Home / B7-H3 / Human B7-H3 Fluorescein-conjugated Antibody

Human B7-H3 Fluorescein-conjugated Antibody

Catalog #: FAB1027F
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1027F
Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-H3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.
Source
Monoclonal Mouse IgG1 Clone # 185504
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7‑H3
Leu29-Pro245
Accession # NP_079516
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B7-H3 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of B7‑H3 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human B7-H3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1027F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H3

B7-H3 is a member of the B7 family of immune costimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

Long Name
B7 Homolog 3
Entrez Gene IDs
80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule

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Citations for Human B7-H3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma
    Authors: J Xie, M Sun, D Zhang, C Chen, S Lin, G Zhang
    FEBS Open Bio, 2021-10-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation, Western Blot
  2. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
    Authors: BD Hock, SA MacPherson, JL McKenzie
    PLoS ONE, 2017-03-03;12(3):e0172858.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The cell surface proteome of human mesenchymal stromal cells.
    Authors: Niehage C, Steenblock C, Pursche T, Bornhauser M, Corbeil D, Hoflack B
    PLoS ONE, 2011-05-26;6(5):e20399.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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