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Human Caspase-1/ICE Quantikine ELISA Kit

Catalog #: DCA100
40 Citations 3 Reviews Datasheet / COA / SDS
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DCA100
Control Products Available
QC57: Quantikine Immunoassay Control Set 775 Human Caspase-1/ICE
Human Caspase-1 ELISA Standard Curve
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Product Details
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Citations (40)
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Supplemental Products
Reviews (3)
Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Caspase-1/ICE Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL)
Sensitivity
1.24 pg/mL
Assay Range
6.3 - 400 pg/mL (Cell Culture Supernates)
Specificity
Natural and recombinant human Caspase-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Caspase-1 Immunoassay is a 3 hour solid phase ELISA designed to measure Caspase-1 in cell culture supernates. It contains recombinant human Caspase-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Caspase-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Caspase-1. These results indicate that this kit can be used to determine relative mass values for natural human Caspase-1.

Recovery

The recovery of Caspase-1 spiked to three different levels throughout the range of the assay was evaluated

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 85-106

Linearity

To assess the linearity of the assay, cell culture supernate samples containing and/or spiked with high concentrations of Caspase-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Caspase-1 ELISA Linearity

Scientific Data

N/A Human Caspase-1 ELISA Standard Curve View Larger

Human Caspase-1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Caspase-1

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

Entrez Gene IDs:
834 (Human)
Alternate Names:
CASP1 nirs variant 1; CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; Caspase-1; EC 3.4.22.36; EC:3.4.22.36; ICE; IL-1 beta-converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1.5 hours.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 100 µL Antiserum
  6.   Add 100 µL of Antiserum to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  7.   Aspirate and wash 3 times.
    8. 100 µL Conjugate
  8.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 30 minutes.
  9.   Aspirate and wash 3 times.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    11. 50 µL Stop Solution
  11. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Caspase-1/ICE Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Vaginal microbes alter epithelial transcriptomic and epigenomic modifications providing insight into the molecular mechanisms for susceptibility to adverse reproductive outcomes
    Authors: Elovitz, M;Anton, L;Cristancho, A;Ferguson, B;Joseph, A;Ravel, J;
    Research square
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Lunasin reduces the susceptibility of IL-10 deficient mice to inflammatory bowel disease and modulates the activation of the NLRP3 inflammasome
    Authors: Nieto-Veloza, A;Hong, S;Reeder, M;Sula, MJ;D'Souza, DH;Zhong, Q;Dia, VP;
    The Journal of nutritional biochemistry
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Coronary Artery Plaque Composition and Severity Relate to the Inflammasome in People With Treated Human Immunodeficiency Virus
    Authors: SR Schnittman, DW Kitch, TH Swartz, TH Burdo, KV Fitch, S McCallum, JM Flynn, ES Fulda, MR Diggs, JT Stapleton, JL Casado, J Taron, JS Currier, MV Zanni, C Malvestutt, CJ Fichtenbau, JA Aberg, HJ Ribaudo, MT Lu, PS Douglas, SK Grinspoon
    Open forum infectious diseases, 2023-03-01;10(3):ofad106.
    Species: Human
    Sample Types: Plasma
  4. Exogenous melatonin alleviates neuropathic pain-induced affective disorders by suppressing NF-kappaB/ NLRP3 pathway and apoptosis
    Authors: T Mokhtari, LP Yue, L Hu
    Scientific Reports, 2023-02-06;13(1):2111.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer's Disease Patients
    Authors: F La Rosa, CP Zoia, C Bazzini, A Bolognini, M Saresella, E Conti, C Ferrarese, F Piancone, I Marventano, D Galimberti, C Fenoglio, E Scarpini, M Clerici
    Cells, 2022-07-12;11(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. The Placental NLRP3 Inflammasome and Its Downstream Targets, Caspase-1 and Interleukin-6, Are Increased in Human Fetal Growth Restriction: Implications for Aberrant Inflammation-Induced Trophoblast Dysfunction
    Authors: I Alfian, A Chakrabort, HEJ Yong, S Saini, RWK Lau, B Kalionis, E Dimitriadi, N Alfaidy, SD Ricardo, CS Samuel, P Murthi
    Cells, 2022-04-21;11(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis
    Authors: CY Wu, WL Fan, YM Chiu, HY Yang, WI Lee, JL Huang
    Scientific Reports, 2021-06-07;11(1):11903.
    Species: Human
    Sample Types: Plasma
  8. Hydroquinone Induces NLRP3-Independent IL-18 Release from ARPE-19 Cells
    Authors: N Bhattarai, E Korhonen, Y Mysore, K Kaarnirant, A Kauppinen
    Cells, 2021-06-06;10(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes
    Authors: Y Chen, Q Fujuan, E Chen, B Yu, F Zuo, Y Yuan, X Zhao, C Xiao
    Mediators Inflamm, 2020-10-26;2020(0):1693730.
    Species: Human
    Sample Types: Serum
  10. Effects of Integrase Inhibitor-Based ART on the NLRP3 Inflammasome Among ART-Na�ve People With HIV
    Authors: M Toribio, TH Burdo, ES Fulda, M Cetlin, SM Chu, MN Feldpausch, GK Robbins, TG Neilan, K Melbourne, SK Grinspoon, MV Zanni
    Open Forum Infect Dis, 2020-09-29;7(10):ofaa459.
    Species: Human
    Sample Types: Plasma
  11. JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells
    Authors: Y Fujita, N Matsuoka, J Temmoku, M Furuya-Yas, T Asano, S Sato, H Matsumoto, H Watanabe, H Kozuru, H Yatsuhashi, A Kawakami, K Migita
    BMC Immunol., 2020-06-15;21(1):35.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. NLRP3 Inflammasome is Activated in Rat Pancreatic Islets by Transplantation and Hypoxia
    Authors: V Lavallard, D Cottet-Dum, CH Wassmer, C Rouget, G Parnaud, E Brioudes, F Lebreton, K Bellofatto, E Berishvili, T Berney, D Bosco
    Sci Rep, 2020-04-24;10(1):7011.
    Species: Rat
    Sample Types: Cell Culture Supernates
  13. Leishmania RNA virus exacerbates Leishmaniasis by subverting innate immunity via TLR3-mediated NLRP3 inflammasome inhibition
    Authors: RVH de Carvalh, DS Lima-Junio, MVG da Silva, M Dilucca, TS Rodrigues, CV Horta, ALN Silva, PF da Silva, FG Frantz, LB Lorenzon, MM Souza, F Almeida, LM Cantanhêde, RGM Ferreira, AK Cruz, DS Zamboni
    Nat Commun, 2019-11-21;10(1):5273.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. NLRP3 inflammasome is attenuated in patients with Mycobacterium avium complex lung disease and correlated with decreased interleukin-1 β response and host susceptibility
    Authors: MF Wu, CC Shu, JY Wang, BS Yan, HC Lai, BL Chiang, LS Wu, CJ Yu
    Sci Rep, 2019-08-29;9(1):12534.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Immunomodulatory effect of mancozeb, chlorothalonil, and thiophanate methyl pesticides on macrophage cells
    Authors: GCC Weis, CE Assmann, FC Cadoná, BDSR Bonadiman, AO Alves, AK Machado, MMMF Duarte, IBM da Cruz, IH Costabeber
    Ecotoxicol. Environ. Saf., 2019-07-09;182(0):109420.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Tofacitinib inhibits granulocyte-macrophage colony-stimulating factor-induced NLRP3 inflammasome activation in human neutrophils
    Authors: MY Furuya, T Asano, Y Sumichika, S Sato, H Kobayashi, H Watanabe, E Suzuki, H Kozuru, H Yatsuhashi, T Koga, H Ohira, H Sekine, A Kawakami, K Migita
    Arthritis Res. Ther., 2018-08-29;20(1):196.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Sera from Septic Patients Contain the Inhibiting Activity of the Extracellular ATP-Dependent Inflammasome Pathway
    Authors: VM Ho, N Hirohashi, WS Kong, G Yun, K Ota, J Itai, S Yamaga, K Suzuki, K Tanigawa, M Kanno, N Shime
    Tohoku J. Exp. Med., 2018-07-01;245(3):193-204.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Indoxyl Sulfate Promotes Macrophage IL-1? Production by Activating Aryl Hydrocarbon Receptor/NF-?/MAPK Cascades, but the NLRP3 inflammasome Was Not Activated
    Authors: T Wakamatsu, S Yamamoto, T Ito, Y Sato, K Matsuo, Y Takahashi, Y Kaneko, S Goto, JJ Kazama, F Gejyo, I Narita
    Toxins (Basel), 2018-03-15;10(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis
    Authors: Y Han, X Xu, C Tang, P Gao, X Chen, X Xiong, M Yang, S Yang, X Zhu, S Yuan, F Liu, L Xiao, YS Kanwar, L Sun
    Redox Biol, 2018-02-15;16(0):32-46.
    Species: Human
    Sample Types: Cell Lysates
  20. The NLRP3 Inflammasome Is Upregulated in HIV-Infected Antiretroviral Therapy-Treated Individuals with Defective Immune Recovery
    Authors: A Bandera, M Masetti, M Fabbiani, M Biasin, A Muscatello, N Squillace, M Clerici, A Gori, D Trabattoni
    Front Immunol, 2018-02-12;9(0):214.
    Species: Human
    Sample Types: Plasma
  21. Withaferin A Associated Differential Regulation of Inflammatory Cytokines
    Authors: S Dubey, H Yoon, MS Cohen, P Nagarkatti, M Nagarkatti, D Karan
    Front Immunol, 2018-02-09;9(0):195.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Inhibition of the inflammatory response to stress by targeting interaction between PKR and its cellular activator PACT
    Authors: S Dabo, P Maillard, M Collados R, MD Hansen, S Mazouz, DJ Bigot, M Tible, G Janvier, O Helynck, P Cassonnet, Y Jacob, J Bellalou, A Gatignol, RC Patel, J Hugon, H Munier-Leh, EF Meurs
    Sci Rep, 2017-11-23;7(1):16129.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. NLRP3 Inflammasome Activation in THP-1 Target Cells Triggered by Pathogenic Naegleria fowleri
    Authors: JH Kim, HJ Sohn, JK Yoo, H Kang, GS Seong, YJ Chwae, K Kim, S Park, HJ Shin
    Infect. Immun, 2016-08-19;84(9):2422-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. F-actin dampens NLRP3 inflammasome activity via Flightless-I and LRRFIP2
    Sci Rep, 2016-07-19;6(0):29834.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1.
    Authors: Shamaa O, Mitra S, Gavrilin M, Wewers M
    PLoS ONE, 2015-11-24;10(11):e0142203.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Pseudomonas aeruginosa Triggers Macrophage Autophagy To Escape Intracellular Killing by Activation of the NLRP3 Inflammasome.
    Authors: Deng Q, Wang Y, Zhang Y, Li M, Li D, Huang X, Wu Y, Pu J, Wu M
    Infect Immun, 2015-10-14;84(1):56-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Resolvins Decrease Oxidative Stress Mediated Macrophage and Epithelial Cell Interaction through Decreased Cytokine Secretion.
    Authors: Cox R, Phillips O, Fukumoto J, Fukumoto I, Tamarapu Parthasarathy P, Mandry M, Cho Y, Lockey R, Kolliputi N
    PLoS ONE, 2015-08-28;10(8):e0136755.
    Species: Human
    Sample Types: Cell Lysates
  28. Cross talk between neuroregulatory molecule and monocyte: nerve growth factor activates the inflammasome.
    Authors: Datta-Mitra A, Kundu-Raychaudhuri S, Mitra A, Raychaudhuri S
    PLoS ONE, 2015-04-15;10(4):e0121626.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Aggregatibacter actinomycetemcomitans cytolethal distending toxin activates the NLRP3 inflammasome in human macrophages, leading to the release of proinflammatory cytokines.
    Authors: Shenker B, Ojcius D, Walker L, Zekavat A, Scuron M, Boesze-Battaglia K
    Infect Immun, 2015-02-02;83(4):1487-96.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. CREB-induced inflammation is important for malignant mesothelioma growth.
    Authors: Westbom C, Shukla A, MacPherson M, Yasewicz E, Miller J, Beuschel S, Steele C, Pass H, Vacek P, Shukla A
    Am J Pathol, 2014-08-08;184(10):2816-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Microvesicular caspase-1 mediates lymphocyte apoptosis in sepsis.
    Authors: Exline M, Justiniano S, Hollyfield J, Berhe F, Besecker B, Das S, Wewers M, Sarkar A
    PLoS ONE, 2014-03-18;9(3):e90968.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
    Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
    Blood, 2012-11-14;121(1):95-106.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. The anti-tumorigenic mushroom Agaricus blazei Murill enhances IL-1beta production and activates the NLRP3 inflammasome in human macrophages.
    Authors: Huang T, Ojcius D, Young J, Wu Y, Ko Y, Wong T, Wu C, Lu C, Lai H
    PLoS ONE, 2012-07-23;7(7):e41383.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
    Authors: Beynon V, Quintana FJ, Weiner HL
    PLoS ONE, 2012-06-29;7(6):e39576.
    Species: Human
    Sample Types: Serum
  35. HMG-CoA reductase inhibition induces IL-1beta release through Rac1/PI3K/PKB-dependent caspase-1 activation.
    Authors: Kuijk LM, Beekman JM, Koster J, Waterham HR, Frenkel J, Coffer PJ
    Blood, 2008-08-06;112(9):3563-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Haplotypes of the caspase-1 gene, plasma caspase-1 levels, and cardiovascular risk.
    Authors: Blankenberg S, Godefroy T, Poirier O, Rupprecht HJ, Barbaux S, Bickel C, Nicaud V, Schnabel R, Kee F, Morrison C, Evans A, Lackner KJ, Cambien F, Munzel T, Tiret L
    Circ. Res., 2006-06-15;99(1):102-8.
    Species: Human
    Sample Types: Plasma
  37. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006-01-15;12(2):376-82.
    Species: Human
    Sample Types: Plasma
  38. The effect of the physical characteristics of hydroxyapatite particles on human monocytes IL-18 production in vitro.
    Authors: Grandjean-Laquerriere A, Laquerriere P, Laurent-Maquin D, Guenounou M, Phillips TM
    Biomaterials, 2004-12-01;25(28):5921-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Evaluation of caspase 1 and sFas serum levels in patients with systemic sclerosis: correlation with lung dysfunction, joint and bone involvement.
    Authors: Dziankowska-Bartkowiak B, Waszczykowska E, Zalewska A, Sysa-Jedrzejowska A
    Mediators Inflamm., 2003-12-01;12(6):339-43.
    Species: Human
    Sample Types: Serum
  40. Differential effect of serotonin on cytokine production in lipopolysaccharide-stimulated human peripheral blood mononuclear cells: involvement of 5-hydroxytryptamine2A receptors.
    Authors: Cloez-Tayarani I, Petit-Bertron AF, Venters HD, Cavaillon JM
    Int. Immunol., 2003-02-01;15(2):233-40.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human Caspase-1/ICE Quantikine ELISA Kit

Average Rating: 4.7 (Based on 3 Reviews)

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Human Caspase-1/ICE Quantikine ELISA Kit
By Phoibe Renema on 12/02/2017
Sample Tested: Human Plasma
Human Caspase-1/ICE Quantikine ELISA Kit
By Anonymous on 06/01/2017
Sample Tested: Cell Culture Supernates
Human Caspase-1/ICE Quantikine ELISA Kit DCA100
Human Caspase-1/ICE Quantikine ELISA Kit
By Anonymous on 05/10/2016
Sample Tested: Human plasma EDTA
Human Caspase-1/ICE Quantikine ELISA Kit DCA100