Human CaM Kinase II alpha Antibody

Catalog #: MAB5584 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5584
MAB5584-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CaM Kinase II alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CaM Kinase II alpha in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 525501

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CaM Kinase II alpha
Ala144-Ala262
Accession # Q9UQM7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase type II alpha (CaMKII alpha ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKII alpha is predominantly expressed in the brain. It shares 100% aa sequence identity between human, mouse, and rat within aa 144‑262 of isoform 1.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Entrez Gene IDs

815 (Human); 12322 (Mouse); 25400 (Rat)

Alternate Names

calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; CaM kinase II alpha subunit; CaM Kinase II alpha; CaM kinase II subunit alpha; CAMK2A; CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha; CaMK-II alpha subunit; CaMK-II subunit alpha; CaMKIINalpha; CaM-kinase II alpha chain; EC 2.7.11; EC 2.7.11.17; KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain