Human Carbonic Anhydrase VI Antibody

Catalog #: MAB2939 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2939
MAB2939-SP

Detection of Human Carbonic Anhydrase VI by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase VI Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Carbonic Anhydrase 2, 3, 4, 5A, 5B, 7, 8, 9, recombinant mouse Carbonic Anhydrase 12, and 14 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 401819

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carbonic Anhydrase VI by Western Blot. Western blot shows human saliva. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Carbonic Anhydrase VI by Simple Western^TM. Simple Western lane view shows human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carbonic Anhydrase VI Monoclonal Antibody (Catalog # MAB2939). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5). Mature human CA6 shares 66% and 50% amino acid sequence identity with bovine and mouse CA6, respectively.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.

Entrez Gene IDs

765 (Human); 298657 (Rat)

Alternate Names

CA6; Carbonate dehydratase VI; carbonic anhydrase 6; Carbonic Anhydrase VI; carbonic anhydrase VIGUSTIN; CA-VI; EC 4.2.1.1; Gustin; MGC21256; Salivary carbonic anhydrase; Secreted carbonic anhydrase