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Human CCL3/MIP-1 alpha DuoSet ELISA

Catalog #: DY270
54 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY270
DY270-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CCL3 / MIP-1 alpha ELISA Standard Curve
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Product Details
Procedure
Citations (54)
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Supplemental Products
Reviews (4)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CCL3/MIP-1 alpha DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL3/MIP-1alpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CCL3 / MIP-1 alpha ELISA Standard Curve View Larger

Human CCL3 / MIP-1 alpha ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL3/MIP-1 alpha

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

Entrez Gene IDs:
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names:
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human CCL3/MIP-1 alpha DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

54 Citations: Showing 1 - 10
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  1. Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls
    Authors: Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M;
    PloS one
    Species: Human
    Sample Types: Vaginal Swab
  2. Empowering Naringin's Anti-Inflammatory Effects through Nanoencapsulation
    Authors: Marinho, A;Seabra, CL;Lima, SAC;Lobo-da-Cunha, A;Reis, S;Nunes, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses
    Authors: Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS;
    Biomedicines
    Species: Human
    Sample Types: Whole Blood
  4. Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis
    Authors: Kim, YT;Huh, JW;Choi, YH;Yoon, HK;Nguyen, TT;Chun, E;Jeong, G;Park, S;Ahn, S;Lee, WK;Noh, YW;Lee, KS;Ahn, HS;Lee, C;Lee, SM;Kim, KS;Suh, GJ;Jeon, K;Kim, S;Jin, M;
    EMBO molecular medicine
    Species: Mouse
    Sample Types: Plasma, Peritoneal Lavage Fluid
  5. Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial
    Authors: Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D;
    PloS one
    Species: Human
    Sample Types: Serum
  6. ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis
    Authors: Y Xie, A Chupina Es, B Nelson, H Feng, C Pothoulaki, L Chesnel, HW Koon
    Frontiers in Microbiology, 2023-01-10;13(0):1072534.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Tryptophan-dependent and -independent secretions of tryptophanyl- tRNA synthetase mediate innate inflammatory responses
    Authors: TTT Nguyen, YH Choi, WK Lee, Y Ji, E Chun, YH Kim, JE Lee, HS Jung, JH Suh, S Kim, M Jin
    Cell Reports, 2022-12-30;42(1):111905.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Exercise training-induced changes in immunometabolic markers in youth badminton athletes
    Authors: FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi
    Scientific Reports, 2022-09-15;12(1):15539.
    Species: Human
    Sample Types: Plasma
  9. Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response
    Authors: MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges
    Journal of Immunology Research, 2022-02-25;2022(0):9926305.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Inflammatory responses to metal oxide ceramic nanopowders
    Authors: S Jamieson, A Mawdesley, D Deehan, J Kirby, J Holland, A Tyson-Capp
    Scientific Reports, 2021-05-18;11(1):10531.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Systems view of Bordetella pertussis booster vaccination in adults primed with whole-cell vs. acellular vaccine
    Authors: R da Silva A, F Soldevila, M Pomaznoy, M Babor, J Bennett, Y Tian, NN Khalil, Y Qian, A Mandava, RH Scheuerman, M Cortese, B Pulendran, CD Petro, AP Gilkes, LA Purcell, A Sette, B Peters
    JCI Insight, 2021-04-08;0(0):.
    Species: Human
    Sample Types: Plasma
  12. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  13. Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
    Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
    Frontiers in Pharmacology, 2020-12-08;11(0):598702.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. High circulating SDF-1and MCP-1 levels and genetic variations in CXCL12, CCL2 and CCR5: Prognostic signature of immune recovery status in treated HIV-positive patients
    Authors: E Yeregui, C Viladés, P Domingo, A Ceausu, YM Pacheco, S Veloso, A Inciarte, J Vidal-Gonz, M Peraire, C Perpiñán, V Falcó, J Masip, V Alba, M Vargas, A Martí, L Reverté, J Mallolas, F Vidal, J Peraire, A Rull
    EBioMedicine, 2020-11-06;62(0):103077.
    Species: Human
    Sample Types: Plasma
  15. Resveratrol attenuates TLR-4 mediated inflammation and elicits therapeutic potential in models of sepsis
    Authors: B Wang, GL Bellot, K Iskandar, TW Chong, FY Goh, JJ Tai, H Schwarz, SC Wong, S Pervaiz
    Sci Rep, 2020-11-02;10(1):18837.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. An observational study of innate immune responses in patients with acute appendicitis
    Authors: T Peeters, S Martens, V D'Onofrio, MHT Stappers, JCH van der Hi, B Houben, R Achten, LAB Joosten, IC Gyssens
    Sci Rep, 2020-10-15;10(1):17352.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Stearic Acid and TNF-&alpha Co-Operatively Potentiate MIP-1&alpha Production in Monocytic Cells via MyD88 Independent TLR4/TBK/IRF3 Signaling Pathway
    Authors: S Kochumon, H Arefanian, R Azim, S Shenouda, T Jacob, N Abu Khalaf, F Al-Rashed, A Hasan, S Sindhu, F Al-Mulla, R Ahmad
    Biomedicines, 2020-10-09;8(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. MIP-1alpha Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-alpha Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress
    Authors: S Sindhu, N Akhter, A Wilson, R Thomas, H Arefanian, A Al Madhoun, F Al-Mulla, R Ahmad
    Cells, 2020-07-29;9(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case
    Authors: S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck
    Mediators Inflamm., 2020-07-06;2020(0):6087109.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnusrhamnoides (L.) A. Nelson-The Implications for Human Barrier Cells
    Authors: B Sadowska, J Rywaniak, A Cichocka, K Cichocka, J ?uchowski, U Wójcik-Boj, M Wi?ckowska, B Ró?alska
    Molecules, 2020-05-09;25(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis
    Authors: P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford
    Sci Rep, 2019-06-06;9(1):8348.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5
    Authors: G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai
    Cell Death Dis, 2019-03-19;10(4):264.
    Species: Human
    Sample Types: Serum
  23. Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine
    Authors: H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish
    Transl Oncol, 2018-12-08;12(3):397-403.
    Species: Human
    Sample Types: Serum
  24. CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
    Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
    J. Cell. Mol. Med., 2018-10-24;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads
    Authors: MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan
    PLoS ONE, 2018-01-25;13(1):e0191676.
    Species: Human
    Sample Types: Complex Sample Type
  26. Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis
    Authors: J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes
    Acta Trop., 2017-06-09;0(0):.
    Species: Human
    Sample Types: Serum
  27. Relevance of CCL3/CCR5 axis in oral carcinogenesis
    Authors: JM da Silva, TP Moreira Do, LM Sobral, CM Queiroz-Ju, MA Rachid, AEI Proudfoot, GP Garlet, AC Batista, MM Teixeira, AM Leopoldino, RC Russo, TA Silva
    Oncotarget, 2017-04-06;8(31):51024-51036.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in�vitro analysis
    Authors: Daniela Micheline Dos Santos
    J Prosthet Dent, 2016-11-09;0(0):.
  29. CD84 mediates CLL-microenvironment interactions
    Oncogene, 2016-07-25;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Neutrophil oxidative burst activates ATM to regulate cytokine production and apoptosis.
    Authors: Harbort C, Soeiro-Pereira P, von Bernuth H, Kaindl A, Costa-Carvalho B, Condino-Neto A, Reichenbach J, Roesler J, Zychlinsky A, Amulic B
    Blood, 2015-10-21;126(26):2842-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
    Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
    Reprod Biol Endocrinol, 2015-10-07;13(0):115.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014-06-09;5(0):4092.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014-05-12;9(5):e96925.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
    Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
    PLoS ONE, 2013-12-30;8(12):e82867.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.
    Authors: Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L
    J Biol Chem, 2012-11-30;288(5):3097-111.
    Species: Human
    Sample Types: CSF
  36. Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients.
    Authors: Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V
    Clinics (Sao Paulo), 2011-01-01;66(10):1699-705.
    Species: Human
    Sample Types: Plasma
  37. Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder.
    Authors: Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F
    Brain Behav. Immun., 2009-05-04;23(8):1079-82.
    Species: Human
    Sample Types: Serum
  38. Lenalidomide inhibits osteoclastogenesis, survival factors and bone-remodeling markers in multiple myeloma.
    Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Mitsiades C, Chauhan D, Okawa Y, Munshi NC, Richardson PG, Anderson KC
    Leukemia, 2008-07-03;22(10):1925-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008-07-01;8(7):1512-22.
    Species: Human
    Sample Types: BALF
  40. Clinical, radiographic, and biochemical characterization of multiple myeloma patients with osteonecrosis of the jaw.
    Authors: Raje N, Woo SB, Hande K, Yap JT, Richardson PG, Vallet S, Treister N, Hideshima T, Sheehy N, Chhetri S, Connell B, Xie W, Tai YT, Szot-Barnes A, Tian M, Schlossman RL, Weller E, Munshi NC, Van Den Abbeele AD, Anderson KC
    Clin. Cancer Res., 2008-04-15;14(8):2387-95.
    Species: Human
    Sample Types: Serum
  41. p38 mitogen-activated protein kinase inhibitor LY2228820 enhances bortezomib-induced cytotoxicity and inhibits osteoclastogenesis in multiple myeloma; therapeutic implications.
    Authors: Ishitsuka K, Hideshima T, Neri P, Vallet S, Shiraishi N, Okawa Y, Shen Z, Raje N, Kiziltepe T, Ocio EM, Chauhan D, Tassone P, Munshi N, Campbell RM, Dios AD, Shih C, Starling JJ, Tamura K, Anderson KC
    Br. J. Haematol., 2008-04-07;141(5):598-606.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Association between the macrophage inflammatory protein-l alpha gene polymorphism and Alzheimer's disease in the Chinese population.
    Authors: Li K, Dai D, Yao L, Gu X, Luan K, Tian W, Zhao Y, Wang B
    Neurosci. Lett., 2008-01-10;433(2):125-8.
    Species: Human
    Sample Types: Plasma
  43. Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages.
    Authors: Birrell MA, Wong S, Catley MC, Belvisi MG
    J. Cell. Physiol., 2008-01-01;214(1):27-37.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Increased serum levels of CCL11/eotaxin in schizophrenia.
    Authors: Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA
    Prog. Neuropsychopharmacol. Biol. Psychiatry, 2007-11-23;32(3):710-4.
    Species: Human
    Sample Types: Serum
  45. Targeting MEK1/2 blocks osteoclast differentiation, function and cytokine secretion in multiple myeloma.
    Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Chauhan D, Munshi NC, Richardson PG, Anderson KC
    Br. J. Haematol., 2007-10-01;139(1):55-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
    Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
    J. Biol. Chem., 2007-08-31;282(44):31882-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Successful HAART is associated with high B-chemokine levels in chronic HIV type 1-infected patients.
    Authors: Brito A, Almeida A, Gonsalez CR, Mendonca M, Ferreira F, Fernandes SS, Duarte AJ, Casseb J
    AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):906-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists.
    Authors: Walker J, Tough DF
    Eur. J. Immunol., 2006-07-01;36(7):1827-36.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
    Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
    J. Pharmacol. Exp. Ther., 2006-05-11;318(2):741-50.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
    Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
    Parasitology, 2005-03-01;130(0):333-42.
    Species: Human
    Sample Types: Saliva
  51. Involvement of Fc gamma RI (CD64) in the mechanism of HIV-1 inhibition by polyclonal IgG purified from infected patients in cultured monocyte-derived macrophages.
    Authors: Holl V, Hemmerter S, Burrer R, Schmidt S, Bohbot A, Aubertin AM, Moog C
    J. Immunol., 2004-11-15;173(10):6274-83.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004-02-01;55(2):221-35.
    Species: Human
    Sample Types: CSF
  53. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004-01-23;75(3):504-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Gene copy number regulates the production of the human chemokine CCL3-L1.
    Authors: Townson JR, Barcellos LF, Nibbs RJ
    Eur. J. Immunol., 2002-10-01;32(10):3016-26.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human CCL3/MIP-1 alpha DuoSet ELISA

Average Rating: 4.8 (Based on 4 Reviews)

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Human CCL3/MIP-1 alpha DuoSet ELISA
By Evan Williams on 12/12/2023
Sample Tested: Cell culture supernatant
Human CCL3/MIP-1 alpha DuoSet ELISA
By Anonymous on 02/11/2020
Sample Tested: K562 human chronic myelogenous leukemia cell line
Human CCL3/MIP-1 alpha DuoSet ELISA
By sothy yi on 12/11/2019
Sample Tested: human whole blood
Human CCL3/MIP-1 alpha DuoSet ELISA DY270
Human CCL3/MIP-1 alpha DuoSet ELISA
By Anonymous on 08/02/2016
Sample Tested: THP-1 human acute monocytic leukemia cell line
Human CCL3/MIP-1 alpha DuoSet ELISA DY270