Home / BAI3 / Human BAI3 Antibody

Human BAI3 Antibody

Catalog #: MAB3965 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3965
MAB3965-SP
Detection of BAI3 in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BAI3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BAI3 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 409611
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BAI3
Ala25-Thr880
Accession # O60242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BAI3 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of BAI3 antibody in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP antibody by Flow Cytometry. View Larger

Detection of BAI3 in HEK293 Human Cell Line Transfected with Human BAI3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human BAI3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human BAI3 Monoclonal Antibody (Catalog # MAB3965) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAI3

Human BAI3 (brain-specific angiogenesis inhibitor 3) is a 177 kDa, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25‑883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296‑508), and one GPS domain (aa 816‑867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643‑665. Over aa 25‑880, human BAI3 shares 98% aa identity with mouse BAI3.

Long Name
Brain-specific Angiogenesis Inhibitor 3
Entrez Gene IDs
577 (Human); 210933 (Mouse); 301309 (Rat)
Alternate Names
BAI3; brain-specific angiogenesis inhibitor 3; dJ91B17.1 (brain-specific angiogenesis inhibitor 3); KIAA0550MGC133100

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human BAI3 Antibody

There are currently no reviews for this product. Be the first to review Human BAI3 Antibody and earn rewards!

Have you used Human BAI3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review