Human CCR3 Antibody
Human CCR3 Antibody Summary
Accession # P51677
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![CCR3 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). CCR3 antibody in Human Lymph Node by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/CCR3_MAB155_Immunohistochemistry_6950.jpg)
CCR3 in Human Lymph Node. CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
![Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody. Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody.](https://resources.rndsystems.com/images/datasheets/antibody/CCR3_MAB155_Block_Neutralize_9028.jpg)
Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody. Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND50 is typically 0.05-0.25 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR3
CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.
Product Datasheets
Citations for Human CCR3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines
Authors: Y Kaibori, D Nagakubo
Cancer Gene Therapy, 2022-09-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells
Authors: MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow
MBio, 2021-08-17;12(4):e0196221.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage
Authors: Michal Segal-Salto, Neta Barashi, Avi Katav, Vicktoria Edelshtein, Arnon Aharon, Sharon Hashmueli et al.
JHEP Reports
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Evidence for chemokine synergy during neutrophil migration in ARDS
Thorax, 2016-08-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells
Authors: Guichun Huang
Sci Rep, 2016-06-02;6(0):27152.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression and prognostic significance of CCL11/CCR3 in glioblastoma
Oncotarget, 2016-05-31;7(22):32617-27.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature, 2011-07-13;475(7355):226-30.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
Int. J. Cancer, 2010-07-15;127(2):332-44.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
J. Immunol., 2008-12-01;181(11):8053-67.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC-P, Neutralization -
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
Stem Cells, 2007-03-29;25(7):1737-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells.
Authors: Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD
Eur. J. Immunol., 2006-10-01;36(10):2700-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
J. Immunol., 2006-09-01;177(5):3344-54.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion.
Authors: Spencer LA, Melo RC, Perez SA, Bafford SP, Dvorak AM, Weller PF
Proc. Natl. Acad. Sci. U.S.A., 2006-02-21;103(9):3333-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N
J. Allergy Clin. Immunol., 2005-12-02;117(1):103-10.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
J. Immunol., 2004-12-01;173(11):6592-602.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood, 2003-12-04;103(7):2806-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
J. Leukoc. Biol., 2003-05-01;73(5):584-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
Authors: Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ
J. Exp. Med., 2002-09-16;196(6):781-91.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
Eur. J. Immunol., 2001-04-01;31(4):1037-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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