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Human CCL27/CTACK ELISA Kit - Quantikine

Catalog #: DCC270
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DCC270
Control Products Available
QC71: Quantikine Immunoassay Control Set 1005 for Human CTACK
Human CCL27/CTACK ELISA Standard Curve
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Product Details
Procedure
Citations (10)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CCL27/CTACK Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.68 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human CTACK
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CTACK Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CTACK in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CTACK and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CTACK showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring CTACK.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 127 241 559 129 242 557
Standard Deviation 4.79 9.05 28.4 16.1 21.5 33.8
CV% 3.8 3.7 5.1 12.5 8.9 6.1

Recovery

The recovery of CTACK spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 91-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CTACK were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL27/CTACK ELISA Linearity

Scientific Data

N/A Human CCL27/CTACK ELISA Standard Curve View Larger

Human CCL27/CTACK ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL27/CTACK

CCL27, also known as Cutaneous T cell-attracting chemokine (CTACK), ALP, ILC and ESkine, is a CC chemokine discovered using expressed sequence tags (EST) in a homology search for novel chemokines. Mature human and mouse CCL27 are 84% similar. Mouse CCL27 shares 37% and 35% amino acid identity with mouse MIP-3 beta and mouse TECK, respectively. CCL27 is predominantly expressed in the skin. The expression is up-regulated by proinflammatory cytokines.

Entrez Gene IDs:
10850 (Human); 20301 (Mouse); 362505 (Rat)
Alternate Names:
ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CCL27/CTACK Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS
    Authors: T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker
    Cancer Res., 2019-02-01;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Impaired cutaneous T-cell attracting chemokine elevation and adipose-derived stromal cell migration in a high-glucose environment cause poor diabetic wound healing
    Authors: WT Wang, SS Lee, YC Wang, YW Lai, YR Kuo, YB Tang Chen, YS Liu, YC Wu
    Kaohsiung J. Med. Sci., 2018-05-30;34(10):539-546.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Phenotypic characterization of leukocytes in prenatal human dermis.
    J. Invest. Dermatol., 2012-06-21;132(11):2581-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides may be correlated with disease-free survival following treatment with interferon alfa and psoralen plus ultraviolet A therapy.
    Authors: Goteri G, Rupoli S, Campanati A, Zizzi A, Picardi P, Cardelli M, Giantomassi F, Canafoglia L, Marchegiani F, Mozzicafreddo G, Brandozzi G, Stramazzotti D, Ganzetti G, Lisa R, Simonetti O, Offidani A, Federici I, Filosa G, Leoni P
    Br. J. Dermatol., 2012-05-01;166(5):948-52.
    Species: Human
    Sample Types: Serum
  5. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009-07-22;2009(0):269541.
    Species: Human
    Sample Types: Serum
  6. Phenotype of atopic dermatitis subjects with a history of eczema herpeticum.
    Authors: Beck LA, Boguniewicz M, Hata T, Schneider LC, Hanifin J, Gallo R, Paller AS, Lieff S, Reese J, Zaccaro D, Milgrom H, Barnes KC, Leung DY
    J. Allergy Clin. Immunol., 2009-06-27;124(2):260-9, 269.e1.
    Species: Human
    Sample Types: Serum
  7. Lymphatic precollectors contain a novel, specialized subpopulation of podoplanin low, CCL27-expressing lymphatic endothelial cells.
    Authors: Wick N, Haluza D, Gurnhofer E, Raab I, Kasimir MT, Prinz M, Steiner CW, Reinisch C, Howorka A, Giovanoli P, Buchsbaum S, Krieger S, Tschachler E, Petzelbauer P, Kerjaschki D
    Am. J. Pathol., 2008-09-04;173(4):1202-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Cutting edge: rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells.
    Authors: Huang V, Lonsdorf AS, Fang L, Kakinuma T, Lee VC, Cha E, Zhang H, Nagao K, Zaleska M, Olszewski WL, Hwang ST
    J. Immunol., 2008-05-15;180(10):6462-6.
    Species: Human
    Sample Types: Serum
  9. Presence of circulating CCR10+ T cells and elevated serum CTACK/CCL27 in the early stage of mycosis fungoides.
    Authors: Sasaki M, Honda A, Furuichi M, Norisugi O
    Clin. Cancer Res., 2006-05-01;12(9):2670-5.
    Species: Human
    Sample Types: Serum
  10. Serum levels of cutaneous T-cell attracting chemokine (CTACK) as a laboratory marker of the severity of atopic dermatitis in children.
    Authors: Hon KL, Leung TF, Ma KC, Li AM, Wong Y, Fok TF
    Clin. Exp. Dermatol., 2004-05-01;29(3):293-6.
    Species: Human
    Sample Types: Serum

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