Human Caveolin-3 Antibody Summary
Met1-Pro83
Accession # P56539
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Caveolin-3 by Western Blot. Western blot shows lysates of human heart tissue, human skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, and mouse heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-3 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Caveolin-3 in Human Skeletal Muscle. Caveolin-3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-3
Caveolin-3 is an 18 kDa member of the Caveolin family of proteins which are major protein components of plasma membrane caveolae. It is preferentially expressed in muscle cells where it regulates the localization and activity of potassium channels and the nicotinic acetylcholine receptor. Caveolin-3 mutations are associated with several skeletal muscle defects. It contains a central membrane-embedded sequence (aa 84-104) flanked by two cytoplasmic domains (aa 1-83 and 105-151). Over aa 1-83, human Caveolin-3 shares 94% aa identity with mouse and rat Caveolin-3.
Product Datasheets
Citation for Human Caveolin-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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TRPC3, but not TRPC1, as a good therapeutic target for standalone or complementary treatment of DMD
Authors: A Creisméas, C Gazaille, A Bourdon, MA Lallemand, V François, M Allais, M Ledevin, T Larcher, G Toumaniant, A Lafoux, C Huchet, I Anegon, O Adjali, C Le Guiner, B Fraysse
Journal of Translational Medicine, 2021-12-20;19(1):519.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
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