Human CCR11 Antibody

Catalog #: MAB6899 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6899
MAB6899-SP

Detection of CCR11 in HEK293 Human Cell Line Transfected with Human CCR11 and eGFP by Flow Cytometry.

1 Image

All CCR11 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR11 in direct ELISAs.

Source

Monoclonal Mouse IgM Clone # 674144

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CCR11
Accession # Q9NPB9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CCR11 in HEK293 Human Cell Line Transfected with Human CCR11 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human CCR11 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CCR11 Monoclonal Antibody (Catalog # MAB6899) followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR11

CCR11, also known as CCRL1 and CCX-CKR, is a 45 kDa 7-transmembrane segment protein that is widely expressed on stromal cells. It is a silent receptor that does not induce intracellular calcium signaling, but instead serves as a chemokine scavenger receptor. CCR11 regulates dendritic cell and thymic precursor migration by mediating the clearance of the homeostatic chemokines CCL19, 21, and 25. It is upregulated on some breast cancer cells and on bronchial epithelial cells in pulmonary sarcoidosis. Human CCR11 shares 85% amino acid sequence identity with mouse and rat CCR11.

Entrez Gene IDs

51554 (Human); 252837 (Mouse); 685243 (Rat)

Alternate Names

ACKR4; CC chemokine receptor-like 1; C-C CKR-11; CCBP2CCX CKR; CC-CKR-11; CCR11; CCR11CCR10; CCRL1; CCX-CKR; chemokine (C-C motif) receptor-like 1; orphan seven-transmembrane receptor, chemokine related; PPR1; PPR1cc motif, receptor-like protein 1; VSHK1; VSHK1CKR-11