL5 induced the phosphorylation of AMPK in MDA-MB-231 and MCF-7/CCR5 cells. 106 cells were treated with 50ng/ml CCL5 for defferent time points as indicated, and phosphorylated AMPK and total AMPK were investigated by western blot. (F) Inhibition of CCR5 in MDA-MB-231 cells significantly attenuated macrophage-induced AMPK phosphorylation. MDA-MB-231 cells were transfected with shRNAs designed against CCR5, or pre-treated with 5μM Maraviroc for 2 h, then co-cultured with 15 mM lactate-activated macrophages as described in (A). After co-culture, the phosphorylation of AMPK was detected by western blot. (G) Expressions of CCL5, CCR5 and p-AMPK in samples obtained from breast cancer patients (n =28). Scale bars represent 50 μm. *, P<0.05; **, P<0.01. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22786), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR5
CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.
Product Datasheets
Citations for Human CCR5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells
Authors: James M. Fox, Richard Kasprowicz, Oliver Hartley, Nathalie Signoret
Journal of Leukocyte Biology
-
CXCR4 Recognition by L- and D-Peptides Containing the Full-Length V3 Loop of HIV-1 gp120
Authors: Zhu, R;Sang, X;Zhou, J;Meng, Q;Huang, LSM;Xu, Y;An, J;Huang, Z;
Viruses
Species: Chinese Hamster
Sample Types: Whole Cells
Applications: Bioassay -
Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies
Authors: M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic
Journal of Translational Medicine, 2022-01-24;20(1):39.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5
Authors: G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai
Cell Death Dis, 2019-03-19;10(4):264.
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC-P -
Primary peripheral T-cell lymphoma, not otherwise specified of the thyroid with autoimmune thyroiditis.
Authors: Yoshida N, Nishikori M, Izumi T, Imaizumi Y, Sawayama Y, Niino D, Tashima M, Hoshi S, Ohshima K, Shimoyama M, Seto M, Tsukasaki K
Br J Haematol, 2013-02-21;161(2):214-23.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
Nature, 2012-12-12;493(7430):51-5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
Am. J. Transplant., 2008-07-01;8(7):1512-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
Am. J. Pathol., 2006-12-01;169(6):2094-103.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.
Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
AIDS Res. Hum. Retroviruses, 2006-11-01;22(11):1152-61.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis.
Authors: Capelli A, Di Stefano A, Gnemmi I, Donner CF
Eur. Respir. J., 2005-04-01;25(4):701-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
Cell. Signal., 2005-03-01;17(3):355-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
Eur. J. Immunol., 2004-12-01;34(12):3485-96.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemokines and chemokine receptors in inflammatory demyelinating neuropathies: a central role for IP-10.
Authors: Kieseier BC, #N*´)2ZP, Tani M, y§/ÔcØÞ, Mahad D, Oka N, Ho T, Woodroofe N, Griffin JW, Toyka KV, Ransohoff RM, Hartung HP
2002-04-01;125(0):823-34.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
J. Biol. Chem., 1999-04-02;274(14):9617-26.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop
Authors: Sensen Lin, Li Sun, Xiaodan Lyu, Xiongfei Ai, Danyu Du, Nan Su et al.
Oncotarget
-
A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines
Authors: Shengli Song, Miriam Manook, Jean Kwun, Annette M. Jackson, Stuart J. Knechtle, Garnett Kelsoe
Communications Biology
-
Vitamin D Status Impacts Genital Mucosal Immunity and Markers of HIV-1 Susceptibility in Women
Authors: Sharon M. Anderson, Andrea R. Thurman, Neelima Chandra, Suzanne S. Jackson, Susana Asin, Christiane Rollenhagen et al.
Nutrients
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human CCR5 Antibody
There are currently no reviews for this product. Be the first to review Human CCR5 Antibody and earn rewards!
Have you used Human CCR5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image