Human CCL18/PARC ELISA Kit - Quantikine

Catalog #: DCL180B
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DCL180B

Control Products Available

QC59B: Quantikine Immunoassay Control Set 868 for Human CCL18/PARC

Best Seller

Human CCL18/PARC Simple Plex Assay Linearity

3 Images

All CCL18/PARC ELISAs

Product Details

Citations (11)

Supplemental Products

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CCL18/PARC Quantikine ELISA Kit Summary

Assay Length

2.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Tissue Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)

Sensitivity

1.77 pg/mL

Assay Range

18.8 - 1,200 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human CCL18.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CCL18/PARC Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human CCL18 in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human CCL18 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CCL18 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CCL18.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	70.8	248	592	84.2	265	614
Standard Deviation	2.27	8.21	21.8	5.1	17.1	27.6
CV%	3.2	3.3	3.7	6.1	6.5	4.5

Recovery

The recovery of human CCL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	91-110
Tissue Lysis Buffer (n=1)	92	91-93
Urine (n=4)	104	92-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data

N/A

View Larger

Human CCL18/PARC ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL18/PARC

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Entrez Gene IDs:

6362 (Human)

Alternate Names:

Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CCL18/PARC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

G9a/GLP inhibition during ex vivo lymphocyte expansion increases in vivo cytotoxicity of engineered T cells against hepatocellular carcinoma
Authors: MSY Lam, JA Reales-Cal, JR Ow, JJY Aw, D Tan, R Vijayakuma, E Ceccarello, T Tabaglio, YT Lim, WL Chien, F Lai, AT Tanoto, Q Chen, RM Sobota, G Adriani, A Bertoletti, E Guccione, A Pavesi
Nature Communications, 2023-02-02;14(1):563.
Species: Human
Sample Types: Cell Culture Supernates
Exosomal lncRNA HOTAIR induce macrophages to M2 polarization via PI3K/ p-AKT /AKT pathway and promote EMT and metastasis in laryngeal squamous cell carcinoma
Authors: J Wang, N Wang, Z Zheng, Y Che, M Suzuki, S Kano, J Lu, P Wang, Y Sun, A Homma
BMC Cancer, 2022-11-24;22(1):1208.
Species: Human
Sample Types: Cell Culture Supernates
CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
Oncogene, 2022-11-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
Cell Reports, 2022-04-12;39(2):110670.
Species: Human
Sample Types: Cell Culture Supernates
The use of patient-derived breast tissue explants to study macrophage polarization and the effects of environmental chemical exposure
Authors: KJ Gregory, SM Morin, A Kubosiak, J Ser-Dolans, B Schalet, DJ Jerry, SS Schneider
Immunol. Cell Biol., 2020-09-09;0(0):.
Species: Human
Sample Types: cell culture supernatant
Repeated 5-day cycles of low dose aldesleukin in amyotrophic lateral sclerosis (IMODALS): A phase 2a randomised, double-blind, placebo-controlled trial
Authors: W Camu, M Mickunas, JL Veyrune, C Payan, C Garlanda, M Locati, R Juntas-Mor, N Pageot, A Malaspina, U Andreasson, J Kirby, C Suehs, S Saker, C Masseguin, J De Vos, H Zetterberg, PJ Shaw, A Al-Chalabi, PN Leigh, T Tree, G Bensimon
EBioMedicine, 2020-07-07;0(0):102844.
Species: Human
Sample Types: Plasma
Improving risk-stratification of rheumatoid arthritis patients for interstitial lung disease
Authors: J Avouac, A Cauvet, A Steelandt, Y Shirai, M Elhai, M Kuwana, O Distler, Y Allanore
PLoS ONE, 2020-05-08;15(5):e0232978.
Species: Human
Sample Types: Serum
M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations
Authors: A Nikonova, M Khaitov, DJ Jackson, S Traub, MB Trujillo-T, DA Kudlay, AS Dvornikov, A Del-Rosari, R Valenta, LA Stanciu, R Khaitov, SL Johnston
EBioMedicine, 2020-04-09;54(0):102734.
Species: Human
Sample Types: Cell Culture Supernates
IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages
Authors: EA Ayaub, K Tandon, M Padwal, J Imani, H Patel, A Dubey, O Mekhael, C Upagupta, A Ayoub, A Dvorkin-Gh, J Murphy, PS Kolb, S Lhotak, JG Dickhout, RC Austin, MR Kolb, CD Richards, K Ask
Immunol. Cell Biol., 2018-11-14;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Inflammation-regulating factors in ascites as predictive biomarkers of drug resistance and progression-free survival in serous epithelial ovarian cancers.
Authors: Lane D, Matte I, Garde-Granger P, Laplante C, Carignan A, Rancourt C, Piche A
BMC Cancer, 2015-07-01;15(0):492.
Species: Human
Sample Types: Peritoneal Fluid

The relationship of systemic inflammation to prior hospitalization in adult patients with cystic fibrosis.
Authors: Ngan D, Wilcox P, Aldaabil M, Li Y, Leipsic J, Sin D, Man S
BMC Pulm Med, 2012-02-14;12(0):3.
Species: Human
Sample Types: Plasma