Home / CCR3 / Human CCR3 Fluorescein-conjugated Antibody

Human CCR3 Fluorescein-conjugated Antibody

Catalog #: FAB155F
10 Citations Datasheet / COA / SDS
Clone 61828 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB155F-025
FAB155F-100
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry.
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Product Details
Citations (10)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Source
Monoclonal Rat IgG2A Clone # 61828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Integrin a4/CD49d APC-conjugated Monoclonal Antibody (Catalog # FAB1354A) and either (A) Rat Anti-Human CCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB155F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Human CCR3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
    Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
    iScience, 2021-11-14;24(12):103433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity
    Authors: Lee Faulkner
    Toxicol. Sci, 2016-09-16;154(2):416-429.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007-07-19;171(3):767-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2007-03-09;36(5):237-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007-02-01;62(2):170-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Anti-IL-5 (mepolizumab) therapy for eosinophilic esophagitis.
    Authors: Stein ML, Collins MH, Villanueva JM, Kushner JP, Putnam PE, Buckmeier BK, Filipovich AH, Assa'ad AH, Rothenberg ME
    J. Allergy Clin. Immunol., 2006-11-07;118(6):1312-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle.
    Authors: Ameredes BT, Calhoun WJ
    J. Allergy Clin. Immunol., 2005-07-01;116(1):65-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis.
    Authors: Wang CR, Liu MF
    Clin. Exp. Immunol., 2003-05-01;132(2):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003-05-01;73(5):584-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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