Human B7-H3 MAb (Clone 185504) Summary
Leu29-Pro245
Accession # NP_079516
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human B7‑H3 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human B7-H3 Monoclonal Antibody (Catalog # MAB1027) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7-H3 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of B7-H3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 Monoclonal Antibody (Catalog # MAB1027, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
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Detection of B7‑H3 in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human B7-H3 Monoclonal Antibody (Catalog # MAB1027, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
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Western Blot Shows Human B7‑H3 Specificity by Using Knockout Cell Line. Western blot shows lysates of U2OS human osteosarcoma parental cell line and B7-H3 knockout U2OS cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human B7-H3 Monoclonal Antibody (Catalog # MAB1027) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H3
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses (1‑3). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20‑40% amino acid (aa) sequence identity. The cloned human B7-H3 cDNA encodes a 316 aa type I membrane precursor protein with a putative 28 aa signal peptide, a 217 aa extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a 45 aa cytoplasmic domain. An isoform of human B7-H3 containing a four-Ig-like domain extracellular region has also been identified. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines. B7-H3 expression is also detected on various normal tissues and in some tumor cell lines. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptors. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1‑3).
- Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
- Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
- Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Product Datasheets
Citations for Human B7-H3 MAb (Clone 185504)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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CAR T Cells Targeting B7-H3, a Pan-Cancer Antigen, Demonstrate Potent Preclinical Activity Against Pediatric Solid Tumors and Brain Tumors
Authors: Robbie G. Majzner, Johanna L. Theruvath, Anandani Nellan, Sabine Heitzeneder, Yongzhi Cui, Christopher W. Mount et al.
Clinical Cancer Research
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Prognostic Role of Soluble and Extracellular Vesicle-Associated PD-L1, B7-H3 and B7-H4 in Non-Small Cell Lung Cancer Patients Treated with Immune Checkpoint Inhibitors
Authors: C Genova, R Tasso, A Rosa, G Rossi, D Reverberi, V Fontana, S Marconi, M Croce, MG Dal Bello, C Dellepiane, M Tagliament, MC Ciferri, L Zullo, A Fedeli, A Alama, K Cortese, C Gentili, E Cella, G Anselmi, M Mora, G Barletta, E Rijavec, F Grossi, P Pronzato, S Coco
Cells, 2023-03-08;12(6):.
Species: Human
Sample Types: Extracellular Vesicle Lysate
Applications: Western Blot -
Evaluation of an Affibody-Based Binder for Imaging of Immune Check-Point Molecule B7-H3
Authors: M Oroujeni, EA Bezverkhni, T Xu, Y Liu, EV Plotnikov, I Karlberg, E Ryer, A Orlova, V Tolmachev, FY Frejd
Pharmaceutics, 2022-08-25;14(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting B7-H3 via chimeric antigen receptor T cells and bispecific killer cell engagers augments antitumor response of cytotoxic lymphocytes
Authors: J Liu, S Yang, B Cao, G Zhou, F Zhang, Y Wang, R Wang, L Zhu, Y Meng, C Hu, H Liang, X Lin, K Zhu, G Chen, KQ Luo, L Di, Q Zhao
Journal of hematology & oncology, 2021-01-29;14(1):21.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Targeting B7-H3 Immune Checkpoint With Chimeric Antigen Receptor-Engineered Natural Killer Cells Exhibits Potent Cytotoxicity Against Non-Small Cell Lung Cancer
Authors: S Yang, B Cao, G Zhou, L Zhu, L Wang, L Zhang, HF Kwok, Z Zhang, Q Zhao
Front Pharmacol, 2020-07-30;11(0):1089.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Genetic evolution of T-cell resistance in the course of melanoma progression.
Authors: Sucker A, Zhao F, Real B, Heeke C, Bielefeld N, Mabetaen S, Horn S, Moll I, Maltaner R, Horn P, Schilling B, Sabbatino F, Lennerz V, Kloor M, Ferrone S, Schadendorf D, Falk C, Griewank K, Paschen A
Clin Cancer Res, 2014-10-07;20(24):6593-604.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer.
Authors: Ramsay, Alan G, Clear, Andrew J, Fatah, Rewas, Gribben, John G
Blood, 2012-04-30;120(7):1412-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Authors: Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
J. Immunol., 2008-03-01;180(5):2989-98.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumor cell and tumor vasculature expression of B7-H3 predict survival in clear cell renal cell carcinoma.
Authors: Crispen PL, Sheinin Y, Roth TJ, Lohse CM, Kuntz SM, Frigola X, Thompson RH, Boorjian SA, Dong H, Leibovich BC, Blute ML, Kwon ED
Clin. Cancer Res., 2008;14(16):5150-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection.
Authors: Das S, Suarez G, Beswick EJ, Sierra JC, Graham DY, Reyes VE
J. Immunol., 2006-03-01;176(5):3000-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Expression of B7-H3, a Potential Factor of Tumor Immune Evasion in Combination with the Number of Regulatory T Cells, Affects Against Recurrence-Free Survival in Breast Cancer Patients
Authors: N. Maeda, K. Yoshimura, S. Yamamoto, A. Kuramasu, M. Inoue, N. Suzuki et al.
Annals of Surgical Oncology
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