Human Apolipoprotein C-II/ApoC2 Antibody

Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot. Western blot shows lysates of human liver tissue and human plasma. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Apolipoprotein C‑II/ApoC2 in THP‑1 Human Cell Line. Apolipoprotein C-II/ApoC2 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (positive stain) and MCF-7 human breast cancer cell line (negative stain) using Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Apolipoprotein C‑II/ApoC2 in Human Liver. Apolipoprotein C-II/ApoC2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Apolipoprotein C-II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human Apolipoprotein C‑II/ApoC2 ELISA Standard Curve. Recombinant Human Apolipoprotein C‑II/ApoC2 protein was serially diluted 2-fold and captured by Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (Catalog # MAB4497) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Human Apolipoprotein C‑II/ApoC2 Monoclonal Antibody (MAB44971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).